EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30382

AI597479 ( MGI:2138299)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006619_01	euxassay_006619_02	euxassay_006619_03	euxassay_006619_04	euxassay_006619_05

euxassay_006619_06	euxassay_006619_07	euxassay_006619_08	euxassay_006619_09	euxassay_006619_10

euxassay_006619_11	euxassay_006619_12	euxassay_006619_13	euxassay_006619_14	euxassay_006619_15

euxassay_006619_16	euxassay_006619_17	euxassay_006619_18	euxassay_006619_19	euxassay_006619_20

euxassay_006619_21	euxassay_006619_22	euxassay_006619_23	euxassay_006619_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6627
Entity Detected:	AI597479, ( MGI:2138299)
Sequence:	sense strand is shown >T6627 CTTCCGCCGCAGTGCCTTGTGGGAGGCCGGCCAGGGGGTCTCCTGGCGACAACCATGGCGGGGGATGTGG GCGGTCGCAGCTGTACGGACGCGGAGCTGCTGCTGCACCCTGAGCTGCTATCCCAGGAGTTCCTCCTCCT CACCCTGGAGCAGAAGAACATAGCAGTTGAAAATGAAGTAAGGGTAAACAAAGACAACCTTACCGACCTT TACGTCCAGCATGCCATACCGTTGCCTCAGAGGGATTTGCCAAAGAATAGATGGGGGAAGATGATGGAGA AGAAAAGAGAGCATCATGAAGTGAAAAATGACACCAAAAGGAGTAGTGCTGTAGATGGATTAAGGAAAAG GCCCCTCATTGTGTTCGATGGGAGCTCAACCAGCACAAGCATCAAAGTGAAAAGGACGGAGAACGGAGCA GACGACCGGCTCAAGCCTCTTGCCCAGATAGGCTCCACCAGTGATGCCTTCTGGAAATCACCAAATTCCT CGTCGAGGATTTCACCCCTAGTGTTGTTTTCCAATTTGCCTGTGAACCATAAAATGGAGCACAATAATAA TGACACTCAACAGAACCATGACTTAATGAATAGGAAAAGTCCTTCGGGTCCAGTGAAGTCACCACCTCTG TCCCCTGTTGGAACTACTCCTGTGAAATTAAAGCGGGCTGCTCCTAAGGAAGAGGCAGAGGCCACAAATC ACCTGAAGCCTCCAGAGGTGAAGAGGAAGATACAGCATGTTACATGGCCGTGAAAGCAAGCCTCCAAAGT ATAAACAGAGCTGCAGCCGCAGTTTTCAGATGAGTCCTGTACAGTATCTATGGAGGTCCTGAATACTGTG GGACTTTCTTGAGGTTTAAAATAGGTTTGGAAAAACAAGTCTTTCCCTTCTCTCCTTCCCTCTATTCCTT CTTCCCTTCCTTACAATGTCTTGACTTTCATGTCCCAATTAGGGAAGAATTTCTTTTTAAAATAGAGCTT TGTACACATCCTCTAAAAATTCTTTTCCTAAGCAGAAGCATAAAGCAGTTCACAGTATTGTGGTAAATAT TGCCACCTGCATCTTGAAGGGTTCGCCAAGAAAAGTGTGTTGATGGTGTTGATGGAAAAGCTTTCTGCTC TTTAGTTACGTGTGCCATTTTGTCCCATGGAAAGCAAAAGGGAAGTCAGGGCTTGCCATTCCTCCTTCTC AGTGGCGTAAGCTGCTGTGTCTTTAGAATGTTCCTGGCTTACAGGGACCCTGCCTACTCTTTTCTTATGT GGCTTGTATGAGCTTTATCTTTTTAAAATGAGACTGATTTCAAAGTCTTCAGGCTGGGTGATCATGTTAA AGGCAGTACTCTGTGTCGGAAAGTTTGGGAATGATGGTCCTAGAATTCTAGACATAAGAAAATCCAGAAC GTGTGACAGTCGTGGTGGGGTGCTTATCAGTATGCTGTGGAGTAGGCAGTTGTCATTATCAGGACGCTGG AGTTGGAATTCACTGTAGAATGCAGTAGGAAGTGGACTCTGTTGAAGAGGTTCATGTGCTTCCTGCAGGG