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EMAGE:29708

Freq ( MGI:109166)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29708 EMAGE:29708 EMAGE:29708 EMAGE:29708 EMAGE:29708
euxassay_000408_21 euxassay_000408_01 euxassay_000408_02 euxassay_000408_03 euxassay_000408_04
EMAGE:29708 EMAGE:29708 EMAGE:29708 EMAGE:29708 EMAGE:29708
euxassay_000408_05 euxassay_000408_06 euxassay_000408_07 euxassay_000408_08 euxassay_000408_09
EMAGE:29708 EMAGE:29708 EMAGE:29708 EMAGE:29708 EMAGE:29708
euxassay_000408_10 euxassay_000408_11 euxassay_000408_12 euxassay_000408_13 euxassay_000408_14
EMAGE:29708 EMAGE:29708 EMAGE:29708 EMAGE:29708 EMAGE:29708
euxassay_000408_15 euxassay_000408_16 euxassay_000408_17 euxassay_000408_18 euxassay_000408_19
EMAGE:29708 EMAGE:29708 EMAGE:29708
euxassay_000408_20 euxassay_000408_22 euxassay_000408_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3852
Entity Detected:Freq, ( MGI:109166)
Sequence:sense strand is shown

>T3852
TGGCCTCGAGGCAGATTCGGACGAGGGCGGCTGGCTTTGTGGAGATGAGGAAGGCTGGGTGGGCACAGGG
GCGGTGTCAGGCTGCGGTGGCCTCATGGCACAGAAAGAGGCTAGTGGTTCCCACACATCACAGCTCTGGG
CCTGGACTATACCAGCCCATCGTAGAGGGACAGCGCCTGCACAATGGAGGGATCAGCCTTGGAGCCTTCC
TGGAACTCCTGAAGAGTCAGCTTCCCATCAGCATTCTTGTCCATCATGGCAAAGATCCGGTCCACCCTCT
TCTCTGGTGTGTTCTCTTCTTCTGGGAGCTCCACGGTGTTCCCCACCATCTGGTAAATGGCGTCCACTAT
GTCGAGCATCTCGTTCCTGGTGATGTAGCCATCGTTATCCAAGTCATAAAGCTTGAAGGCCCATCGCAGC
TTTTCATCCAGGGTCCCCCTTGAGGTCACCGACAGTGCCTGGATGAACTCGGAGAACTCAATCCTGCCAT
CCTTGTTCTCGTCGAAGACGTTGAAAACAAACGTGGCGAACTTGGTGGGGTCTCCAAATGGGAAGAACTG
C
Notes:The probe template was PCR amplified from IMAGE:423183 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:423183 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000408 same experiment
 EMAGE:31958 same embryo
 EMAGE:29703 same embryo
 EMAGE:29780 same embryo
 EMAGE:29840 same embryo
 EMAGE:31998 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS