Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:32033

Mmp25 ( MGI:2443938)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033
euxassay_009395_01 euxassay_009395_02 euxassay_009395_03 euxassay_009395_04 euxassay_009395_05
EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033
euxassay_009395_06 euxassay_009395_07 euxassay_009395_08 euxassay_009395_09 euxassay_009395_10
EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033
euxassay_009395_11 euxassay_009395_12 euxassay_009395_13 euxassay_009395_14 euxassay_009395_15
EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033
euxassay_009395_16 euxassay_009395_17 euxassay_009395_18 euxassay_009395_19 euxassay_009395_20
EMAGE:32033 EMAGE:32033 EMAGE:32033 EMAGE:32033
euxassay_009395_21 euxassay_009395_22 euxassay_009395_23 euxassay_009395_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
trigeminal v ganglion
strong strong
spottedstrong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36635
Entity Detected:Mmp25, ( MGI:2443938)
Sequence:sense strand is shown

>T36635
CACTTACTTTTTCAAGGGCACCCATTTCTGGCGCTTTGCGGAGGGCAGTGTCAAAGCTGAGTCAGATTCC
CCCCAGCCCATTGGGCCCAAGTGGCTGGACTGCCCTGCCCCCAACTCTGACCCTCGAGTCACCAGTCCCC
CGAAAACGACATCCAAAACCCGAAGCTGTGATTGTCACTGCGAGCTCAATCAAGCCTCAGAGCAGCTGTC
GCCCCTCCTGCTGCCTCTCCTACCCCTAGTGGCTGGAGAGGTCTTTTCTTACTGAAACCTGGTCCTTCCT
TGTGGACTTCCTATATAAAAGGAAGACGGAGTCTCCCGCAGCGGGGCCTCACCATCTGTGCTCCGCGTCC
CTCATCCCACGTGCACAGAGCTGAGAGATTCCTAGTAGAAGCCCGGGGACGCCTCCAGTGTTACTGAGTC
TGCTTTCTGTTAGGGTGCAGGGAGCCGTGGCCATAGTGAGGGTGGCCGCCAGGGGGTGGTGTGCACCCTC
TCCATCGCCAAGAGGGGCGTGCCTGCTGTAGCTACTAGAACTAGAAACCCAGCAGAATTGCAGCTCCTCA
AACCAGAATTGCTCCTCCAAACCAAGGTGACACTAACACCCAGGTTATGTGCTGCCTTCTGCTGAGCTAA
CTGGCTTTCTGGGAATCCCCACTCCTCTAATTCTCAACTAGTCCTACTGATCACAGTGGGATGGTGACTG
TACTCTGACTAGATGCGTTAAACAGGACCAGGACATTGAACCCCAGCATCTCCTTTAGCCTCTGAATGTT
CCTGAATCTTCTTACCATGATCTACTCCCTCCATGTCCCTTCATAATCACTGCCACAGGGTAGGGACTTA
GGCCAAGCACTGCCACCTCCTCTAGGGTGTGCGTCAAGTCTTGGCTGACAACAGTGTTGATGTGGATGGA
ATTTCTCCATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85211. Forward Primer - name:085211_F_cDNA_Mmp25, sequence:CACTTACTTTTTCAAGGGCACC; Reverse Primer - name:085211_N_SP6_cDNA_Mmp25, sequence:CATGGAGAAATTCCATCCACAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009395 same experiment
 EMAGE:31071 same embryo
 EMAGE:32034 same embryo
 EMAGE:30141 same embryo
 EMAGE:30453 same embryo
 EMAGE:31564 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS