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EMAGE:30919

Dhx9 ( MGI:108177)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919
euxassay_010959_01 euxassay_010959_02 euxassay_010959_03 euxassay_010959_04 euxassay_010959_05
EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919
euxassay_010959_06 euxassay_010959_07 euxassay_010959_08 euxassay_010959_09 euxassay_010959_10
EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919
euxassay_010959_11 euxassay_010959_12 euxassay_010959_13 euxassay_010959_14 euxassay_010959_15
EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919
euxassay_010959_16 euxassay_010959_17 euxassay_010959_18 euxassay_010959_19 euxassay_010959_20
EMAGE:30919 EMAGE:30919 EMAGE:30919 EMAGE:30919
euxassay_010959_21 euxassay_010959_22 euxassay_010959_23 euxassay_010959_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
utricle
strong strong
regionalstrong expression: see section 03 04 05 06 07 17 18 19 20
vomeronasal organ epithelium
strong strong
regionalstrong expression: see section 12 14
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 17 18 19 20 21
metanephros
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 16 17 18 19 20 21
spinal cord
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 15 16
sublingual gland primordium
strong strong
regionalstrong expression: see section 09 15
rest of cerebellum marginal layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 13 14 15 16 17 18 19 20 21 moderate expression: see section 09 10
left lung
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12
cochlea
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 16 17 18 19 20
pancreas
strong strong
regionalstrong expression: see section 08 09 10 11 17 18 moderate expression: see section 12 15 16
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 19 20 21
lower jaw molar
strong strong
regionalstrong expression: see section 06 07 18 19
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 17
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 03 21 22 23 24
upper jaw molar
strong strong
regionalstrong expression: see section 06 07 18 19
thymus primordium
strong strong
regionalstrong expression: see section 10 11 12 13 14
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22
vibrissa
strong strong
regionalstrong expression: see section 05 06 07 08 09 19 20 21 moderate expression: see section 03 04 22 23 24
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
midbrain ventricular layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
right lung
strong strong
regionalstrong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09
brain
strong strong
regionalstrong expression: see section 01 02 03 17 18 19 20 21 22 23 24 moderate expression: see section 04 05 06 07 08 09 10 11 12 13 15 16
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 05 06 17 18
retina
strong strong
regionalstrong expression: see section 01 02 03 04 22 23 24
midgut
strong strong
regionalstrong expression: see section 09 10 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 11 12
thyroid gland
strong strong
regionalstrong expression: see section 09 10 14 15
submandibular gland primordium
strong strong
regionalstrong expression: see section 05 06 07 08 16 17 18 19
lower jaw incisor
strong strong
regionalstrong expression: see section 10 11 14 15
upper jaw incisor
strong strong
regionalstrong expression: see section 10 11 12 14 15 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36283
Entity Detected:Dhx9, ( MGI:108177)
Sequence:sense strand is shown

>T36283
AGGATGAGTGGAGAAGAAGCAGAAATACGTTTTTGTGAGCAGAAGAGACTCAATATGGCTACACTGAGAA
TGACTTGGGAGGCCAAAGTACAGCTTAAAGAGATTCTGATTAACTCTGGATTTCCCGAAGATTGTTTATT
GACACAAGTGTTTACTAACACTGGACCAGATAATAATTTGGATGTTGTTATCTCTCTGCTGGCTTTTGGT
GTGTACCCCAATGTATGCTATCATAAGGAGAAGAGAAAGATTCTTACCACTGAAGGGCGCAATGCACTTA
TCCACAAGTCATCTGTGAACTGTCCTTTCAGCAGCCAAGACATGAAGTACCCATCTCCTTTCTTTGTTTT
TGGTGAAAAGATTAGAACCCGAGCCATCTCTGCTAAAGGCATGACCTTAGTGACCCCCTTACAGCTACTT
CTCTTTGCCTCCAAGAAAGTCCAGTCGGATGGGCAGATTGTGTTTATAGATGACTGGATCAGACTGCAAA
TATCTCATGAAGCTGCAGCATGCATCACCATCCGGGCAGCCATGGAAGCTCTGGTTGTTGAAGTGTCCAA
ACAACCAAATATTATCAGCCAATTGGACCCTGTAAATGAACATATGCTAAACACAATCCGACAGATTTCT
AGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97644. Forward Primer - name:097644_F_cDNA_Dhx9, sequence:AGGATGAGTGGAGAAGAAGCAG; Reverse Primer - name:097644_N_SP6_cDNA_Dhx9, sequence:GCCTAGAAATCTGTCGGATTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_010959 same experiment
 EMAGE:31081 same embryo
 EMAGE:30910 same embryo
 EMAGE:31096 same embryo
 EMAGE:30832 same embryo
 EMAGE:29339 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS