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EMAGE:29561

Cryge ( MGI:88525)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29561
euxassay_006853_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9122
Entity Detected:Cryge, ( MGI:88525)
Sequence:sense strand is shown

>T9122
CCCATCCGACCTGCCAACACCAGCCATGGGGAAGATCACCTTCTATGAGGACCGCGGCTTCCAGGGCCGC
CACTATGAGTGCAGCACCGACCACTCCAACCTGCAGCCCTACTTCAGCCGCTGCAACTCTGTGCGCGTGG
ACAGTGGCTGCTGGATGCTCTATGAGCAGCCCAACTTCGCAGGCTGCCAGTACTTCCTGCGTCGCGGGGA
CTACCCTGACTACCAGCAGTGGATGGGTTTCAGTGACTCTGTCCGCTCCTGCCGCCTCATCCCCCACTCC
AGTTCTCACAGGATCAAGATCTACGAGCGAGAGGACTACAGAGGCCAAATGGTGGAGATCACAGACGACT
GCTCCCACCTGCAGGACCGCTTCCACTTCAGTGACTTCCACTCCTTCCACGTGATGGAGGGCTACTGGGT
CCTCTACGAGATGCCCAACTACCGGGGGCGGCAGTACCTGCTCAGGCCTGGGGAGTACAGGCGCTACCAC
GACTGGGGCGCCATGAATGCCAGGGTGGGCTCTCTGAGGAGAATCATGGATTTCTATTGAAATATTTTTA
CTCTACCATTTTCTCCATCTGGACAGTAATAAAATATTTCCTGTGTGTTTCATGCAAAAAAAAAAAAAAA
AAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6824205 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6824205 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006853 same experiment
 EMAGE:29560 same embryo
 EMAGE:31175 same embryo
 EMAGE:31158 same embryo
 EMAGE:32002 same embryo
 EMAGE:32004 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS