EMAGE mouse gene expression spatial database

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EMAGE:30384

1810043M15Rik ( MGI:1922928)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006615_01	euxassay_006615_02	euxassay_006615_03	euxassay_006615_04	euxassay_006615_05

euxassay_006615_06	euxassay_006615_07	euxassay_006615_08	euxassay_006615_09	euxassay_006615_10

euxassay_006615_11	euxassay_006615_12	euxassay_006615_13	euxassay_006615_14	euxassay_006615_15

euxassay_006615_16	euxassay_006615_17	euxassay_006615_18	euxassay_006615_19	euxassay_006615_20

euxassay_006615_21	euxassay_006615_22	euxassay_006615_23	euxassay_006615_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7936
Entity Detected:	1810043M15Rik, ( MGI:1922928)
Sequence:	sense strand is shown >T7936 GGGAGCCCTGCGAGGCGGGCGGCCTCCAGCTCTCGTCCCCACGTCCCGGCCCGCGAAGTTCCAGGGCGCC GGGGCATGGAAGAGGGGAAAATGGACGAGAATGAATGGAGTTACCATGGCGAAGGCAACAAGAGCCTTGT GGTGGCCCACGCGCAGCGCTGTGTAGTTCTGCGTTTTCTGAAGTTTCCTCCAAATAAGAAGAAGACCTCA GAAGAGATACTTCAGCATCTGCAGAACATAGTAGACTTTGGCAAAAATGTCATGAAAGACTTTTTGGGAG AGAACTATGTTCATTGTGGGGAAGTTGTTCAGCTGCCTTTAGAGTTTGTGAAACAACTCTGTTTAAAGAT ACAGTGTGAAAGACCAGAGTCCCGTTGTGACAAGGACCTGGACACATTCAGTGGTTATGCTATGTGCCTT CCCAATTTAACCAGACTTCAGACCTTCCACTTTGCAGAGCACCGGCCGATTCTGTGTGTAGAGATTAAGC CAAAATGTGGGTTTATTCCTTTCTCAAATGATGTTACCCACGAGATGAAGCACAAGGTGTGCCGATACTG CATGCACCAGCACCTCAAGGTAGCAACTGGAAAGTGGAAGAAAATCAGTAAATACTGTCCCCTCGACCTC TACTCAGGAAATAAGCAGCGCATGCACTTTGCCCTCAGAAGTCTGCTGCAGGAGACACAGAATAATCTTA GGATATTTAAGAATGGAGAGCTGATTTATGGTTGTGGTGATGCCCGGAGCCCTGTGGCTGACTTGAAGGA GCTTGCACACCACCTGAAGCCCTTCTTCTTCCCTTCCAACGGCTTGGCCAGTGGGCCTCACTGCACGAAG GCTGTGATCCGTGAGCTGGTGCATGTTATCACCAGGGTGCTGCTGAGCAGCTCAGAAAAAGCCCGAGCTG GTGCCCTGAGGCTTGGGCTCCAGGGCCCACGAGTCTGTGAAGCCAGTCCTTTCAGCAGGAGCCTACACAA CCAAGGAAAAAACACCTCAGAGCACTCGGGGTTACCGAAGGGCTGTCTTCTGTACAAAACCCTCCAGGTG CAGATGTTGGACCAGCTGGACATCGAAGGCCTCTACCCTCTGTACAAACGGGTTGAGCAATACCTGGAGG AGTTTCCTGAGGAGAGGAAAACATTACAGATAGATGGGCCTTATGATGAAGTATTTTACCAGAAACTGCT TGATCTGTCCACTGAGGATGATGGGACAGTGGCCTTTGCATTAACAAAGGTGCAGCAATACAGAGTAGCC ATGACTGCCAAGGACTGTTCCATCATGATCGCACTTTCCCCTTGTCTACAGGGCACCAGCTCTGACCAGA GGCCTGTTATTCCTTCGTCAAGATCCCGGCTGGCCTTCTCTGTGTCTGTATTGGACCTCGACCTCAAGCC CTATGAGAGCATTCCTCATCAGTATAAACTAGACAGCAAGATAGTCAACTATTACTCAAAGACTGTGCAT GCCAAAGATGACACTGTGAGGTCGACTCGGTTCAAGGAACATGAAGATTGCACATTAGTTCTCCATAAGG TCTAACTTTTCCCCTGCGGTGTCTTTGAAACTTGAATGTGAAAGTTGAATGGCAGAGCTATTTTCGGTTG TGTTGGGTGACTTGGCGGTGAATGTCTTTGCTTTTAACCCGGGTCTGCTGAGGTTAGACTGCCTCTCCAA GACAAGGAGTTGAAAGCCCTATAGTCTTCTAGGACGAGGAATGTAGGACGTGAGGCTGTGTCCCAGCAAG CCATCCGTTTCCTTAGAGTGCAAATGTCCCTTGAGCCCCAGGCAAACATCCTGGGTGGTTCTTCCAAGCA GAGCTCACGTCCAGTTCTCATACGAAAGCCTTATTATTAGGACCCACGCTTTGGATCTCCTACCACCAGA CCATTGACACAGAAACTTGAATTGTTACGAGTAGCTTACGGTTTGGATTTTCACGAAAATATGGATACTA CTGGAACTTTCCCAGCTGTTAACTGGTCACTTGTTCAATGCAAGTCACATATCAACACAGGCTTTGGGTG GTCCCCTGCCGCAGAGCGGTTAGGGCTTAGTATGGACATTGGTTCTGAGGGGTTTAGACTTAGGTCCCTG TGGAGGTACCGAAGCCCATAGCTTTGTACTGGGGCAGCAGCAGGGAACACAGGACTCAGGTCAGGATGGA CACCACTCACATGAGCATTTTTTTTTTTTTTTTTTAAATTCTTTATGATTTTAACTCTAGGGTTGCTGAG GCGTTTTTTCCTACCTGACATTGCTGCTGTAGAGGCTGGAGGCAGAGCTGTAGTTCAGAGCTGCTAAGGT CTGCTGCGTGGGTGTCCTGACAGCCCAGAGTTTTCTTGGCATTCTGGTGTCTTGGATGGTTGAGGATCAA GGTTTCCCTTCCTCCTAAGCCACATTCATCCCTGGGTTAGCTCTGAGGCATCCTGGGTATTTGGGTCTAG GGCCTTACTGTTTTCATCAGGGCTAGATAGAAGGCTCCTAGCTGGCACTGAGAATTCTACGGGGTCACCA GAAGTGTGCCTGGCTGCTATGAAATCATTGGGAGTTTTTGTCTTCATTTGTTTTTGAAATCCTATGGTAG GCTGTACAGATTGATTATTTATATCATCCTTTTGAGAGACTAATGAAGGCTTATTGTAATATAGTAGTAA AACCATGGAACTGTATGAAAAATACTACATGAAAAATGAAGAAAATATTTTGCTGATTGTAAACTTTTGT GGGAAATTTTGTGATAATTTGAGAATTATACTTGTTTGAATCAAGCAACAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6517021 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6517021 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006615 same experiment
	EMAGE:31593 same embryo
	EMAGE:30382 same embryo
	EMAGE:30035 same embryo
	EMAGE:31327 same embryo
	EMAGE:30941 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source