EMAGE mouse gene expression spatial database

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EMAGE:21516

Exosc1 exosome component 1 ( MGI:1913833)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003018_01	euxassay_003018_02	euxassay_003018_03	euxassay_003018_04	euxassay_003018_05

euxassay_003018_06	euxassay_003018_07	euxassay_003018_08	euxassay_003018_09	euxassay_003018_10

euxassay_003018_11	euxassay_003018_12	euxassay_003018_13	euxassay_003018_14	euxassay_003018_15

euxassay_003018_16	euxassay_003018_17	euxassay_003018_18	euxassay_003018_19	euxassay_003018_20

euxassay_003018_21	euxassay_003018_22	euxassay_003018_23	euxassay_003018_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21516_wholemount_strong.wlz

21516_wholemount_moderate.wlz

21516_wholemount_weak.wlz

21516_wholemount_possible.wlz

21516_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21516_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 6 7 8 9 0 6 8 weak expression: see section 7 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4654
Entity Detected:	Exosc1, exosome component 1 ( MGI:1913833)
Sequence:	sense strand is shown >T4654 ATGGTGGCAAGCATGGCACCGCCGGTGAGGTACTGCATCCCCGGCGAACGTCTGTGTAACTTGGAGGAAG GCAGCCCCGGCAGCGGCACCTACACCCGGCATGGCTACATCTTTTCGTCGCTGGCTGGCTGCCTGATGAA GACCAGCGAGAATGGCGCGGTTCCCGTGGTGTCAGTGATGAGAGAAACAGAGTCCCAGTTGCTTCCAGAT GTGGGAGCTGTCGTCACCTGTAAGGTCTCTAGCATCAACTCACGGTTTGCCAAAGTACACATCCTGTATG TGGGATCCACACCACTCAAAAATGCTTTTCGAGGAACTATCCGCAAAGAAGATATCCGAGCAACTGAAAA AGACAAGGTAGAAATTTACAAGAGTTTTCGGCCAGGTGACATAGTTTTGGCCAAAGTTATCTCCCTAGGC GATGCACAGTCCAATTACCTGCTGACTACTGCTGAAAACGAGCTGGGCGTTGTGGTGGCCCACAGTGAGT CAGGTGTTCAGATGGTTCCCATCAGCTGGTGTGAGATGCAGTGCCCCAAGACCCACACTAAAGAATTCCG AAAAGTGGCCCGAGTACAGCCCGAGTTCCTACAGACCTAAGCCCACCTACCCCAAAGAGGACTTCAGCAC TTCAGCTACTTCTAAAAGTACACATATAAAAAAACCATCAAGATGCAATGGTCTTTATTAAGGTACCTGC GGTCAGCTGGCAACCATCTCTTGAAACAATGTGCCGGAAATGTATTCATGCTGTAATGACACCCAAGGCC CCTGCCGCTGCTGAGACAGGGAGATTGTTAGTGCCCAAGTTTGAGGCCAGCCTGGGCTACATATTAAGAC TCTTTCAACCAAAAGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5010850 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010850 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003018 same experiment
	EMAGE:21515 same embryo
	EMAGE:21517 same embryo
	EMAGE:21518 same embryo
	EMAGE:21513 same embryo
	EMAGE:21514 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source