EMAGE mouse gene expression spatial database

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EMAGE:30189

Smurf1 SMAD specific E3 ubiquitin protein ligase 1 ( MGI:1923038)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003566_01	euxassay_003566_02	euxassay_003566_03	euxassay_003566_04	euxassay_003566_05

euxassay_003566_06	euxassay_003566_07	euxassay_003566_08	euxassay_003566_09	euxassay_003566_10

euxassay_003566_11	euxassay_003566_12	euxassay_003566_13	euxassay_003566_14	euxassay_003566_15

euxassay_003566_16	euxassay_003566_17	euxassay_003566_18	euxassay_003566_19	euxassay_003566_20

euxassay_003566_21	euxassay_003566_22	euxassay_003566_23	euxassay_003566_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6707
Entity Detected:	Smurf1, SMAD specific E3 ubiquitin protein ligase 1 ( MGI:1923038)
Sequence:	sense strand is shown >T6707 CGGACGCGTGGGCTCGGGAGGCGGCGGCGGAAGCAGCGAGGGCGGCGGGCGTCCGGCTGCGAGCGCGGCG GCTCCGGGCTGGGCCAGCCAGGGGCGGCTCCTGGCGGCGGCGCGGCCGAGCGCTCGGTTCTACGGTTCCA GCCCGTGGTCTCCGGCCCAACGCCTGCGCCGCGCACTCGGCGTCCCGGCTCGGACCCCGGCGCCCAGCCC GTGGCCGTAACCTTGAGGCGGCGGCGGGGCCGGGCCGGGCCGGGCTGGGGGGCGGCGGCACTGGATCCGA GGCCGGCCGGCCGCTGGCGGGAGATGTCGAACCCCGGGACCCGTAGGAACGGCTCCAGCATCAAGATCCG TCTGACAGTATTGTGTGCCAAGAACCTTGCAAAGAAAGACTTCTTCAGACTCCCCGACCCCTTTGCCAAG ATTGTTGTGGACGGCTCTGGGCAGTGCCACTCAACCGACACTGTGAAAAACACCCTGGACCCAAAGTGGA GCCAGCACTATGACCTGTATGTTGGGAAAACGGACTCGATAACCATCAGTGTGTGGAACCACAAGAAGAT CCACAAGAAGCAGGGGGCTGGCTTCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTGAAA GACACTGGCTACCAGCGTTTGGATCTATGCAAACTAAATCCCTCAGATACTGATGCAGTTCGTGGCCAAA TAGTGGTCAGTTTACAGACCCGAGACAGAATAGGCGGTGGAGGGTCAGTGGTGGACTGCAGAGGGCTGCT GGAGAACGAAGGAACAGTGTATGAAGACTCAGGCCCTGGAAGGCCGCTCAGCTGCCTCATGGAGGAACCT GCCCCGTATACAGATGGTACTGGTGCAGCAGCAGGAGGCGGGAACTGCAGGTTTGTGGAGTCTCCAAGCC AAGATCAGAGACTCCTGGTACAGCGACTCCGAAATCCTGAGGTTCGAGGGCCCTTACAGACACCCCAGAA CCGACCACATGGCCACCAGTCGCCAGAGCTGCCTGAAGGCTATGAGCAAAGGACAACAGTGCAGGGACAA GTTTACTTTTTGCACACGCAGACTGGAGTCAGCACATGGCATGACCCCAGGATCCCCAGAGACCTTAACA GTGTGAACTGCGATGAACTTGGGCCACTGCCTCCAGGCTGGGAAGTCCGAAGCACAGTGTCGGGAAGAAT CTATTTTGTAGATCACAATAATAGGACAACCCAGTTTACAGATCCACGGCTTCACCACATCATGAATCAC CAGTGCCAACTCAAGGAGCCCAGCCAGCCGCTGCAGCTGCCCAGTGAGGGCTCCGTGGAGGACGAGGAGC TTCCTGCCCAGAGATATGAGAGGGACTTAGTCCAGAAGCTCAAAGTTCTCAGGCACGAGCTCTCTCTTCA GCAGCCCCAGGCTGGTCACTGTCGAATAGAAGTCTCCAGAGAAGAGATATTTGAGGAGTCGTATCGCCAG ATCATGAAGATGCGGCCAAAAGACCTAAAGAAGCGCCTCATGGTGAAGTTCCGAGGGGAGGAAGGTTTGG ACTATGGTGGAGTGGCTCGGGAGTGGCTGTATTTGTTGTGCCATGAAATGTTGAACCCGTACTATGGACT CTTCCAGTATTCCACGGACAATATTTACACACTGCAGATCAACCCAGATTCTTCTATCAACCCTGACCAT CTGTCTTACTTCCACTTTGTGGGTCGCATCATGGGTCTGGCTGTGTTCCACGGACACTACATAAATGGGG GTTTCACAGTTCCGTTCTACAAGCAGCTCCTGGGGAAGCCAATCCAGCTGTCGGACCTGGAGTCCGTGGA CCCAGAACTGCACAAGAGCTTGGTGTGGATTCTAGAGAATGACATCACGCCTGTGTTGGATCATACCTTC TGCGTGGAGCACAACGCTTTCGGGCGGATTCTCCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCTG TCACTGAGGAGAACAAGAAGGAATACGTCCGGCTGTATGTGAACTGGAGGTTTATGAGAGGAATCGAAGC CCAGTTCTTAGCACTTCAGAAGGGGTTTAACGAACTCATTCCCCAACACTTGCTGAAGCCCTTTGACCAG AAGGAACTAGAGCTGATAATAGGTGGGCTGGATAAGATAGACCTGAACGACTGGAAGTCCAACACCCGGC TGAAACACTGTGTGGCAGACAGCAACATCGTCAGGTGGTTCTGGCAGGCGGTGGAGACCTTCGATGAGGA GAGGAGAGCCAGACTCCTGCAGTTTGTGACAGGATCCACAAGAGTTCCTCTCCAAGGCTTCAAGGCTCTG CAAGGCGCGGCAGGGCCCCGGCTGTTCACCATTCACCTGATAGACGCCAATACAGACAACCTGCCCAAGG CCCATACCTGCTTTAATCGGATCGACATCCCACCCTATGAGTCCTATGAGAAGCTCTATGAGAAGCTGCT GACAGCGGTGGAGGAAACCTGTGGCTTTGCAGTGGAGTGAAGAGCAGCCAGTGGCCACAGAGTCTTGCTC ACAACCACCAGACCCAAAGCGTATGGCGTCTGCACGCCTCCCGCGTGGCGGGCTGAGGCCTGAGGTTCCA GAAACCAAGGGAAAAGGCTCGTCTCCCTCCTCCTTTGGAGAGGGCAGGCCAGGGGACTTTCCTAGGTGGC TCCCACCCATTTATTCTCCTTTATTATAGTTTGCCCACCCCTCCATCACCCATCCAATAAAACGCAGCCA GGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3660965 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3660965 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003566 same experiment
	EMAGE:31182 same embryo
	EMAGE:30377 same embryo
	EMAGE:30376 same embryo
	EMAGE:30203 same embryo
	EMAGE:30378 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source