EMAGE mouse gene expression spatial database

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EMAGE:31904

Kcnj16 ( MGI:1314842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000169_01	euxassay_000169_02	euxassay_000169_03	euxassay_000169_04	euxassay_000169_05

euxassay_000169_06	euxassay_000169_07	euxassay_000169_08	euxassay_000169_09	euxassay_000169_10

euxassay_000169_12	euxassay_000169_13	euxassay_000169_14	euxassay_000169_15	euxassay_000169_16

euxassay_000169_17	euxassay_000169_18	euxassay_000169_19	euxassay_000169_20	euxassay_000169_21

euxassay_000169_22	euxassay_000169_23	euxassay_000169_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain floor plate

strong

homogeneous

strong expression: see section 12 13

early nephron

strong

homogeneous

strong expression: see section 10 11 12

spinal cord floor plate

strong

regional

strong expression: see section 14

renal cortex

strong

homogeneous

strong expression: see section 10 11 12

medulla oblongata floor plate

strong

homogeneous

strong expression: see section 13 14

metencephalon floor plate

strong

homogeneous

strong expression: see section 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1782
Entity Detected:	Kcnj16, ( MGI:1314842)
Sequence:	sense strand is shown >T1782 TGGCCTCGAGGCCAGAATTCGGATCCATGNTTTATTGCATATACCTGTGTCTCCTCTTGAAGGTGAAATA TTTAGGAGTGTGGGGAAAAGAGGCTGTTAAACTAGATAAATATAGAGAAGCAGATAAGCTAAATATTTGC ATATAAAAATAAATTAATTATGGAATGAGAACTTATAACCGTATTGTATCACCTAAGCACAGCAGGTGAA ATATGCCTCCGCTCAGAAACATTGAGATGAGAAAGTAAAACCAAGTGAAGTTTGAGTTTACCATATTTGC CTCTCATAGTATCTTGCAGACAGAGGTGTGGCTTTAGGAAAGTGAAAATGCTATCAAGTCAGAGATGCTT GCCTAAAGTGTTATTAGTGAATGCTGGAAGCCAGAACCAACCATGTGACAGAAGAAAAGGGACATCATTA CGTAAATTAGTATTTCTCTCTTAAATACCTTGGTTCAACCAAGCCACTGGGGAGCAGAAGGGAGGCGGTG GCCTTTCTGTGTGCTCCCTGGAGAAGGTTCCTGTGCTCATAATCCCACTGTG
Notes:	The probe template was PCR amplified from IMAGE:572121 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:572121 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000169 same experiment
	EMAGE:29318 same embryo
	EMAGE:31935 same embryo
	EMAGE:31004 same embryo
	EMAGE:31045 same embryo
	EMAGE:31940 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source