EMAGE mouse gene expression spatial database

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EMAGE:21518

Letmd1 LETM1 domain containing 1 ( MGI:1915864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001108. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001108_01	euxassay_001108_02	euxassay_001108_03	euxassay_001108_04

euxassay_001108_05	euxassay_001108_06	euxassay_001108_07	euxassay_001108_08	euxassay_001108_09

euxassay_001108_10	euxassay_001108_11	euxassay_001108_12	euxassay_001108_13	euxassay_001108_14

euxassay_001108_15	euxassay_001108_16	euxassay_001108_17	euxassay_001108_18	euxassay_001108_19

euxassay_001108_20	euxassay_001108_21	euxassay_001108_22	euxassay_001108_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21518_wholemount_strong.wlz

21518_wholemount_moderate.wlz

21518_wholemount_weak.wlz

21518_wholemount_possible.wlz

21518_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21518_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

homogeneous

moderate expression: see section 4 5 6 9 0 1

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 7 8

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 3 4 5 6 7 8 9 7 8 9 0 1 2

vagus x ganglion

moderate

homogeneous

moderate expression: see section 8 9 7

vestibulocochlear viii ganglion

weak

homogeneous

weak expression: see section 6 7 8

dorsal root ganglion

moderate

regional

moderate expression: see section 8 9 0 1 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4652
Entity Detected:	Letmd1, LETM1 domain containing 1 ( MGI:1915864)
Sequence:	sense strand is shown >T4652 CCACGCGTCCGGCGTGCGCCACCACCGCCCCGCAGCATGGCCCTTTTCGTAGGCTGCAGTATAAGCTCAT TTTAAGTGAGTGGGATGGAGGGATTGGCCACATTATCAGGAACTCACCCCAGTTAAAATGGGATCTGTTT ATTTCTGTTTAGTTCCGACGAGATATCACCAAGTGTCTCTTCGTTGGCCTCATCTCCATTCCACCCTTTG CCAACTACCTGGTCTTTTTGCTAATGTACCTGTTTCCTAGGCAACTGCTAGTCAAGCATTTCTGGACCCC CAAACAACAAATTGATTTCTTGGACGTTTATCATGGTCTCCGGAGGCGGTCCCACTCGGAAGTCATCACA CATCTAAGAAGGGCCAGCACTTTTGTTTCTCATGAGAAACTCCGGAGGCAGCTGACAGACCTGTGCACCA AGGTGCAGAGTGGCACCCACCCAGCAGCACAGGACGTCCTGGCTCTCAGAGATTGCTTCTCTACCTACCC CTTGGGCTTCAGCCAGCTCCAAGCCTCGCAGATGAGAGCCTTGAGCCAGGCCATGCTCCTCACACCTTAC CTGCCCCCGCCCTTGCTGAGGCAGCGGCTAAAGAGTCACACCACCGTGATCCACCAGCTGGACAGGGCTC TGGCAAAGCTGGGGATTGGCCAGCTGACTGCACAGGAAGTGAAATCGGCTTGCTATCTTCGTGGCTTGAA CTCTACCCACATTGCTGACGACAGGTGTCGAGCCTGGCTGGGGGAATGGTTGCACATCTCCTGTAGCCTG AAAGAACCCGAGCTGTCCCTCTTGCTGCACAACGTGGTCCTGCTCTCCACCAACTACCTTGAGACAAGGC GCTGAGTGAACCCCACGGAAAGGACGGCATTGTCCTGCAGTCACCTAGTGTAGTGGTGTGGGACCAAGAA GCACCTGTCAGCTGTCTGTGCGCACCACTAGTGTGGGAGGCAGCGGAGTGGGTTCCACGGGCTTCAGAGC ACGCTACCCCCCTGGGAGCTATAGACATTCCTTCACAGCTTGAGCCTAACAAACCCATGTGTGCTAACCT GGAACTGGCAAGCATCTCTGGTACTGAACTTTTATTGTGATGTAATACTTAGGTCCTATAAAGTCAGCCT TTGCCCAGGTGGGAATCATGTGGCCTAAAATTGAGCACTGCCATGGTTTTGTTTTTAAGGTGTTTTTTGT GTGTTCCTTTTTGAGATGAGTCTCTGTTGCCCAGGCTGGCCTCCAATCCCTGTGCTCAAGGGATCCTCTC TCTCCAGACTCGGGTGCTGCGGCTGCACTGGCCTCACTGCCACGTTCTCTACAGCCGAGCCTTGTTTTCC TGAGACCCCGTGTCTCATGTAGCCGAAGAGGACCTTGAGATCTGGTCTTCCTACCCCCAGCTCCCAGGCG CTGGAACACCGGCAAGGAGCCCACGAAGCTCTGGGGTGTTGTCTTGGCTGAGTGCTTTTTACCGAGTCTT TGTGCACTAGCGGATCTCTGGTACCAAGAACAGTGCAGCTTACTCTGGGGTGAGGGCACCCATGCCTGGC CCTGCGCCAACACCCCGGCAGTGGTTTTTCTTATGCCCCCTCCCTTTTCCTTTTATGGCCTTTTTGAGAG TCTTACTGTATAACCTGGCAATCCTGACAGACAATTCTGCCACAGCCTCCTGGAGCGAGAATATAAGCAT GAGCCACACCCATCCTTTCTGGTGAGACCAAAAGTAGGGCTGTTTCTCCTCGTCACTATCATGGGCTAAC TTCCACCCCTCCTCTCAGTATTCTATGGACACTTTTTTTTTTTTAATAGTAATTTATGGGAACATTCTAA AGGAGGCGGTGAGAAAGAATGGCTGCCTGGCCCCTCCCTGCGTTTGTCCCCAGTGTCTTACTTTCCAGGC TGTCACACAGCCCCTAGGGACCTTACATGTTTGCTACCTCCACTGTGTTGCACTAAGCCATCTGTAACCA GCAGTGGCTGCCTTCTCTAGGCTGTGGGTCAAAAACGCACAAGGTACTAATTACTGCCCAGCCCTGGGAG ACCAGGAGATTTGCAATAGATAGTGAGTTGGGCTTAACTGTCGTGTGTACATCTATGCCCTGTCGTTTTG TAATAACTTACTGTAAATGCATGAAGCACTGGTTTAAGCCTAAGTAAAGACTGCCTGAAACCAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5010892 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010892 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001108 same experiment
	EMAGE:21515 same embryo
	EMAGE:21517 same embryo
	EMAGE:21513 same embryo
	EMAGE:21514 same embryo
	EMAGE:21516 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source