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EMAGE:31071

Mtap1b ( MGI:1306778)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31071 EMAGE:31071 EMAGE:31071 EMAGE:31071 EMAGE:31071
euxassay_009367_01 euxassay_009367_02 euxassay_009367_03 euxassay_009367_04 euxassay_009367_05
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 08 18
vomeronasal organ
strong strong
regionalstrong expression: see section 13 16
trigeminal v ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23
peripheral nervous system
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 06 07 08 09 18 19 20 21
spinal cord
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 18 19
trigeminal v nerve
strong strong
regionalstrong expression: see section 10 18 19
retina
strong strong
regionalstrong expression: see section 01 02 03 04 05
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36652
Entity Detected:Mtap1b, ( MGI:1306778)
Sequence:sense strand is shown

>T36652
CACCACAGGATTTGAACTGTGTTTCCAGAAATTCTTGAATTTGAAACTACCTTTTCTAAAACGTCCATTC
ATCCAATTAAGTCACTGAACAGTTATTACCTGCCAGATGCTATACTATGTCATGGTGATGCAAGTCACTG
ATAGTTCTCAATTTTTGCTGACTGCTAAGGGAAGTAACCATATTCCCACAATAGATTTCGAGTTACTGCA
AAATTGCCTACCCCCGTTCATCTCTGCTGAACATTCGGAAGCCATGCACTCACGCACCCAACTGACTTCT
GCTAGGTATTGGCATTTATCATAGAGAGAGAAAGAGAGGGAAAGAGAGTCAGCGGGAGGGGAGGGAGAGA
GGCGGGAGAGAGGGACAAAGAGACTGCAGGAGAGAGAGAGAGAGTGAGAGGGGAAGAATCAGAAAGAAAA
AGAATGCAAGAGACAAAGGTAGAGAGTTCTGATGAGATGCCCAGGGAGAGAGAGTGGGCAAGATGGGGTA
TAGAGAAGACACCAGCAACCAGGTCTGCATTTTCTCAGACCACAGCTATTCTGTGGTCTACACAGGTGGA
GTTTCCCATTTTCATCAGAGTCATCAGAAAGAGTCTTATTCTCCTAAAGCGTGTTTCTAAAGAACAAAAA
GAATGAAGCCTGTTACGAGGGCATGAGCTTTTTCACGCCTTAATTCAATGCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98154. Forward Primer - name:098154_F_cDNA_Mtap1b, sequence:CACCACAGGATTTGAACTGTGT; Reverse Primer - name:098154_N_SP6_cDNA_Mtap1b, sequence:AGGCATTGAATTAAGGCGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009367 same experiment
 EMAGE:32033 same embryo
 EMAGE:32034 same embryo
 EMAGE:30141 same embryo
 EMAGE:30453 same embryo
 EMAGE:31564 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS