EMAGE mouse gene expression spatial database

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EMAGE:31004

U2af1-rs1 ( MGI:98885)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000301_14	euxassay_000301_01	euxassay_000301_02	euxassay_000301_05	euxassay_000301_06

euxassay_000301_08	euxassay_000301_09	euxassay_000301_10	euxassay_000301_11	euxassay_000301_12

euxassay_000301_13	euxassay_000301_15	euxassay_000301_16	euxassay_000301_17	euxassay_000301_18

euxassay_000301_20	euxassay_000301_22	euxassay_000301_23	euxassay_000301_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

homogeneous

weak expression: see section 18

vagus x ganglion

moderate

homogeneous

moderate expression: see section 10 weak expression: see section 19

trigeminal v nerve maxillary division

moderate

homogeneous

moderate expression: see section 07

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 04 05 07 09 10 weak expression: see section 06 18 19

trigeminal v nerve mandibular division

moderate

homogeneous

moderate expression: see section 08

liver

moderate

homogeneous

moderate expression: see section 10 15 16 not detected expression: see section 19

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 10 weak expression: see section 19

testis

strong

single cell

strong expression: see section 08

otic capsule

weak

regional

weak expression: see section 05

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1693
Entity Detected:	U2af1-rs1, ( MGI:98885)
Sequence:	sense strand is shown >T1693 TGGCCTCGAGCCAGATTCGGCACGAGGCAANAAGCTACCACTCTGGTTCATACCACTCCTCCAAGAGAAA CAGGGAGTCCGAGAGGAAGAGTCCTCACAGGTGGAAGAAATCTCACAAACAGACAACGAAGAGTCATGAG AGGCACAGTTCAAGAAGAGGAAGAGAAGAGGACAGCAGTCCAGGTCCACAAAGCCAGAGCCACAGAACCT GAGCCCAGCTAAGGCAGCACCACTTGGACCCAAGACTGATATCAGGCTTGGCAAGGGAGTTCATTTTACA AATGAACAAAACTTCATTACAGAATAAAAACCTAACACAGTATCCTTCAGATTCCCCAAAATATTCAAAA CGAAACTGATTACAAAGGAAAGGCATGGTGACTCCATCTAATTCCCAACCAAGTTACAAAACCTTGTGGC TTTAAGAAACAGAAACAGGTATTCAGTATTTCTTTGTCTGTTATAATGCTTATAATGCTGTTATAATGCT TAGTATCATGTTATTGATGTTATTTTCCATACAGTTTAAGCTGGCTTCCCATGTTGTTTTCCTGTAATA
Notes:	The probe template was PCR amplified from IMAGE:476501 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:476501 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000301 same experiment
	EMAGE:29318 same embryo
	EMAGE:31935 same embryo
	EMAGE:31904 same embryo
	EMAGE:31045 same embryo
	EMAGE:31940 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source