EMAGE mouse gene expression spatial database

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EMAGE:30164

BC021891 ( MGI:2385307)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007143_01	euxassay_007143_02	euxassay_007143_03	euxassay_007143_04	euxassay_007143_05

euxassay_007143_06	euxassay_007143_07	euxassay_007143_08	euxassay_007143_09	euxassay_007143_10

euxassay_007143_11	euxassay_007143_12	euxassay_007143_13	euxassay_007143_14	euxassay_007143_15

euxassay_007143_16	euxassay_007143_17	euxassay_007143_18	euxassay_007143_19	euxassay_007143_20

euxassay_007143_21	euxassay_007143_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5870
Entity Detected:	BC021891, ( MGI:2385307)
Sequence:	sense strand is shown >T5870 CCGCCACCGGTTGTCCGCGCTAGCGGCTGCGGGGAAGAACTAGCCAAACTGGAGGCAGAGCAGAGGTCAG AGCCCCCGGGCAGTGGTCGTAACCTCTGTAGTCCTGCTCCAGCCCTGCACCCTTCCCTGTTAGCTCGGAC CGATGATACGCGTCGCTGGAGACCCGGAAAGCTGAGGTTGTGGCTGTCGCACTTGGTCCACTAGCGACCT CTCAATCGCCCCTATGGCTCTACCTGTAGCCGAGGGCACGGCTGACACGCCGCTGTCCCCGGCCCGGGAC GACTCGGGGAGTACCTCCTCCGGGATGTGGGCCGCGCTTTATGATTACGAAGCGCGCGGTGAGGACGAGC TGAGCCTGAGGCGCGGTCAGCTGGTCGAGGTACTGTCGCAGGACGCCGCGGTGTCAGGAGACGAGGGCTG GTGGGCCGGCCAAGTGCAGCGGCGCCTGGGCATCTTTCCGGCCAGCTACGTGGCGCCCTGTGGCCCGGTG CCGCCGCCGGCGCCGCCCCCGCCGAGACCCTGCTCGCCTGTGCACGTAGACTTCGAGCGCCTGGAGCTCA AGGAGCTGATCGGCGCCGGCGGCTTCGGTCAGGTGTACCGCGCCACCTGGCAGGGCCAGGAGGTGGCGGT GAAGGCGGCGCGCCGGGACCCGGAGCAGGACGCGGCGGCGGCGGCCGAGAGCGTACGGCGCGAGGCGCGG CTCTTCGCTATGCTGCGGCACCCCAACATCATCCAGCTGCGGGGTGTGTGCCTGCGGCAGCCGCACCTCT GCCTGGTGCTGGAGTTCGCGCGTGGCGGCGCGCTCAACCGCGCGCTGGCGGCGGCAGCATCGGATCCGCG AGCGCCCGGCCCCCGCCGCGCGCGCCGCATCCCCCCACAGGTTCTGGTCAACTGGGCGGTGCAGATCGCC CGCGGCATGCTGTACCTGCACGAGGAGGCGGTGGTGCCCATCCTGCACCGTGACCTCAAGTCCAGCAACA TTTTGCTGCTGGAGAAGATAGAACATGATGACATTTGCAACAAGACCCTGAAGATCACGGACTTTGGGCT AGCGAGGGAATGGCACAGGACCACCAGGATGAGCGCAGCAGGGACCTACGCCTGGATGGCTCCCGAGGTC ATCAGGTCCTCCCTGTTCTCCAAGGGCAGCGACATCTGGAGCTATGGAGTGCTGTTGTGGGAGCTGCTCA CTGGTGAGGTCCCGTACCGTGGCATCGATGGACTGGCTGTGGCGTACGGGGTGGCTGTCAACAAGCTCAC ACTGCCTATTCCATCCACCTGCCCTGAACCGTTTGCAAAACTCATGAAAGAGTGCTGGGAACAAGACCCT CATATCCGCCCCTCGTTTGCCTTAATTCTTCAGCAGCTGACTGCGATCGAAGAGGCAGTGTTGACCAACA TGCCCCAGGAGTCCTTCCATTCCATGCAGGAAGACTGGAAACTGGAGATTCAAATGTTCAGTGAGCTGCG AACCAAGGAGAAGGAGCTGCGTTCCCGAGAGGAGGAGCTGAGCCGAGCTGCCTTGCAACAGAAATCGCAG GAACTGCTGCTCAGACGCCGCGAGCAGCAGCTCGCGGAGCGTGAGATCGACGTGCTGGAGCGCGAGCTGA