EMAGE mouse gene expression spatial database

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EMAGE:31196

Nxph3 ( MGI:1336188)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002200_01	euxassay_002200_02	euxassay_002200_03	euxassay_002200_04	euxassay_002200_05

euxassay_002200_06	euxassay_002200_07	euxassay_002200_08	euxassay_002200_09	euxassay_002200_10

euxassay_002200_11	euxassay_002200_12	euxassay_002200_13	euxassay_002200_14	euxassay_002200_15

euxassay_002200_16	euxassay_002200_17	euxassay_002200_18	euxassay_002200_19	euxassay_002200_20

euxassay_002200_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon marginal layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 13 14 15 16 17 18 19 20 moderate expression: see section 10 11 12

olfactory cortex marginal layer

strong

homogeneous

strong expression: see section 14 moderate expression: see section 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6059
Entity Detected:	Nxph3, ( MGI:1336188)
Sequence:	sense strand is shown >T6059 CGGAGGGGGCCTGGGACCCCGAAAGGAAAAGAGAGACGGGTGAGAGCAAAGGAGGAAGATGCAACTGACC CGTTGCTGCTTCGTGTTCCTAGTGCAGGGCAGCCTCTATCTGGTCATCTGCGGCCAAGACGATGGCCCGC CTGGCTCAGAGGACCCTGAACACGATGACCATGAGGGCCAGCCTCGGCCTAGGGTGCCTCGCAAGCGAGG TCATATCTCACCTAAGTCCCGCCCCTTGGCCAACTCCACCCTCCTAGGGCTGTTAGCCCCACCCGGGGAG GTTTGGGGTGTCCTTGGGCAGCCCCCCAACCGCCCCAAACAAAGTCCCTTACCCTCGACCAAGGTGAAAA AAATATTTGGATGGGGTGATTTCTACTCCAACATCAAGACAGTGGCCCTGAACCTGCTGGTCACAGGCAA GATTGTGGACCACGGAAACGGGACCTTCAGCGTCCACTTCCGCCACAACGCCACGGGCCAAGGTAACATC TCCATCAGCCTTGTACCCCCCAGCAAAGCTGTGGAGTTTCACCAAGAGCAGCAGATCTTCATCGAAGCCA AGGCATCCAAAATTTTCAACTGCCGGATGGAGTGGGAGAAGGTAGAACGGGGCCGCCGGACCTCGCTCTG TACCCACGACCCAGCCAAGATCTGTTCCCGTGACCACGCTCAGAGCTCGGCCACCTGGAGCTGCTCCCAG CCCTTCAAAGTTGTCTGCGTCTATATTGCCTTCTATAGCACGGACTATCGACTGGTGCAGAAGGTGTGCC CAGATTACAACTACCACAGTGATACCCCCTACTACCCATCTGGGTGACCTAGATGGGCGGCAGAGGCCGG TGCAGGAGGCTGGCTGGCTGGACACTGGGCAGGGAAAGGGATGGACCTCAGGTAGGGAGGGGGTGGAGAG GAAGAGAGGCCTGGTGGGTCAGGATCAAGCGTCAGGTGTTGGGGAAGGGTCCCCAGTGCTGTGCCCAACC TGAGGTTAGAAGATACTTGTTATGGAAGAGAAATATGCCCTGGGCAGCCTTACAGGGCTTTCCCTCCTAT CCAAAGCAGATGCTGGGCCCCGAGGCCCCTGGGCAGGGAAGCCAGGCAACCCCAGATCAAGCCACAGAGA AGACCTCAACCTTTGTCTCTCTCCTTGCTATTCAGAAGGAGGACAGTGGTCAGCAAAGGGGAGTCCAACT GTGTAGACAACTTAGGATGGAGAGCTCAATGGCGTCTCCCGTGGGAGCCGGCAGTGGGCAGCCCCTTCCC CCCCCCCCTCCCCCGCACTGAGGCTGAAATGGCAACCTCATCCCCACCCTTGGGTGTTTGCTGTCATGAC TCTCGGTCACCAGCCAGGCCATTCCTTTCCAGAAATCCCCTCTCCCTCCCTCCAGCACCTTTGTACCGCT GACACCTCTTGCTGATGGCGCGCCACCCTTGTCCTTCAGCTGAGACAGGATGACTATAGGTCCTGTTAGC ATGAGGGGCACAAAGCAGAAGTCCGCCCATGTGCTGTATGCCCATCCTCTGCCCTAACCACCTGCTGAGA GTGCTCCCTCCCTTGACAGCCAGTTCTTCCTGTAAGACTGGGGCATTCTGGGATAGGGATAGAGCCTCCT TTGCTGGGCGGTGGGGCTATCCGCAGAGGAAGGCTCAGGAGGCTATGTGGCCATGCTGGAACTGTGCCCT AGTGTTGTCTGTGGGGTGTGTGTGGGGGGAGGGGTGGGAAGTCTTGTGAGATGCCCTGTCACTTCTCTGT GCAGAGTCTTGTCCTTGGAGCAGGAAATAAAGCATTCCCAGGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3490479 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490479 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002200 same experiment
	EMAGE:32093 same embryo
	EMAGE:30212 same embryo
	EMAGE:29862 same embryo
	EMAGE:32114 same embryo
	EMAGE:31666 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source