EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29400

Wfdc5 ( MGI:2384800)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003779_01	euxassay_003779_02	euxassay_003779_03	euxassay_003779_04	euxassay_003779_05

euxassay_003779_06	euxassay_003779_07	euxassay_003779_08	euxassay_003779_09	euxassay_003779_10

euxassay_003779_11	euxassay_003779_12	euxassay_003779_13	euxassay_003779_14	euxassay_003779_15

euxassay_003779_16	euxassay_003779_17	euxassay_003779_18	euxassay_003779_19	euxassay_003779_20

euxassay_003779_21	euxassay_003779_22	euxassay_003779_23	euxassay_003779_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6970
Entity Detected:	Wfdc5, ( MGI:2384800)
Sequence:	sense strand is shown >T6970 CTGACTCAACTGCTCTGGCATCTCTGAGACTGTAAATGTTAATCTGGGGGCCTCTGAGCTCTGAGCTTAT TGGGTGCCACGAAAGAAACATGAGAGTCCAGAGCTCCCTCCTCCTGGTAGTCCTCCTGGCTTTGGAGACT CAGTTGCCTGTTGCCTTGTGTAGAAAGAAGGGAGACAAATTGGGGGGTTGCCCACCAGACGATGGACTCT GCAACCAGAATACTCCTCCTGATCAGTGCTTGAATGACAAACAGTGTCCCTCTTCTTGGAAGTGCTGTCG CCGAGCCTGCTTCCTCCAGTGTATGCCTCGTGTCTTTGTAAAGTCGGGCAAATGCCCCGTGGATCAACTT CGCTGCCTCAGTCCCACAAAGCACATGTGTAACAAAGACTCGGACTGCTCAGGCAAGAAGCGGTGCTGTG CCAGCGCCTGTGGCCGGGACTGCCGAGACCCCAGCAAAAGTACCATTTGAGACCCCAGCAAAGGCTAATT CTGGCTTACGATCTATGGCTCTGCACCCCAGGTGTGGTTCCCACCAGAAAGAGATCATGATGTGCCAGCC AGGGATCCTGCGACCCAGATCTTCTCTCCAGAGACAGTTGTGACCTTCTGACAAGCACTGGCCTGTTGTC AGCTCTCTTACAACTCATGCCTCCTGCATTCTCCTCTGAGTGCCAAGGTCCACACCAGACATCAAAAGCA TAGCTCATCATTCGGCTCCGGCACCCCACACCACTGTTGTGCTGGGCTTCCCATCCCACCTCAGAACCAG TGTTATATCTCTCTGTACATTGTTCCCTCCACTTCAAATGCCAAGCCAGGCAGCCCCTTCTCTCCCGAGC CCTGAACTCACCTGCAGACTATCCTCCCCAAAATAAGGTGTGTCCAGAAAGAATTTTCCAAACATCAGTC CATCCACACATGCTGCCTCAGTCCCCATCATCCCTGCAGAACCACCCCGTAATGTGTAAAGTTGTGTGAA GTACCTCTGTACATCTACTCACTTAACGTCTTCATTTAAACGGCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3987617 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3987617 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003779 same experiment
	EMAGE:31268 same embryo
	EMAGE:29425 same embryo
	EMAGE:31227 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source