EMAGE mouse gene expression spatial database

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EMAGE:31182

Hsbp1 heat shock factor binding protein 1 ( MGI:1915446)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003563_01	euxassay_003563_02	euxassay_003563_03	euxassay_003563_04	euxassay_003563_05

euxassay_003563_06	euxassay_003563_07	euxassay_003563_08	euxassay_003563_09	euxassay_003563_10

euxassay_003563_11	euxassay_003563_12	euxassay_003563_13	euxassay_003563_14	euxassay_003563_15

euxassay_003563_16	euxassay_003563_17	euxassay_003563_18	euxassay_003563_19	euxassay_003563_20

euxassay_003563_21	euxassay_003563_22	euxassay_003563_23	euxassay_003563_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 17 18 19 moderate expression: see section 08 09 10 11 12 13

facial vii ganglion

moderate

regional

moderate expression: see section 04 06 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 09

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

cervical ganglion

moderate

regional

moderate expression: see section 10 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 12 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

thoracic ganglion

moderate

regional

moderate expression: see section 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5974
Entity Detected:	Hsbp1, heat shock factor binding protein 1 ( MGI:1915446)
Sequence:	sense strand is shown >T5974 GGTTCCGAGACATCGCCGCCAAGCTGGGCACCGGGGAGATGGCCGAGACGGACCCCAAGACCATGCAGGA CATCACCTTGGTGGTGGAGACGCTCTTGCAGCAGATGCAAGACAAGTTTCAGATCATGTCAGACCAGATC ATCGGAAGGATAGATGACATGAGCAGTCGGATTGACGACCTGGAGAAGAATATCGCTGACCTCATGACCC AGGCTGGAGTAGAAGAACTGGACCCTGAAAACAAGATTCCTACTGCCCAGAAGAGTTGAAGGCTGCCGGT ACTGAATCCAGAATGTTGTTGTCCCGAGCCAAGACAAAAACCAAGTGGCTTTCTGCAACTAACTCCTATG TGTGGACAGGTTTATAAACGTGCATTCTTATCACAGCCTGCATAGCCTAGAGTACCCCTACACCCCATCC CTCAAACTTGCTATCTTCACATCCTGGTCAGTTCTATTAATTATTAAACACTAAAATTTTGGCGGTTTCT GGATAAGATTGTCAAACGTTTTAGTGATTTTTTTCTGAGTTCCCCTCCCCACTGCTCCTCTGTGGTAGTT GCTGTGTGATAAAGTGGCAGAGTACTCGTGATCACGTGACCACAGCCCCTCCCCTGGCAGAAGCCGTTCG TGAGCCGTGAGTGTGTAGGGTTCTTTGATTTTCAGTGCAAAGTGACTGTTGAGCCGCCCGCCACTGGAGT GTCAGAAGTACTTCTCCAGATGGCCCAGGACATTTAATAGTTGTTTGTGCCGAAGCGTCTCTCAGAGTTT TGCATGAGATTCTAGTAGCGTTGGTAGGACCTGCCATGCTCATCCGGCAGGGTATCAGAGCCCAGTGACC CTGTCCTCCCTTCAAGCTACTGACTGAAAGGGAAGAGACCAAAAGACCCAAGTGAGCCCAGCCCTGTCAG CAAGGCTGAAAAATGCCCTTTGACTTGTGGGAGGTGCTGGTTTTTAAGAAATACTATTTTACTTGAACTA TTTCTTGTCTAAATTCTTGCACAATGAGTTCTATTATTGATTAAAGAAGACAGATTTACATTCAGGATTC TGGTGTCTGGAGGCTTAGCCTCAAAGCCAGTTTTGATGACTTAGAAGGCAATCTATGTCACTGTCCCCAC AAAGTGATTTCATACAATAAAAACCAAGAGTAATTAAACATTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3485301 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3485301 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003563 same experiment
	EMAGE:30377 same embryo
	EMAGE:30189 same embryo
	EMAGE:30376 same embryo
	EMAGE:30203 same embryo
	EMAGE:30378 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source