EMAGE mouse gene expression spatial database

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EMAGE:31158

Pcdhga7 ( MGI:1935219)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006823_01	euxassay_006823_02	euxassay_006823_03	euxassay_006823_04	euxassay_006823_05

euxassay_006823_06	euxassay_006823_07	euxassay_006823_08	euxassay_006823_09	euxassay_006823_10

euxassay_006823_11	euxassay_006823_12	euxassay_006823_13	euxassay_006823_14	euxassay_006823_15

euxassay_006823_16	euxassay_006823_17	euxassay_006823_18	euxassay_006823_19	euxassay_006823_20

euxassay_006823_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9130
Entity Detected:	Pcdhga7, ( MGI:1935219)
Sequence:	sense strand is shown >T9130 CTGGATTCCCGGCTTCCCTTCCGCACACTGGGGTCCTGGCGGACACAACTGGGTTCTGAGGCACCTTCCA AGACCTCTCAGGGTGTGAGCGAGATCCTCAGGGCAGCAATGGCGCCTGGACAGAGAGGCTGGGGCTCGCA CTACAGAGGATTTGTGCTGCTCTCCATTCTCCTGGGGATGCGGTGGGAAGCCTGGGCAGGACAGATTCTC TACTCCGTGTCTGAAGAGACAGACAAGGGATCTTTTGTGGGAAATATCACCAAGGATCTGGGACTGCAGC CTCGGGAGCTGGCGGAGCGCGGGGTCCGCATCATCACCAAAGGTAAGACCCAGCTTTTCTCTCTGAACCC GCGGAGCGGCAGCCTGGTCACCGCGGGCAGGATAGATCGGGAGGAGCTGTGCGCTCAGAGCCCGCGATGT CTCGTGAGCTTTAACATCTTGATAGAAGACAAGATGAATCTTTACCCTATAGAGGTGGAAATAATGGATG TTAATGACAACGCTCCCAGATTCTTAACAGAAGAAATAAACATAAAAATTATGGAGAATGCAGCTCCTGG GGTGCGGTTTCCACTAAAGGAAGCTACAGATTCAGACGTGGGCAAGAACTCCCTCCAGAGATACCAGCTC AGCCCCAATCACCATTTCTCCCTGGTTGTGCAAAGTGGAGATGATGGCACCAAGTACCCAGAACTGGTGC TGGAGAAGGCTCTTGATCGGGAAGAAGAGGGGCTTCACCATCTGGTCCTCACAGCCTATGATGGCGGGGA CCCGCTCCGATCTGGCACTGCCAGCATCCGCGTGACAGTGGTGGATGTGAATGATCACACGCCAGTCTTC TCTCTACCCCTGTATCAAGCGACAGTCCCTGAGAACGTGCCAGTAGGCACAAGACTTCTCACTGTAAATG CTATCGACTTGGATGAGGGAATCTATGGGGAAGTGACTTATTCTTTCTGGAAAATAACTCCAGAACTTCT ACAAATATTCCATCTGAGCTCCCTGACAGGAGAGCTGTCGACTTTAAAAGGTCTAGATTATGAGCAATCC AGCTTCTATGAGCTGGAAGTTCAAGCTCAAGATGGTGCTGGCAGTCTGACCAAGGCAAAAGTACTGATCA CAGTTTTGGATGTGAATGACAATGCCCCAGAAGTGACTGTCACATCTGTGAGCAGCCCAGTCCCTGAAGA CACGCCACCTGGGACAGTGATCGCTCTCTTCTACCTCCAAGACAAAGATTCGGGGAAGAATGGTGAGGTG ACCTGCTCTTTGCCAGAAAACCTGCCTTTTAAGTTAGAGCGATCAATTGATAATTATTACAGGTTGGTCA CAGCACGAAACCTGGACCGGGAAAAGATGTCTGTGTACAACATCACACTGAAGGCCACAGATGGTGGAAT CCCTCCCCTGTCCACTGAAACTCGCATGTCCATTCATGTGTCAGACACCAACGACAACCCACCCACCTTT TCCGACTCTTCTTACTCTATCTACGTCCCGGAGAACAATCCCAGAGGAGCCTCCATCTTCTCGGTGACTG CACAAGACCCGGACAGTGACAAGAATGCCCAAGTCACTTACGCTTTAGCAGAAGACACCATCCAGGGGGT ACCGGTGTCGTCCTACGTCTCCATCAACTCTGACACGGGTATCCTATATGCACTTGGATCCTTCGACTAT GAGCAATTTCGGGATCTACAACTTCGAGTGACTGCTCGTGACAGTGGGGACCCACCTCTCAGCAGTAATG TGTCACTGAAACTCTTTGTGCTGGACAAGAATGATAACGCCCCTGAGATCCTGTACCCTGCCCTGCCCAC TGATGGCTCCACAGGGGTGGAGCTGGCTCCCCGCTCTGCAGAACCTGGATACCTAGTGACCAAGGTGGTA GCTGTGGACAAAGACTCAGGCCCAAATGCTTGGCTGTCCTACCGCCTGCTTAAAGCCAGTGAGCCAGGGC TTTTCTCAGTAGGGCTACACACTGGTGAGGTGCGCACAGCAAGAGCCCTGCTGGACAGAGATGCGCTGAA ACAGAACCTTGTGGTGGTTGTACAGGACCATGGCCAACCCCCACTCTCAGCCACTGTGACACTCACTGTG GCTGTGGCCAACAGCATTCCTGATGTCCTAGCAGACCTAGGCAGCATTAGGACCCCCAGTGAACCTGATG