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EMAGE:29907

Defb1 ( MGI:1096878)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1328
Entity Detected:Defb1, ( MGI:1096878)
Sequence:sense strand is shown

>T1328
CTCGAGCCTGTTGGCCTACTGGAAAAAACAAGCTCATTCAAGCCTCATCTGTCAGCCCAACTACCTGGGA
GTTTCACATCCTCTCTGCACTCTGGACCCTGGCTGCCACCACTATGAAAACTCATTACTTTCTCCTGGTG
ATGATATGTTTTCTTTTCTCCCAGATGGAGCCAGGTGTTGGCATTCTCACAAGTCTTGGACGAAGAACAG
ATCAATACAAATGCCTTCAACATGGAGGATTCTGTCTCCGCTCCAGCTGCCCATCTAATACCAAACTACA
GGGAACCTGTAAACCAGATAAGCCCAACTGTTGTAAGAGCTGACAGTAGTTTGAAGAATGAGCATAAAGG
ACGAGCGATGGATTGTAAAATTAGTGTTTTAATAAATGAAATGTTTTTGAAGTTTATTTACATCATATCA
AAATAAATTTTATTTCTCTGTTTAGAAGAGCAATTTTTTTTTAAAAAGTATTGGGCTTAGAACAAGAGGT
GAGAAAATCCAGAACATCTGCCTGGTCCTGAGTAATGATGGTCACAATTTGAGTGATATCACTTCTATGT
AATTTTATCACC
Notes:The probe template was PCR amplified from IMAGE:2285954 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2285954 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007846 same experiment
 EMAGE:29880 same embryo
 EMAGE:29486 same embryo
 EMAGE:31338 same embryo
 EMAGE:32123 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS