EMAGE mouse gene expression spatial database

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EMAGE:31958

Hoxb5 homeobox B5 ( MGI:96186)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000381_01	euxassay_000381_02	euxassay_000381_03	euxassay_000381_04	euxassay_000381_05

euxassay_000381_06	euxassay_000381_07	euxassay_000381_08	euxassay_000381_09	euxassay_000381_10

euxassay_000381_11	euxassay_000381_12	euxassay_000381_13	euxassay_000381_14	euxassay_000381_15

euxassay_000381_16	euxassay_000381_17	euxassay_000381_18	euxassay_000381_19	euxassay_000381_20

euxassay_000381_21	euxassay_000381_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity

weak

regional

weak expression: see section 12

pectoral girdle and thoracic body wall skeleton

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16

nasal capsule

weak

regional

weak expression: see section 08 09 10 11 13 14 15 16 17 18 19 20

axial skeleton

weak

regional

weak expression: see section 08 09 11 12 13 14 15 16

dorsal grey horn

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3844
Entity Detected:	Hoxb5, homeobox B5 ( MGI:96186)
Sequence:	sense strand is shown >T3844 TTTGAGTTTCTGCTGGAAACTTCCATAAGGGGCAGCAGTTGAGGTTGGGTAGTGCTGGGCCCAGCTGAGC TGGCCTGGGAAATGGAGCCCACTGTCTGTGTCTCTTTTCTCCCACCTCATCCTTCTTCAGCCCCACCCAG CCCCCACTCCCTAGGCTCAGGTAGCTTGTTCCTTGGGGTGGGAAGGGAGCTAGGGAAGGGTCAAAGTGTG GACATTGAGAAGAGGAGAGGAAAGGAGCAAGAGCTGAACTCCTGCTGCCTGGTAGGCCCCACAAGGCCTA GTCTGGAAGCGTATGGAATCAGAAATAATCCTCAGTGTAAAATGTCTTGTGATTTTTCTCTGTGAATCCG TGGGTCTGGCTAGAAGGCCCAATGCTGTAAATATGGGGATAGTCTGGGTCAGGCCAATCACTTCCTCTCT CACCCATTTCGCTTCCAAGACCATTTGTAGTGAGCGGGTGGATGCTGTGCTACGTGTGAAATCTGTCTTT GCCAGGCCTGTCTCAGTGATTAGCTTTTGGTATGTCTGTAGCTTT
Notes:	The probe template was PCR amplified from IMAGE:421728 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:421728 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000381 same experiment
	EMAGE:29703 same embryo
	EMAGE:29780 same embryo
	EMAGE:29708 same embryo
	EMAGE:29840 same embryo
	EMAGE:31998 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source