ATTCAGATATCACCCTCTACCTGGTGGTGGCAGTGGCCACTGTTTCCTGTGTTTTCCTTGTCTTTGTCAC TGTGTTATTGGCTCTCAGGCTGTGGCGTTGGCACAAATCACACATGCTACAGGCTTCTTCAGGTGGTGGC CTGGAAGATGTGCCTGCCTCACACTTTGTAGGTTTGGACGGTGTCCAGGCTTTCCTACAAACATATTCCC ATGAGGTTTCACTCACTGCAGACTCTCGAAAAAGCCACATTATCTTCCCTCAGCCCAATTACGTGGATAC ACTTATCAGCCAAGATAGCTGTGAGAAAAGTGATTCTTTGTTAACATCCATAGATTTTCAAGAATGTAAG GGTGAAGCCCCAAGTTCCCAGCAAGCCCCGCCCAACACTGACTGGCGTTTCTCTCAAGCCCAGAGACCCG GCACGAGCGGATCCCAAAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTTGATACAGAGATGCT GCAAGCCATGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGGGAGGGGGCGCTGGC ACCATGGGTCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGACTACCGCCAGAACG TGTACATCCCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAAACGAGATGGCAAGGCTCCAGCAGG TGGTAATGGCAACAAGAAGAAATCGGGCAAGAAAGAGAAGAAGTAATATGGAGGCCAGGCCTTGAACCAC AAGGCAGCCTCCCTCCCCAGCCAGTCCAGCTCGTCCTTACTTGTACCCAGGCCTCAGAATTTCAGGGCTC ACCCCAGGATACTGGTAGGAGCCAGGGCCATGCTCCCCGTTGGGAAACAGAAACACGTGCCCAAGCCAAC ACCCCCTCTCTATACCCTAGGGAGTTGAATATGCAAAGAGAGTTCTGCTGGGACCCCCCTATTCAATCAG TGATTGTACCCACATGGGTAGCAGGGTTTGTGTGTGTGGACATACACACACACACACACACACACACACA CAAACCATGCCACACTCCCTTAGTTACAGCTGAACTCCTCCATCTTCCAACCCAGGCAGGCTTATCCATC TCCTGCCGCCCTCTCCCCACCCCACCCACTCCTTCAGTCCCTCTCTTTGAGCAAGGTAGCTGGGCTGTTG GGGTGACCGGTGAGCTAAGCCGGCTCCTAGTTCTGAACGGTAGGGAGAGTATTACAATCTTTTGGCCTCT CCTTCAGTTCAGCTTTCCCCCAAAGCATGGTTTGGTACCAGTCTTCTCTTTTCCTTCCAGAGCCTGAGAC CAATGCTTAAGTTTTGGGGGAACAGGCACCCTCCCTCTGGTACTGATGATGCTTGCTGGATTTTGGGAAG GCATTTTGCTACTAAGCCTCTTTTCAATGCCTGGGGACAACTAGTCTTTTGTTTTTCATTGTTTGATGTT CCCACTGCATGCTGTGACTTCCCTACACCTCAAAGAAGAGACTCCTTTGCATGTCTGGAGACAGTAGGGG GTGGTAAACTAAAGGGAAGTTGAGAGTGTATACTGCTGGGAGTGGGGCAGTGGACAAAACAGCCTGTTAA TAGGGTCTTGCAAGGGGCTCTGTATGTACCCAGGGGACTGGCTCAATCCTCACATTCTAGCCATAGACAA ACACCAGGCCAGACCCCTCCATTCTGGTTCAGCCTGGGCAGCTTGGGCTGAGCCACCAGGACCAATGGAT TTAAGCTGACATTTCAGTCCAAGACGACGACTTCTAAGTGAGTTTAAGACCAGAGAGGAAAGAGGGGCCT CTGTGGGTGCTGGGTACTCCAGAGGTGCCCTTGGTGGGAGGACCAGTGGTCTTAGCAGGAAGGGGGGCCC AGCAAGGTCATTCTTGGACCCTGGGTCTAGTCCAGTAGCTAGAAAAAGGGACCAAGTGGCCATAAAGTCC CAGCCAATGATGGGGCTTTTCCAGTGGGCCCCTGTAGACCTCAAGCCCCTGGCCTCCACCTTACCAGGTG CCATTTCTTCTCAGAAGGCCACTGCCCAGGCCCCCAGGCCGCCCCCTTGTGGCCAGAGAACGGTTAAAGC CCCCCAGTGCCTCCTTGTGCATAGAACTTCCTCTGCCCTAGCCCTTACAGTAGTGTAGAAGATCCCCCTC CCCCGCTGGCGTAGAATAGCCAATAGTATAGTGTGGTGTGCTTTTACGTGATGGCGAGTGGGCAGCGGGC GGTGGGCTGTACACAGCCGTCTGTCCTTTGAATCTCAATCTGCCTGCGCGGCCCGTGCTGTGTTTTGTGC TGTGTCCACACGCTGCGGCGACCCCTCCCTGTACTGACTTCTCTATAAAAAGCTTCTCTTCGCATAGTCA CGTAGCTCCTGCCCATCCCCCTTCCGATATCTCTCGCAAGTTTTATACTCTAATATTTATATGGCTTTTT TCTTCGAAAATTAAAAATAAAAAAATGGTTTCTTCTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825399 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825399 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006823 same experiment
	EMAGE:29560 same embryo
	EMAGE:31175 same embryo
	EMAGE:29561 same embryo
	EMAGE:32002 same embryo
	EMAGE:32004 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source