EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30641

Prl5a1 ( MGI:106332)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004983_01	euxassay_004983_02	euxassay_004983_03	euxassay_004983_04	euxassay_004983_05

euxassay_004983_06	euxassay_004983_07	euxassay_004983_08	euxassay_004983_09	euxassay_004983_10

euxassay_004983_11	euxassay_004983_12	euxassay_004983_13	euxassay_004983_14	euxassay_004983_15

euxassay_004983_16	euxassay_004983_17	euxassay_004983_18	euxassay_004983_19	euxassay_004983_20

euxassay_004983_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8028
Entity Detected:	Prl5a1, ( MGI:106332)
Sequence:	sense strand is shown >T8028 CAGCTTTCCTCAGTCGGTACCTACACTGTGGTGGGATTTTCCAGAGATGCGGCTGTCTAAGATTCAACCA CACCAATCAGGGACACTTCTGCTGCTGCTGCTGTCAAACTTGCTCATGTGGGAGAATGTGGCCTCTGTGC CCAGATGTATCATGGAAGATGGAGGTTGCCAGAAGGTCCTCAACTACATTTTTAACATGACAAGCACCAT ATCTGAGAACTTCAACAACCTTTCTTCAGAAACATTAAATGATTTTGATACAGAGTATGATCCCCACCAG AAATTCCAGAACAGGCCCACCATGACCTGTCACACTTCATCCCGCTCTGTCCCAAACAACAAAAGGAAGG CTGAAAGAATGCGGCCTGTGGTGCTTCTGAATGCGACAATTAGAATGCTGGCTGCCTGGAAAAACCTTCT GCACCATGTAGAAAACAACATGGCTGATCTCGATGGAACTCCTTATGTTATCATATCAAAAGTTAAGTTG ATTGACAGACAAATCAAAAAACTCACTAAAAATCTGCAGAACATTAAGACTATACTCAGCCAGGTTAATC CTGACCTTAAGAAAAATGAAGACTACCCTGCCTGGTCAGGAGAGCCATATGTACAGCAATCAAAAAGGAG AGTTCAACTTTTTGGTTTGCATAGTTTGTTTTTCTGCTTGAACAATGATGCACAGAAGGTTTCTGATTTC ATCAGTATCCTGAGAGACCAAATTGTGCCTAATCAATGAAAAGCTCTCATCCTTCCTATCTATTTCTGGG GCAGTCCATACTGATCCAATCCTCAAAAAGTTCTTTAAGTTTTATAATATTTATGTGTGATTAAATCTAA AGGGTCACTTCTTTTCAAATTTTTATGTTTAATTAAATATAATTACACCACTTCTCCTTCCCTCTACCCA CTGCTTGCTACCCATTGTCCCCTGACTCATTTTACTCTTTATAAAATGAATTTTTATTAATTATAATTCC ACATGCATAATGTATTATTGCTATGTGTATGTATAAATAAATGAATAAATATATAAATGTAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30017843 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30017843 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004983 same experiment
	EMAGE:31550 same embryo
	EMAGE:31602 same embryo
	EMAGE:29954 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source