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EMAGE:20723

Mfhas1 malignant fibrous histiocytoma amplified sequence 1 ( MGI:1098644)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20723 EMAGE:20723 EMAGE:20723 EMAGE:20723 EMAGE:20723
"Pseudo-wholemount" of euxassay_014051. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014051_01 euxassay_014051_02 euxassay_014051_03 euxassay_014051_04
EMAGE:20723 EMAGE:20723 EMAGE:20723 EMAGE:20723 EMAGE:20723
euxassay_014051_05 euxassay_014051_06 euxassay_014051_07 euxassay_014051_08 euxassay_014051_09
EMAGE:20723 EMAGE:20723 EMAGE:20723 EMAGE:20723 EMAGE:20723
euxassay_014051_10 euxassay_014051_11 euxassay_014051_12 euxassay_014051_13 euxassay_014051_14
EMAGE:20723 EMAGE:20723 EMAGE:20723 EMAGE:20723 EMAGE:20723
euxassay_014051_15 euxassay_014051_16 euxassay_014051_17 euxassay_014051_18 euxassay_014051_19
EMAGE:20723 EMAGE:20723 EMAGE:20723
euxassay_014051_20 euxassay_014051_21 euxassay_014051_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20723Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20723_wholemount_strong.wlz
20723_wholemount_moderate.wlz
20723_wholemount_weak.wlz
20723_wholemount_possible.wlz
20723_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20723_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forebrain
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
vibrissa
weak weak
regionalweak expression: see section 04 05 20 21 22
hindbrain
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
midbrain
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 18 19
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 07 16 17
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21
spinal cord
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12 13 14
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 10 11 12 13 14 15 16
neural retina
weak weak
regionalweak expression: see section 01 02 03 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40117
Entity Detected:Mfhas1, malignant fibrous histiocytoma amplified sequence 1 ( MGI:1098644)
Sequence:sense strand is shown

>T40117
ACGCTGTGGTGTGTATTGTAGGCACGCATGCGGACCTGTGTGGGGAGCGGGAGCTTGAGGAGAAATGTCT
GGACATTCACCGACAGATTGCCCTGCAGGAGAAGAACGATGCAGAGGGCCTTAGCCATCTAGCCAAGGTG
GTGGATGAGGCCCTGGCTCGGGACTTCGAGCTGCGCTCTGCCAGCCCTCACGCAGCCTACTACGGTGTTT
CAGACAAGAACCTTCGGAGGCGCAAGGCCCATTTTCAATACTTGCTTAACCACCGGTTGCAGATCCTCTC
TCCCGTGTTGCCCGTAAGCTGCCGGGACCCCCTCCAATTGCAACGCCTTCGAGACAAGCTGCTCTCTGTA
GCTGAGCACCGAGAAATCTTTCCCAACTTACACCGAGTCCTGCCTCGATCCTGGCAGGTACTGGAGGAAC
TGCACTTCCAGCCACCTCAGGCACAACGACTGTGGCTGAGCTGGTGGGACTCTGCCCGCCTGGGCCTGCA
GGCAGGTCTGACTGAGGACCGGCTCCAGAGTGCTCTTTCCTACCTGCACGAGAGCGGCAAACTGCTCTAT
TTTGAGGATAGCCCAGCCCTCAAGGAGCATGTCTTCCACAACCTCACCCGCCTCATTGACATCCTCAATG
TCTTTTTCCAGAGAGATGCTTCATTGCTGCTGCATAAACTGCTCTTAGGGACCAATGGAGAGGGGGAGGG
GGAGGGGGAAAGTTTCCCCACTATAGCAGTGCCCAGCCCGGGTCAGGACCCACTCCGGGCCACCCAGCTT
CATCATTATGTGGAAGGCTTTTTGCTGCATGGGCTTCTGCCAGCCCACATCATTCGGTTGCTGCTTAAGC
CTCATGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81258. Forward Primer - name:081258_F_cDNA_Mfhas1, sequence:ACGCTGTGGTGTGTATTGTAGG; Reverse Primer - name:081258_N_SP6_cDNA_Mfhas1, sequence:ACATGAGGCTTAAGCAGCAACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20721 same embryo
 EMAGE:20720 same embryo
 EMAGE:20725 same embryo
 EMAGE:20724 same embryo
 EMAGE:20722 same embryo
 EurExpress:euxassay_014051 same experiment
 MGI:4826178 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS