EMAGE mouse gene expression spatial database

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EMAGE:31327

Ifit2 interferon-induced protein with tetratricopeptide repeats 2 ( MGI:99449)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006667_01	euxassay_006667_02	euxassay_006667_03	euxassay_006667_04	euxassay_006667_05

euxassay_006667_06	euxassay_006667_07	euxassay_006667_08	euxassay_006667_09	euxassay_006667_10

euxassay_006667_11	euxassay_006667_12	euxassay_006667_13	euxassay_006667_14	euxassay_006667_15

euxassay_006667_16	euxassay_006667_17	euxassay_006667_18	euxassay_006667_19	euxassay_006667_20

euxassay_006667_21	euxassay_006667_22	euxassay_006667_23	euxassay_006667_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

strong

regional

strong expression: see section 07 08 09 12 13 14

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 09 10 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7919
Entity Detected:	Ifit2, interferon-induced protein with tetratricopeptide repeats 2 ( MGI:99449)
Sequence:	sense strand is shown >T7919 CCGAAAAGCAAGTTCTGGCCTTCTGCAGTTAATGCTTTTTTACCCATCAGCAAGATGCACCAAGATGAGA CTTAGAGGTGCTGCACAGAAGCAGGAAAAGGAACCAGCTGCTCCTATATATAGGTCTGGAGCACTTCATT AGAAGGCAGAGGAAGAGGTTGCCTGGAGAGTGGCTGCAGCCCTGCTTTGAGCGCTTTGACACAGCAGACA GTTACACAGCAGTCATGAGTACAACGAGTAAGGAGTCACTGGAGAGCAATCTGCGACAGCTAAAATGCCA TTTCACCTGGAACCTGATAGCAGAAGATGAGTCCTTGGATGAGTTTGAGGACAGGGTGTTTAACAAGGAT GAGTTTCAGAACAGTGAGTTTAAAGCCACCATGTGCAACATACTGGCCTATGTAAAGCACTGCAGAGGTC TAAATGAGGCAGCACTGCAATGCTTAGGGGAAGCTGAGGGCTTCATCCAGCAACAGCATCCTGACCAAGT AGAAATCAGAAGTCTGGTCACCTGGGGAAACTATGCTTGGGTCTACTATCACATGGGCCAGTTCTCAAAG GCTCAGGCTTATCTGGATAAAGTGAAACAGGTCTGCAAGAAGTTTTCCAGCCCCTACAGGATTGAGAATC CTGCTCTTGACTGTGAGGAGGGGTGGGCCCGATTAAAGTGTACCAAGAACCAAAACGAGAGAGTGAAGGT GTGTTTCCAGAAGGCTCTGGAAAAGGACCCGAAGAACCCAGAATTCACCTCTGGATGGGCCATTGCGAAC TACCGTCTGGATGACTGGCCAGCAAGGAATTACTGCATTGACTCTCTGGAGCAAGCCATTCAACTGTCTC CTGACAACACTTATGTTAAAGTCCTCTTGGCACTGAAGCTTGACGCGGTACATAAAAACCAAGCAATGGC GCTGGTTGAAGAAGCCTTAAAGAAAGACCCAAGTGCAATAGATACACTCCTCAGGGCAGCCAGGTTTTAT TGCAAGGTATATGACACAGACAGAGCTATACAGTTGCTTAGAAAGGCTTTGGAAAAGCTGCCAAACAACG CCTATGTGCATTACTATATGGGGTGCTGTTATAGGTCAAAAGTCCATCATATGTTGAACAGAAGGGAAAT GGTATTTAGTGGGGATAGGAAGAAGTTAGAGGAACTAATACAACTGGCAGTGAATCACTTACGGAAAGCA GAGGAAATCAAGGAGATGCTTGAATATTCCTGTAGCTTCCTTGCTGATCTTTATATCATAGCAAAAAAGT ATGACGAAGCCGATTACTACTTCCAGAAAGAATTGAGCAAGGACCTCCCTCCTGGCCCTAAACAGTTACT CCACCTTCGGTATGGCAACTTTCAGTTTTTTCAAATGAAGCGTCAAGACAAGGCTATCTACCATTATATG GAGGGTGTGAAAATAAAGAAGAAAACAATCCCCCAAAAAAAGATGAGAGAGAAACTTCAAAGAATTGCCC