EMAGE mouse gene expression spatial database

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EMAGE:31175

AU040829 ( MGI:2144423)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006838_01	euxassay_006838_02	euxassay_006838_03	euxassay_006838_04	euxassay_006838_05

euxassay_006838_06	euxassay_006838_07	euxassay_006838_08	euxassay_006838_09	euxassay_006838_10

euxassay_006838_11	euxassay_006838_12	euxassay_006838_13	euxassay_006838_14	euxassay_006838_15

euxassay_006838_16	euxassay_006838_17	euxassay_006838_18	euxassay_006838_19	euxassay_006838_20

euxassay_006838_21	euxassay_006838_22	euxassay_006838_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 15 weak expression: see section 16

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20 21

neural retina

weak

regional

weak expression: see section 01 02 22 23

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 14 15 16 17 18 19 weak expression: see section 02 03 04 13 20 21 22

trachea

moderate

regional

moderate expression: see section 13 14 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 15 weak expression: see section 16 17 18

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

right lung

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 09 10 11 14 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

left lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9134
Entity Detected:	AU040829, ( MGI:2144423)
Sequence:	sense strand is shown >T9134 CGGGGACCACCCGGCCAGCTGGCTAGCCAGCCTGCGGAGACTCCCCGGTTCGCTCGCCGGGCTGGGGCTG GGGGCTAGCTACAGCTGGGGCTGACCGCCGGGCGCCTTGGGCGGCCCCGGGCGTGGCACAGAGAAGGATG CGGCGGGCGGACTAGGCGGAGGTGCTCTCGGGAGGCTGGGCGCGGGCCAGGCGGCTCCCCAGGCTCCGCA GTGCCGCCGCCGTCGCCCGGGAGGCTGGGCGCGGGAGCCATGTAACCCTGCGGTGGGCTCCGGGCAGCTC AGTCCTTCCCCAGCTCCCGGGCTAGCGCGGCAGCGGGGCCACGATGAAGAAGCAGTTTAACCGCATGCGC CAGTTGGCCAACCAGACGGTGGGCAGGGCAGAAAAGACTGAAGTTCTGAGCGAAGACCTTCTCCAGGTGG AGAAGCGCCTAGAGCTGGTGAAGCAGGTGTCCCACAGCACACACAAGAAGCTCACCGCATGTCTGCAGGG TCAGCAGGGAGCAGAGGCCGACAAGCGATCAAAAAAGTTGCCTCTGACGACTCTGGCGCAGTGCTTGGTG GAGGGGTCCGCGATCCTGGGCGATGACACGCTTCTTGGGAAGATGCTGAAGCTCTGCGGAGAGACGGAGG ACAAGCTAGCTCAGGAGCTGATCCACTTTGAGCTGCAAGTGGAGAGGGATGTGATCGAGCCCCTGTTCCT GCTCGCTGAGGTGGAAATTCCAAATATTCAAAAGCAGAGGAAACATTTAGCCAAGCTGGTGCTTGACATG GACTCTTCCCGGACCAGGTGGCAGCAGACTTCCAAGTCTTCAGGGCTTTCCAGCAGCCTTCAGCCTGCGG GTGCCAAAGCCGATGCCCTCAGGGAGGAGATGGAGGAGGCTGCCAACAGAGTGGAGATTTGCCGGGACCA GCTTTCTGCTGACATGTACAGTTTTGTGGCCAAAGAAATTGACTATGCAAACTACTTTCAAACGCTAATA GAAGTGCAAGCTGAGTACCACAGGAAGTCGCTGACACTGCTGCAGGCTGTGTTGCCTCAGATCAAAGCCC AGCAGGAGGCCTGGGTGGAGAAGCCTTCCTTTGGGAAGCCCCTGGAGGAGCACCTGATGATCAGTGGCCG AGAGATCGCCTTCCCTATCGAGGCGTGTGTGACCATGCTGCTGGAGTGTGGGATGCAGGAGGAGGGCCTC TTCCGGGTAGCCCCCTCAGCCTCCAAGCTGAAGAAGCTGAAAGCTGCTCTGGACTGCTGTGTGGTGGATG TCCAGGAGTACTCGGCTGACCCCCACGCCATAGCAGGGGCTTTGAAGTCTTATCTCCGAGAGTTGCCAGA GCCTCTTATGACCTTTGAACTCTATGACGAATGGATCCAGGCTTCTAATATCCAGGAGCAAGACAAGAGA CTTCAGGCTCTCTGGAATGCTTGTGAGAAGTTGCCCAAGGCCAACCATAACAACATCAAATACTTGATCA AATTTTTATCGAAACTGTCTGAATATCAGGATGTAAACAAGATGACTCCCAGTAACATGGCAATTGTCCT GGGACCCAATCTCCTTTGGCCACAGTCAGAGGGGAACATCACCGAGATGATGACCACAGTTTCCCTGCAG ATCGTTGGCATCATCGAGCCCATTATCCAGCACGCAGACTGGTTCTTCCCTGGAGAGATAGAGTTCAACC TCACAGGCAGTTACGGGAGTCCAGTCCACGTGAACCACAATGCCAACTACAGCTCAATGCCCTCCCCAGA CATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGGCCCCTCAGTGTTGCCACAGACAACATG ATGCTGGAGTTTTACAAGAAGGATGGCATGGGTGTGAGGGTCATGGACACATCTTGGGTGGCACGAAGAG GCTCCTCTGCTGGTCGGAAAGCATCCTGCGCCCCGCCTTCCATGCAGCCTCCTGCCCCACCCTCCGAGCT GGCTGCACCCTTGCCTTCACCCCTGCCGGAGCAGGTCCCAGACAGCCCTGCCACTCCAGCTCCTGCGCTC TCTCCATCAGGTGCCAGCCTGCAGCCCACCCCCGAGCGCCCCAGTGTGTCTAAAAGCAAGGAACTGTCGC CAGGGTCTGGGCAGAAAGGAAGTCCAGGTTCCATCCAGGGGACACCCTGCCCAGGGACGCAACTGGGGCC ACAGCCGGCTGCCAGCCCCAGCCAGCTTCCTGCAGACCAGAGTCCTCACACCCTCCGGAAAGTTTCAAAG AAGGTGGCACCAATTCCTCCCAAGGTCCCCTTCGTGCAGCCAGGGACTGTTTCAGATCAGCCCGTTGGCC AGCCATCCCCAGTCAGCCTGTCTCCTACTCCACCAAGCACCCCATCCCCCTATGGACTGAGCTACCCTCC AGGCTACTCCATGGCCTCTGGCCAGCTTTCTCCTGCCTCAGCTCCTCCCCTGGCCTCTCCCTCTGTCTTT ACAAGCACTTTAGCCAAATCTCGGCCCACCCCTAAGCCTCGACAGAGGCCCACCCTGCCGCCTCCTCAGC CTCCCAGTGTCAGCCTGTCGGCTTCAAGTCCACAGTCCACAGAGCACCCCATGTTAGACGGCATGTCGCC GGGGGAAAGCATGTCTACAGCTGTGTGACATGAGTGGCGCAGTGTTGGATACGTATGAGGGGGATTTTCA GGATCTCGTCCACTTTGATGTCCCCTCCATCCACATAGAGCTGGGGTCAACGCTCCGCCTGAGCCCCCTG GAACATGCACGGCGGCACTCGGCAACAGACAAGAGGGACTCGGAAGAGGAGTCTGAGAGTACAGCCCTTT GATGTGGCCCAACCTACACGTGTACATACGTCTGTGGGCCCTGAGACACCACCCAGGGCAGTGCTGGCTA GCCTGCCAGGGACTTGCCATCAGCTCTTTGCTGTCATGTCCAACACCAGGGTGGAGTTGGGCGGAGGAAA AGAGTTCTCCTGTCCATGTGGCCAGTGTTGGTGGTGCAGATTCTGTTTGTTCAGTCTGGGTGATGATTCC AGCTTTTTTTTTTTTTTTTTACCCTTTGCCTTTTGACTTTGTGCCTATTTTGATCCATCTTTGGCCTCTA TGGCAAGCAGCATCCAACTCTCCACTCAAGGGCTTGTCAGGATGGCCTTTGCAGGATGAGGGTGCAGAAG AGTCCCCTTACTGCCTTGACTGGGACATCAGGTCCTACAGCATCATCATAAGGGCCAGAGAAGAAGCAAG ACAGGAGGAGACCCTTTCACTAGGGCAAGAGATGTCCTGGGGCCAACCAGAAAAACCTTTCAGGCCACCC TTAGTCACAGCTCCAAAGTACTCAGCTTCAGACATCCACTTCTGACAGCTTTACTTTCCCCGTGTGATGA CGGCTTCCTCTACTCCACGCTGACAATCTGAACTGAGGATTTGAACATAAGTGACCCTACGTAGGTCTCC ATGTTCCCAAACACATTAGGGATCTTAGAGCACTTTCGCATTCAGGCACCCCGCTTAGTAGGTGTGTGGG GCCTGACTCTCCTCTTCTGGAGGTTACAGCTGACTAGCAATAATTCTGTTTCCCAAAGCAGGTGGAGAGG GTGGTACTGGGCACCTAGCCCACAGCGTGGCCTGCGTGGCTCACCAGCAGACCCTGCTCATCCTTCTCAC CACGTCCCATGCAGCCCTGGGGCAAGGGCGCAGGAAGCCTGTTCATACTTTGGGATTAAGGGAATAAAAC AGATCTGGGCCATGCATGCACAGTCAGTTTAAAGTTTGTTCCTCTGAAAATAGATATACCTATACATGAT ATATTTGTATATATGAAATCTATATTTGTTTAATTTGAGCCATTCAATCTAAACCTATGTACAGGTGTAC AATGGAAAATTCTAAATGCTTAGTTATTTTTCCCAGCAGTGTGAACTCACCCGTCTCTGAGAGTGGGATC TGCAGACTCGACCTTACAGCTTTGCTCACTTCCTGGCAAGGGCAGTCCAGTCCCCAAATTTGTAACGGAG CCTGGGGGTTGAAGGTTATCTTTACATATATGTACAAACTGTTGTCAAGAGTAATGTTATTAAAATAGAT TTATTAACCCTGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825221 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825221 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006838 same experiment
	EMAGE:29560 same embryo
	EMAGE:29561 same embryo
	EMAGE:31158 same embryo
	EMAGE:32002 same embryo
	EMAGE:32004 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source