ACGTGCTCATCTTCCAGCTGAGCCAGGAGGCTCCCCACGTGAAGAAGAGGAAGGGAAGGTTCAGGAGAGG ACGGCTGAGGCTCAAAGACGGCCACCGCATCAGCCTGCCTTCAGATTTCCAGCACAAGATCACAGTGCAG GCTTCCCCGACGCTGGACAAGCGCCGAAGCTCAGACAGCGGACTGTGCAGCCCACCCGGCAGCCCCTTGA TGCTCCCCCGCCTCCGGGCTATTCAGTTAACTTCAGATGAGAACAATAAAACCCGGGGAAGGAACATGGT CTTCCGCCAAGAAGACTTTGAAGACGTGAAAAGGAGTTTCAAGAAAAAAGGATGTACGTGGGGACCAAGC TCCGTTCAAACAAAGGAAAGACCAGAAGGCAGAGAGAGGGTCAGACCTCTCTCAGATGGCAACAGCCCTT GGTCATCTCTTTTAATAAAAAGCCAGAAAACCACACCCTTGGCTTCACTGTTTGTGGACCAGCCAGGTTC TTGTGAGGAGCAGAAACTTGTCCCTGAAGGATTAGAACATAGAAAACCAAAGCAAACGAAGTTCCCTGGC CAGGCCCACGTTGGTCTACCTCTTTGCAAAGACAGCCAGAGAGAAGACTCATCAGAGGCTGAGAGTCGGG AGGAGGGATCCCCTAAGGGGTCCCCGGTGAACAACGTGGGTGCACCCATGCTGAGGAAGAAAACGGAGTC AGCGCTGTGTGAGTGTGGGATGCTGCTGGCATCGATGGCTTTGGGACTGGATGTCAGGAAACTTCACGGA GCCCAGGCCCCCGCAAAACCATCACCCAAGATGGAAAAGAAGGAAGAGGGTGCCCTCCAACCTGCCTCCA GGTGCCAATCAAGTCCCAGTTCCCTTCTGAGGCAGCCATCTGCAGGCAGGGCACCCAGCGGTGGCTCCAC GCTCCTGCTGCCCAGTGCCCCAAGCCATTCCTCAAAGTCTTCTCTCTCCATGAAGTGTTTGCTGCAGGCT GGTAAGGAGGAGTCATCTTTGGGGAATGCTCGGGACTTGTGTGGTCCTACGACGCTCACTCCAGATCCTG GCTCTGCCGCACCAGAGAGCGGCTGTGAACTCATCCCCGGACTGAGACCTAAGACTGACTATGGTGTGTT GAGAAGCATGCCGCATGCCATCCTGGAGCAGACAGGGGAGAGACTGCCAGGCTGTGCCATTGTAGGAGAC AAAGGATGCCACCACATGCAGATGGGATCTGAAGAAACTCCTCTCTGGCTCCAAAGTGCCCCTGAAGACT CTGGATTACCACACTCTCCCTCCCCTGGACCTCAAAGGGACCTGGCCAGCCAGGCCTCTCTTGTGAAACC TGAAGGTGTTCTGGGCGAGTGCCAGGCATGCCCGGCTCTGCCACAAAGGCCACACACTGCTTCGGTGAGA ACAACATCTCCACCTACCTGGGTTTGTGACAAGGACCATCAGGTGCCAGCCCTTGCCTGCTTGCTTGGTG CTCAGGAGAGAAGCAGGTGTCAGACTCCTTCCTTGCTGGATGCCAGCATAGAAGGTCAGAAGAAGGACTG TGCCATGCCCTTGTGTAGAGTGAAGAGCGTGATGTGTCAGCCGTCCATCTACGCCCTGGAGAAAGACTTT CTGACCTGATGACTTCTGCCTTCCCGCCATGATCATTTGATGGGGGCACACGGAGGCACCATGTGTCTTG TGCTGATTCTGAGGAGCACTAGTGACTGCTATTAGCTGCTGTATCACGGCCACTTCATGCTAGTTACCCA GTGACTTGAAGTACACAGAAGTTCAAATGAGTGTCAGCGGAGGTGGGGCCTTCCTAGCCAGTTCCTGTGC TTCAGTCTTACGTATGTCCTGCCCATGAGGACACAATTGGCGAAACTGCAATGGCCGATTCTCGTAGCTT CCTGCCTTCATCCTTTTCTCTCTTTTAAAAAGTCCTTTTGGGGTTTTATTTGTTTGTTTTTTTCCACCAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3156324 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3156324 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007143 same experiment
	EMAGE:30833 same embryo
	EMAGE:30001 same embryo
	EMAGE:29951 same embryo
	EMAGE:31080 same embryo
	EMAGE:31149 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source