EMAGE mouse gene expression spatial database

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EMAGE:31227

Ssh3 ( MGI:2683546)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003754_01	euxassay_003754_02	euxassay_003754_03	euxassay_003754_04	euxassay_003754_05

euxassay_003754_06	euxassay_003754_07	euxassay_003754_08	euxassay_003754_09	euxassay_003754_10

euxassay_003754_11	euxassay_003754_12	euxassay_003754_13	euxassay_003754_14	euxassay_003754_15

euxassay_003754_16	euxassay_003754_17	euxassay_003754_18	euxassay_003754_19	euxassay_003754_20

euxassay_003754_21	euxassay_003754_22	euxassay_003754_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 10

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 weak expression: see section 01 02

rectum

moderate

regional

moderate expression: see section 11

urethra of male

strong

regional

strong expression: see section 11

bladder

strong

regional

strong expression: see section 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6969
Entity Detected:	Ssh3, ( MGI:2683546)
Sequence:	sense strand is shown >T6969 CTTCCTGGTTGTGCGAAGGCAGGGACTGACCGTAGGGCTGTGGGAGGGGTCGTGCCGGGTGTTCGCCCAG GTGTTCCGGGCCTGCCTACCTTATGGCGCTGGTCACAGTGAGTCGCTCGCCGCCGGCAAGTGGCCACTCC ACGCCTGTGGGACCCACGCAGGACCGAGTGGTAAGACGCAGAGGCCGGCTCCAGCGCAGGCAGAGTTTTG CGGTGCTCCGAGGGGCTGTCCTGGGACTTCAGGATGGAGGGGACAGTAATGTTGCATCTGAGGCTGACTC TGAGCCAATGGAGGAACCCTCAGGTGAGGAACAGCCCACCGAGGACCAGACTGACAAAGGGCAAGGACTC CAGAGTCCCTGGAAACAAGTGCAAAAGCGGCACCTGCACCTCATGGTGGAGCTACTGAGGCCGCAGGATG ATATCCGCCTGGCAGCCCAGCTGGAGGCAGCCCGACCCCCCAGACTTCGCTACCTGCTGGTAGTATCCAC GGGAGAAGAACTGAGTGAGGAGGCCATCCTGCTTGGGGTGGACTTCCCTGACAGCAGTTCCCACAGCTGC ACCCTGGGCCTGGTCTTGCCCCTCTGGAGTGATACCCAGGTGTACCTGGACGGCGATGGGGGCTTCAGTG TGACATCTGGTGGTCAAAGCCGAATCTTCAAGCCTGTCTCTATCCAGACCATGTGGGCCACACTGCAGGT ATTGCACCAGGCATGTGAGGTAGCTCTAGGCAGTGGCCTTGTGCCTGGTGGCAGTGCCCTTGCCTGGGCT ACTTACTACCAGGAGAAACTCAACTCTGACCAGGGTTGCCTCAATGAGTGGATGGCCATGTCTGACCTGG AGTCCTTTCGACCACCTAATGCTGAGCCTGGACAGGCTTCAGAGCAGGAAAAGATGGAGCAGGCGATCCT GGCTGAATTGTGGCAGGTGTTGGACACCAGTGACCTGGACAGCGTCACTTCTAAAGAGATCCGTCAGGCC CTGGAGCTGCGTCTGGGATGTCCTCTCCAGCAGTACCGTGACTTTATTGACAACCAGATGTTGCTGCTCA TGGCCCAGCAAGACCGGGCCTCTCGCATCTTCCCCCACCTCTACTTGGGCTCTGAGTGGAATGCTGCCAA CCTGGAGGAACTTCAGAAAAACAGAGTAAGTCACATCCTGAACATGGCCCGGGAGATTGACAACTTCTTC CCTGAGCGCTTCACCTATTACAATGTACGTGTCTGGGATGAGGAATCGGCACAGCTTCTGCCCCACTGGA AGGAGACACATCGATTCATTGAGGATGCCAGAGCACAGGGCACTCGGGTGCTAGTCCACTGTAAGATGGG TGTCAGCCGTTCTGCCGCCACGGTACTAGCCTATGCCATGAAACAGTATGGCTGGGACCTGGAGCAAGCC CTGATCCACGTGCAGGAGCTCCGGCCCATTGTACGCCCCAACCACGGCTTCCTGCGCCAGCTACGGACCT ACCAGGGCATTCTGACTGCCAGCCGGCAGAGCCACGTCTGGGAGCAGAAAGTGGGTGTGGTCTCCCCAGA GGAGCCCCTGGCGCCTGAAGTTTCTACACCATTGCCACCTCTTCCACCAGAACCAGGGGGCAGTGGGGAG GTGATGGTAATGGGCCTGGAAGGAAGCCAGGAAACCCCAAAAGAAGAACTTGGACTGCGGCCCCGCATCA ACCTTCGAGGGGTCATGCGCTCCATCAGTCTCCTGGAGCCCTCAGAGTCAGAGAGTACCCCAGAGGCGGG AGGCCTGCCAGAAGTTTTCTCTTCAGATGAAGAGCCTCTTCATCCATTCTCTCAGCTTTCAAGAGCCAAA GGTGGCCAGCGGGTCCGCAAAGGGCCTTGGCCTGCCCTGAAGTCTCGACAGTCTGTGGTTGCCCTTCATA GTGCTGCCCTGGTGGCCAGCAGGACACGGGCCTTCCAAGAACAGGGGCAAGGGCAAGAGCAGAGTGAGCC TGGTATGTCTTCTACCCCTAGGCTCCGGAAGGTGATGAGGCAGGCCAGTGTAGATGACAGCAGGGAGGAG GACAAGGCCTAAGCCCTCATGCTGTGTCCGCCCCCAGACACTAATCGCGACTGGTCACAGCGCCTCAGAT GAGTGGTCTTTACACCTATGGGCCTGCACACGCCCTGCAGCTCCTTCCCAGCTGCCCTCAGACCTTTGCC ACTTCCTGCAGCCTTAGGTCTCAGCCCATATCTACCTGTTAAGGGCTCAATCCTTTTTCTACATAGGGTC TGGGTTAGAGCCGTTTAAGGTGCCTTTAGGGTAATAACACCCTAGTTTCATTGTACACTCACTCACTTAA CATTGTGTACAGCCATGGAGTTAAAACCAGAATTTTTTAGGGTGGGTTTTCTAGTTCATGTCTCCCTTGC CCTCGACTTGTGGCCATTCCACCCTGCGTCTTCCACCCTCTCCTACTGCAGTCCTGGAGACAGCTAGGCC AGGGGACTAGTGGCCACAAAGGCAGATAGCACTTTCAGTCCCCAGTCCCACAGGCTCTGAGGGGGCTCAC AGGTCACCCACCCACCCAACTGACCCGAATCTCTGCTGCTGCCCTCAGTTCCCCCATGCCCCTCACTGGG TCACAGCACTGAGAGTCTTCTTGTTCTTGAAGATCTCTGGCCAGGCAAGTTTTTCATAGTTGTATAGTAT TTGGCTTTGTATTGAGAAATAAAAGACATTTTCATATTAAAAGACATTTTCCATTTGTGACAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3987714 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3987714 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003754 same experiment
	EMAGE:31268 same embryo
	EMAGE:29400 same embryo
	EMAGE:29425 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source