EMAGE mouse gene expression spatial database

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EMAGE:21517

Acp1 acid phosphatase 1, soluble ( MGI:87881)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001099. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001099_01	euxassay_001099_02	euxassay_001099_03	euxassay_001099_04

euxassay_001099_05	euxassay_001099_06	euxassay_001099_07	euxassay_001099_08	euxassay_001099_09

euxassay_001099_10	euxassay_001099_11	euxassay_001099_12	euxassay_001099_13	euxassay_001099_14

euxassay_001099_15	euxassay_001099_16	euxassay_001099_17	euxassay_001099_18	euxassay_001099_19

euxassay_001099_20	euxassay_001099_21	euxassay_001099_22	euxassay_001099_23	euxassay_001099_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21517_wholemount_strong.wlz

21517_wholemount_moderate.wlz

21517_wholemount_weak.wlz

21517_wholemount_possible.wlz

21517_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21517_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6

submandibular gland primordium

moderate

regional

moderate expression: see section 7 8 9 0 9 0

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

liver lobe

weak

homogeneous

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

kidney calyx

weak

homogeneous

weak expression: see section 7 8 9 0 1 7 8 9 0 1

chondrocranium

weak

regional

weak expression: see section 1 2 3 4 5 6 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4650
Entity Detected:	Acp1, acid phosphatase 1, soluble ( MGI:87881)
Sequence:	sense strand is shown >T4650 GGGCGCCAGGGTCTGCACCGAAACATGGCAGAGGTTGGGTCCAAGTCAGTGCTGTTCGTGTGTCTCGGTA ACATTTGCCGGTCACCCATTGCAGAAGCAGTATTCAGGAAACTGGTAACTGATGAAAAGGTTTCAGATAA TTGGAGGATAGACAGTGCGGCTACATCCACCTATGAAGTGGGGAACCCTCCTGACTATCGAGGGCAGAAC TGCATGAGAAAACATGGCATCCACATGCAGCACATTGCACGGCAGATTACAAAAGAAGACTTTGCCACAT TCGATTATATACTATGTATGGATGAAAGCAATCTGAGAGATCTCAATAGAAAAAGTAATCAAGTTAAAAA CTGCAAAGCTAAAATTGAGCTACTTGGGAGCTATGATCCACAGAAACAGCTCATCATTGAAGATCCCTAT TATGGCAATGACTCTGACTTCGAGGTGGTGTACCAGCAATGCCTTAGGTGCTGCAAGGCCTTCCTGGAGA AGACTTACTAGCTGGTCCTAAGCCCCACCATTGAGCAGCTCACTCATCAGTGCTGTGCCCAAGGGTGGTG GCAGTCCTTAGCCCCATACCCCATCTCTCTTTTCAGCTAACTTACTGTGTATCTTTAAAATAATTGTAGG TGGGAATTAGGCATATGTTCAGAAGGATAAAAGCATTTGAGTCAGACAGTTTGAGGTGTGGCTAAGCATT CTTAGACTAACTAAACCTCTGACCTTGCGGTGATTACAAAACAGTGGAACAAGCAAATATGGAACAAAAA AACAAAAACAAAAACAAAAACCCAGTAAAGTAAGAGTGACCTAGAAGGTCCAGATCAGCCTCTGAGCTCG GCAAGCCTGGGTCGTCTGGTCTAAGTGGAGTGTGTGCATGACCCACACCCAGTGTGCTGTTTGCTTGCCG TGTACTCTCTACTCTAGGCTCTCGTTGTGACAATAGCTCCATTCACGGCAGCCTTCCAGTTAACGCTGGC AGTTTAAGCTCAGACACACTGAGGGTGTTGAGGGATTTGAGAGAGGACACGCTGGATGTGCTGATGGGCC TGGGACTCCAGCAGGCCGTCCTGGGCTGGACAGTGACACCCTGTCTAAAAGGGGAACAAAAATAACAACG GATGGGAGCAGCAGATGGATAAAGTTACCATCTTCAGTCTTACTTGGTTTTTGTCTACTTTCCTGAGCTT TGTTCTTGTTTGAGCCACTTTGCTTTAAAAAAAAAGTAAGTGTGCTTAAATACTGGTATGTGTTTGTAAG TGTTCTCTCATCGGCAATTTACAAAGTTATAGGCAAGCATGAGTAATTTTTGTGTATTTTCAGAAAAGAG ACCTCAAATTTATATGAATGTATGTCAGAAAGGAACATGAATTCAACTGGGAAGTTATCAAATACAACTG AGATACAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5010346 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5010346 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001099 same experiment
	EMAGE:21515 same embryo
	EMAGE:21518 same embryo
	EMAGE:21513 same embryo
	EMAGE:21514 same embryo
	EMAGE:21516 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source