Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29862

Celf1 CUGBP, Elav-like family member 1 ( MGI:1342295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29862 EMAGE:29862 EMAGE:29862 EMAGE:29862 EMAGE:29862
euxassay_002184_01 euxassay_002184_02 euxassay_002184_03 euxassay_002184_04 euxassay_002184_05
EMAGE:29862 EMAGE:29862 EMAGE:29862 EMAGE:29862 EMAGE:29862
euxassay_002184_06 euxassay_002184_07 euxassay_002184_08 euxassay_002184_09 euxassay_002184_10
EMAGE:29862 EMAGE:29862 EMAGE:29862 EMAGE:29862 EMAGE:29862
euxassay_002184_11 euxassay_002184_12 euxassay_002184_13 euxassay_002184_14 euxassay_002184_15
EMAGE:29862 EMAGE:29862 EMAGE:29862 EMAGE:29862 EMAGE:29862
euxassay_002184_16 euxassay_002184_17 euxassay_002184_18 euxassay_002184_19 euxassay_002184_20
EMAGE:29862
euxassay_002184_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6065
Entity Detected:Celf1, CUGBP, Elav-like family member 1 ( MGI:1342295)
Sequence:sense strand is shown

>T6065
CGGACGCGTGGGCGGACGCGTGGGTCGACCCACGCGTCCGATTTTGGAGACAAACCTGTGTGAGTTTTTT
ATTGGTACAAACGTTGTATTTAACACTAGGGGTTTTGTACAGTTTTTTGCCTTTTCTACTAGAAAACAAT
GTAAAGTGATTTCACAATGTGAAGAGAAAAAAATTGCCACTATGACCAAACGCACAGTCTGTTCTGCAGC
AACAACGGGATTCAATCAACTCAAAGTCGTGATTCAGCCGTAGAAATGCTTTTCTTGACCTTGTTTGAGC
TTTCCTTTTTTCCTGTTTGATTTATAAAAGATGTCTTTTGTGTGTGAACTTGTGTTGTACTCTGTAGAAA
ATTATGGATTTTGCTTTAATGGTTTTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3490700 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490700 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002184 same experiment
 EMAGE:32093 same embryo
 EMAGE:31196 same embryo
 EMAGE:30212 same embryo
 EMAGE:32114 same embryo
 EMAGE:31666 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS