EMAGE mouse gene expression spatial database

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EMAGE:31338

Mscp ( MGI:1914962)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007768_01	euxassay_007768_02	euxassay_007768_03	euxassay_007768_04	euxassay_007768_05

euxassay_007768_06	euxassay_007768_07	euxassay_007768_08	euxassay_007768_09	euxassay_007768_10

euxassay_007768_11	euxassay_007768_12	euxassay_007768_13	euxassay_007768_14	euxassay_007768_15

euxassay_007768_16	euxassay_007768_17	euxassay_007768_18	euxassay_007768_19	euxassay_007768_20

euxassay_007768_21	euxassay_007768_22	euxassay_007768_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 23 moderate expression: see section 20 21 22

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2637
Entity Detected:	Mscp, ( MGI:1914962)
Sequence:	sense strand is shown >T2637 TGGCCTCGAGCCAGATTCGGCACGAGGATTTTTGACGTTGCAAATTTCAAGATAGGAAAAGGCTCAGTGA CCAGTTTAGGATAGCCATAACCTATCTCGGTCATTGGCATCTTTGGAGTGACATCCAAGGATGGACCATC CCACCTGTGTACAATGATAATACTTTAGCTGTGTGTGTGTGTGTGTGTGTGTGTGTTCAGGCCATGTGAC AGTCAACCATTTAATAGATGAGTGTTAATTTATTATAGATAAATATTGATCTTTAAAAATGGGATGCTCT TAATTCATGACTTTTTAAAGAATTGGATTAAATATAGCTACTTTTATGTGAGCTTTCCCCTCTGTCCCCC TGCCCCTTCTCTCTGTCTCATTTCTCACAACCATCACCCTCCAGTGTCACCCCCAAGGCTATATCTCCAA CATACTGCTATGCCATTAAGGTTTTATTTAACTTTATTTATGGATAATTATGTCTATGAACAACAAATGT GCCGTTACCGGTCACACAGTGTGTGGGATAAGAGTGGCCTAGTGTTGCTTTCCGAATGTCTTAAAATACC TG
Notes:	The probe template was PCR amplified from IMAGE:1400944 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1400944 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007768 same experiment
	EMAGE:29907 same embryo
	EMAGE:29880 same embryo
	EMAGE:29486 same embryo
	EMAGE:32123 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source