CTTCCTGCAGGGCTGTCCCATGCAGGGCTAAGTGATGCTCTTTTTATTTTTGTGCAAGTAGCATTATCCA CTTGTATAATGCAAGAATGTGAGAAAATACTGTTTGCTTTACATTCGTGACGTCTAGCGTCTGCTCCTTC CTTTTGTTTCTTACTAAGAAACATTTTAAGCAAAAGATACTTCATAGATTTTTCCATATATAATCTTCAC ATTGTTGGTTTGCCTCGTTAGTATGCCTTAGTGTCATCTCCTTTCTTTGGTTTGTTAAAACCACCATTTT AGCTCACTTATCAGAACAGCCTTACAGAGATAATTCACCCTTGCAGGCCAGCGCTGTGCTTAGACACCAA CCCTGTAGGTTTCTGCTTTTTTTTTCCAGACTAAATTTGTACACATAAGACAAAATTTATGATATTAAAT ATTGCATGAATTGTTGATGCTACAGTAAGGTTTTAAATACATTGTTACCTACGGGGTTGATAAGTTAATA TTGTTGAACTGTGCTCCTATATGTAATCGTCAAGGTACATTTTAAAAGTGCAGCTTTTAGAGTACAACAA CACATTGTATTTTATATTCAGTTGACCTCTCTGCTTCCACTGGCGTTTCTCTTTTTAAGGAGAGAGTTAG CCATTTGACGGTTTTAAGTCAGTGGGAACTTATTTTTAGTTACTTGGTGAAATTAAATTTTAATTTGTAT ATTTAACGTACAGAGTAGGTTGGTAATAACAGCTGAACTGTGTAACATTGTTGCTTCAAATTGAAGTTTA TACTATGAACACTCAGCATCTACGCTCACATAGCAGCATATTGTTGAGCTGTCTTGAGTTTGAGAAGTGA AGACGCATTGACCCATGCACTGTGTATTACGTAGTGCCTCCGTCACGGAGCGCCATGCCTCCTGAAGGGA TCATGGAATTGGCTTCCTCCATGCGATGAAGCATCGTCTGCTGGGCATCTGGAGCAGCAGCATTGGGAGG GTTGTTCTGAAGTAGTGTTTCAGTTTGGGCTAGAAGGACTTGATGAGAGTGGTGAACAAGTCTGGAGTAA GTTTCCTTAATAGTGTATAGGGTTAGTATTGTTGCTTTTGATTCTGTATTCCAGTGAGGCAAGATATTCT TATTCCTGGGTTGGGCAAATAGATGGCATTAGAGGCAGTGGGAAGCTCTTGAGTTACAAAAGGCACCACA TCCACCATCTGCAAGTCAGCCTCCTTTTTTTCCTACTTTGTATCTCCTGGTCTCTTTCCCGTTCTCCTTC CTCTTAGGTCTAAGCTTCAGACTTAGGTTTGCAGATTGTATTGTGTCTGTGACCTTACTCCATTCACTAA GGGGTGAAGATAGTCTGACCCCAATCCTCTTCATCTGAGGGGGTGTTAGCTCACTGTGTGGATCCTACAG TCTGGAATCCTGCAGCACCAGGGCACTGAATTGCTTCCCAAAGGCTGTCACAGCAGCATAGCTTTCCAGG GTAGCCTTATTAGTGTAGGCAGCTGCCAGACCCTTGGAAGAGTGGCTCACTACTGCCATAAGGCTGTATT TGCTGGTATTAAAATTCTTCAGAATTGAAATTTTCATTTGAAATTCTGTTTTGGTTGACTTAAGTTTGAG AGACAGTTCAAAAACTGATGTAGAGACTCATTCAACAGCAATGACTTTTTAAAAACTTACATTAAGTAAA ACTGCCAGTAGATTAAATCCTCCCCATACGTAAGAGCTTTCTCTACTCACTACTGTGGAATCCGAGCATT GTTGAGAGGCTACTAATCGGTCAACATGTTTTTAAGTGTGCTTTTATTAAATGCTTGTGTACGTTTTGTA ATTCAGTGTAGAAAAGGTTGACCTTGTAATGTACATTTTTTGTATGTATAGCCTTTTAAGTAGCCTTGTC CTTATTTAAATAAACTGAATAAAACTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3598975 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3598975 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006619 same experiment
	EMAGE:30384 same embryo
	EMAGE:31593 same embryo
	EMAGE:30035 same embryo
	EMAGE:31327 same embryo
	EMAGE:30941 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source