TAAGGCGGCTTCATGAAGATGAGTCTGATTCTGAGGCCTTGCATATCTTGGCATTTCTTCAGGAGAATGG AGGAGGTCAGCAAGCTGATAAAGACTCTGAGAGGGGAGTGGACTCTGCAAACCAGGTACCTTCAGCATCT TTGGATGAAGCAGGTGCTGAATACTAGTGATGTGGTACCCGGCCTACTGCTGGAAGGAAAAAGAAAGCCC TCACCAAGCTGCCATTCAAAATACTTAACCACTTCCTGGATCAAGAATGGGCTATCATACTGACTATAGT ATTTGTCAGGAGTTTGGGGAACTCTGCTATCAGATTCTAGCACCTGAGTTTCTGGATCGTGGGATGGTCT GGGAGAGGGACCAGATGAGGTGATAGATACAGGATCTTGGCAAACAGCCTAGAGCTCTTTCAATTTTTTT TGGAAAAATAGTTTCCACAGTAGTAAATTCTTTAATCACACCCACCCCTCAGTGGAGGCTTGTCTTGGGC TTGTTTCAGTATTTGTATAAAGCAGCCAAATTCCTCACATGGGTGAATGTTCCATCCTTGAATTATTTTA GATATTGCACAGCATTTTCTTCATAGATAATAGTGTCCATCAAAAAGCTGAGGAGTCAGAGACTGTGGCT CAGGACTGTAATCCTAGCACTTGGAATCTGAGACAGGAGGATTACAGTGAACTTGAGGTCAGCCTGAGCA TGACCTGGAAGGGATGGTGGGAGGTGAGAATTATATGTATAAATATTAATGATTATGTACATTACCTTGT TAGTTTGGGTGAGTATATGTCTATATAACCACTATCACATATATCTAAAAATACAGAAGGGAGAGGTTTT GCTTTATTGAATAACTTTAAGACTTACCAAATAATGTTCAATATTAATTGAGCTCAAAATATAAATTGAA TAGTGAGTCCCAGGTCTGGAAGGGATGGTGGGAGGTGAGAATTAAATGTGTAAATATTAATGATTATGTA CATTACCTTGTTAGTTTGGGTGAGTATATGTCTATATAACCACTACTATCACATATATATAAAAACACAG AAGGGAGAGGTTTTGCTTTATTGAATAACTTTAAGACTTACCAAATAATGTTCAATATTAATTGAGCTCA AAATAGAAATTGAATAGTGAGTCCCAGGTCTAAAAACAAACAACAAAAGTTGTGGGACCAAGTCATTCCT CCAATTACCAGCCAGTTCTCAATCTATGGTGTCCCACACACATGAGTTCAACTGTTCCATTAGCTCAGGT GGCAGCAGGATTCGAAAAACTGAGGACAGGGTGAGTCAGAAGGGTCACATCTGTGCAAAGCAGTAGACTC AGGCAGCCACACCAGACTTCCAGGAGTCGCATGGGAAGATGTCTCCCTACTCTGCCCTCCTAGAACTATA TAGTTGTAGGAAAAGAAGGGAGATTCTGTCTACCACCATGACAATTCATATAAACATTTATTTCAAGAGT ATGGAAACACCAGTGGGGATGAAGGGATTGCTGATTTATACGCGATGATTATAAACTCATGAGGAAAAAG TTTATAGTCACTGTTAGGAAGATTTCTCTGTCAAATAAATAATTTATTCAGAAATGCTATAAAGAAATGC CAGGAAGACAGCTGCCTAGAAGGATAACTTGGATTGATGCTTGGGTCAAGGGACTTTGAAAATGTCATGG GTACTGGAGTTGAGTTGTTTTATTCCATGTGAAATGTGTCACACCAAGGGATATAAATAATTTTTATAAC TCTGAAAAAAAATACAGAAACACCACAAACTTGATAATTGCCATTTCCTAGGTAGATCTGGAAGGGCCTA AATGTTAAGGTTGGTGTGAATTGACAGAGTCAAGTGGCCCAGTATGAGACGGTCAGCCACACTCTGGGAT AAAAAAATGTTTCTCTACTGGGTTCAGCTTGTCTCTATGTATATATGAACATTTTATTCAGCAATTAAAA CATCCATGATTCATAGATTGTTAAAACTAAACATTCTAACAGCTGTATCAGTGCTTCATAATATTTTAAA AATGAAAATATTCTCTATGCATTAAGTCCATCGATATTCACCATTTGAATTTCTGGAAAAATAATAAACT TTGTTTATAAGTAACACATAATGTAACATTAAGCTATGCTCTGGTTCCCCTGAACTTTACTGTACCTTCG TATTTTTAGACTCAAGTACTGATAATAAAGAGTGCATTAACTGTTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6513417 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6513417 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006667 same experiment
	EMAGE:30384 same embryo
	EMAGE:31593 same embryo
	EMAGE:30382 same embryo
	EMAGE:30035 same embryo
	EMAGE:30941 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source