EMAGE mouse gene expression spatial database

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EMAGE:31550

Wsb1 ( MGI:1926139)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005031_01	euxassay_005031_02	euxassay_005031_03	euxassay_005031_04	euxassay_005031_05

euxassay_005031_06	euxassay_005031_07	euxassay_005031_08	euxassay_005031_09	euxassay_005031_10

euxassay_005031_11	euxassay_005031_12	euxassay_005031_13	euxassay_005031_14	euxassay_005031_15

euxassay_005031_16	euxassay_005031_17	euxassay_005031_18	euxassay_005031_19	euxassay_005031_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 10 11

not examined

regional

not examined expression: see section 01 02 03 19 20

neural retina

strong

regional

strong expression: see section 02 03 19 20 moderate expression: see section 01

vagus x ganglion

strong

regional

strong expression: see section 05 13 moderate expression: see section 12

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 14 15 16 17 18 moderate expression: see section 08

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 13 14

spinal cord

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04 13

trigeminal v nerve

strong

regional

strong expression: see section 14 moderate expression: see section 08

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8065
Entity Detected:	Wsb1, ( MGI:1926139)
Sequence:	sense strand is shown >T8065 AAGAGGTAATCCCGCGTCCGAATCGAGTTCCCGGAATCAGACGGTGCCCCATAGATGGCCAGCTTTCCCC CGAGGGTTAACGAGAAAGAGATCGTGAGATCACGTACTATAGGGGAACTCTTGGCTCCAGCAGCTCCTTT TGACAAGAAATGTGGTGGTGAGAACTGGACGGTTGCTTTTGCTCCTGATGGTTCCTACTTTGCGTGGTCG CAAGGATATCGCATAGTGAAGCTTGTCCCGTGGTCCCAGTGCCGTAAGAACTTTCTTCTGCATGGTTCCA AAAATGTTACCAATTCAAGCTGTCTAAAATTGGCAAGACAAAACAGTAATGGTGGTCAGAAAAACAAGCC TCCTGAGCACGTTATAGACTGTGGAGACATAGTCTGGAGTCTTGCTTTTGGGTCTTCAGTTCCAGAAAAA CAGAGTCGTTGCGTTAATATAGAATGGCATCGGTTCCGATTTGGACAGGATCAGCTACTCCTTGCCACAG GATTAAACAATGGTCGCATCAAAATCTGGGATGTATATACAGGAAAACTCCTCCTTAATTTGGTAGACCA CATTGAAATGGTTAGAGATTTAACTTTTGCTCCAGATGGGAGCTTACTCCTTGTATCAGCTTCAAGAGAC AAAACTCTAAGAGTGTGGGACCTGAAAGATGATGGAAACATGGTGAAAGTATTGCGGGCACATCAGAATT GGGTGTACAGTTGTGCATTCTCTCCCGACTGTTCTATGCTGTGTTCAGTTGGCGCCAGTAAAGCAGTTTT CCTTTGGAATATGGATAAATACACCATGATTAGGAAGCTGGAAGGTCATCACCATGATGTTGTAGCTTGT GACTTTTCTCCTGATGGAGCATTGCTAGCTACTGCATCCTATGACACTCGTGTGTATGCCTGGGATCCAC ACAATGGAGACCTTCTGATGGAGTTTGGGCACCTGTTTCCCTCGCCCACTCCAATATTTGCTGGAGGAGC AAATGACCGATGGGTGAGAGCTGTGTCTTTCAGTCATGATGGACTGCATGTTGCCAGCCTTGCTGATGAT AAAATGGTGAGGTTCTGGAGAATCGATGAGGATTGTCCGGTACAAGTTGCACCTTTGAGCAATGGTCTTT GCTGTGCCTTTTCTACTGATGGCAGTGTTTTAGCTGCTGGGACACATGATGGAAGTGTGTATTTTTGGGC CACTCCAAGGCGAGTCCCTAGCCTTCAACATATATGTCGCATGTCAATCCGAAGAGTGATGTCCACCCAA GAAGTCCAAAAACTGCCTGTTCCTTCCAAAATATTGGCGTTTCTCTCCTACCGCGGTTAGACTGAAGACT GCCTTTCCTGGTAGGCCTGCCAGACAGAGCGCCCTTTACAAGACACACCTCAAGCTTTACCAGCTCAAGC GTCTGTCCTTGGAGGCTCAGCAGATTTATTTAATTGGAATCCACTCTTATTCTTCATTTCATTAGTTGGA CTTTTAAAGCATTATTTATACACCATAGAAATATTTCTGAACATATCATGTAATTTTTTTAAGATAACTG TGAAAACATTACATACATGTATATATTTAGACATGAGCTGCTGCTATATATTGAATGGGCCCTTTAGTTT TCTCTTAGTTCTGACCTGTATATATTGCTTTAATAGAGCCACGATTTGTATATGTGCTGTTCATTAGAGC AAATTCAGGGGAATTTTTGTTCTTTTTTTTTTATTTGGGGGCACTAAGTTTAGATGAGATGAATTGCCTC ATAGGAAATGGAATTGGTCAGGATAGATGGTGTGAAACTGATAAGAGGTTTTGGATGTGAAAAAGAGTTT CCAAAAGAATGGTGTCTGGGAGTTTGAATGTGATGATTAAAAAAAGAGCCAAGGAAAAAGGTAGCTAGAA AAAAAGGTTGGCTCTCATCAGCCTTAATTTTGGAAAGCAGCTACTTACCCCTCAGAGAATAAAGTATTAT GCATTCTTGTCAATTTGAAAACTGCATCTACTGTATTATTGCTGAATATTTTGTTAATACTAAGGGACAA TTATTTTAAGACCATGGGTTTTGTTTTTAACATATTTTATTTCTGCAGAGGATATTGGTAACTTACCAAA GAGAAGCAATACACAGTATGTCTGCTTTAGCCTTCTTTTATTTATCTCATCTGCAGATGTTGAGAATGTA TGCATTATGTAAAATGCTTGTTTTTTTATCCCTGTTGAGTTTTTTTAATTAAACGACACAAAACTAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30073116 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30073116 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005031 same experiment
	EMAGE:30641 same embryo
	EMAGE:31602 same embryo
	EMAGE:29954 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source