EMAGE mouse gene expression spatial database

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EMAGE:31940

Meox1 mesenchyme homeobox 1 ( MGI:103220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000170_01	euxassay_000170_02	euxassay_000170_03	euxassay_000170_04	euxassay_000170_05

euxassay_000170_06	euxassay_000170_07	euxassay_000170_08	euxassay_000170_09	euxassay_000170_10

euxassay_000170_11	euxassay_000170_12	euxassay_000170_13	euxassay_000170_14	euxassay_000170_15

euxassay_000170_16	euxassay_000170_17	euxassay_000170_18	euxassay_000170_19	euxassay_000170_20

euxassay_000170_21	euxassay_000170_22	euxassay_000170_23	euxassay_000170_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

atrioventricular canal

strong

regional

strong expression: see section 13 14 15

atrioventricular cushion tissue

strong

regional

strong expression: see section 13 14 15

sternum

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1701
Entity Detected:	Meox1, mesenchyme homeobox 1 ( MGI:103220)
Sequence:	sense strand is shown >T1701 TGGCCTCGAGCCAGAATTCGGACGAGGCAAAGGACTGGGAAACTATAGAAACAGAGCCTGGGGCCCTCTT TTCTGGGTCTCTTGGCTACTACCCACACCTAGCATCCCCCTCTTACATCAGGGTCTCCTCTGGGTCTGGC CCCATCTGGCCTATGCAGAATCCATTCCTTGGTACCAGCTGGTGACTTGTAAAAGCAAACCTCTCTCCAG ATGTTCACACCGTTGAGTTGAAGGTTAGGAAGTGGCTCCTGTGTCTAAGGACGGAGAGGATAGCACAAGA GCTGATGGATGAGGACTGAGATACGTGGACCTTGGAAGTAGAACGTTCTCAGGCTGCAGCGTCTTGTGTT CTCCAAGGACTCTGACTGCAGATAAGGACAGGAAAACAACCCATCTTCCTCCATGGGATGCATTTGGACC TATCCTGCGTGTTCCGGAAAAAGCTTTGTGGGAAGACCTCCCAGGTTCACACACATGCGCAGCTCAGATC TCAGACCCAACTTCTGAGGATGCTCCTGTGAGGACTGGTGGGAAAACAGCCTCAGGCAACAG
Notes:	The probe template was PCR amplified from IMAGE:477164 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:477164 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000170 same experiment
	EMAGE:29318 same embryo
	EMAGE:31935 same embryo
	EMAGE:31904 same embryo
	EMAGE:31004 same embryo
	EMAGE:31045 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source