Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29880

2410018G20Rik ( MGI:1922845)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29880 EMAGE:29880 EMAGE:29880 EMAGE:29880 EMAGE:29880
euxassay_007852_01 euxassay_007852_02 euxassay_007852_03 euxassay_007852_04 euxassay_007852_05
EMAGE:29880 EMAGE:29880 EMAGE:29880 EMAGE:29880 EMAGE:29880
euxassay_007852_06 euxassay_007852_07 euxassay_007852_08 euxassay_007852_09 euxassay_007852_10
EMAGE:29880 EMAGE:29880 EMAGE:29880 EMAGE:29880 EMAGE:29880
euxassay_007852_11 euxassay_007852_12 euxassay_007852_13 euxassay_007852_14 euxassay_007852_15
EMAGE:29880 EMAGE:29880 EMAGE:29880 EMAGE:29880 EMAGE:29880
euxassay_007852_16 euxassay_007852_17 euxassay_007852_18 euxassay_007852_19 euxassay_007852_20
EMAGE:29880
euxassay_007852_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2626
Entity Detected:2410018G20Rik, ( MGI:1922845)
Sequence:sense strand is shown

>T2626
GAAGTCCATGTGNGCTGGGCAGCGGCTGGCAAGCGATCCCCAGGACTCGGTGCCCATATCTCTGTCTACA
TCCACATCAGAGACCCGAGGAAGAAACCGAGGTGGCCCCATTCTTGGCAATGTGACCATATCGGCAGAAC
GTGGAAGCCGTGAAAGACCCATCCAGAGAATGCCACGCCAGCCCAGTGCGACTCGGCTGCCCAAGGTGGG
AGGATCTGGAAAAAGCAACTCCCGAAGCAGCCCATAGGCTGGGACAGAGGCTGCACTTGACACCGTCTTC
AAGACAGATGCTATCCACCTTCACTCCTCAAACCCTTCTGAGGAGCTGAATGTCTGTAGCTTTTTGATAC
TATTAAAAAATTTAGATTTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:1400759 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1400759 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007852 same experiment
 EMAGE:29907 same embryo
 EMAGE:29486 same embryo
 EMAGE:31338 same embryo
 EMAGE:32123 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS