EMAGE mouse gene expression spatial database

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EMAGE:31153

NP_TR6JSE50FPA ( MGI:3031206)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001136_01	euxassay_001136_02	euxassay_001136_03	euxassay_001136_04	euxassay_001136_05

euxassay_001136_06	euxassay_001136_07	euxassay_001136_08	euxassay_001136_09	euxassay_001136_10

euxassay_001136_11	euxassay_001136_12	euxassay_001136_13	euxassay_001136_14	euxassay_001136_15

euxassay_001136_16	euxassay_001136_17	euxassay_001136_18	euxassay_001136_19	euxassay_001136_20

euxassay_001136_21	euxassay_001136_22	euxassay_001136_23	euxassay_001136_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 17 18 19 20 21 22

hindgut

weak

homogeneous

weak expression: see section 14 15 16 17 18

foregut-midgut junction

weak

homogeneous

weak expression: see section 14 15 16 17 18

stomach

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 weak expression: see section 07 08 09 10 11 12 13

midgut

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4312
Entity Detected:	NP_TR6JSE50FPA, ( MGI:3031206)
Sequence:	sense strand is shown >T4312 GTTCAGAGTTCGCCCTAGGTCATTTAGCAGTTATTGTCACACAGCACTGCGGTATCCAGAGATGGGCAAC TTTCCTCGTGCTCAGTCCAACCTAAGACATGGGGCCCGGTGGCGATAGGGTTACCCTATGACGGGTCCTG CTGTTCCTGGAATCCAAGCCGACTGCAGAGTTTTTAAAACAAGGACTCCTCATGCTTTCAAACTGTGACA CCTAGCAGATTCAACATGAGATAGACCACAATTAAGGCAAATATGCCAGCGCTTCATAAAGCTTTGGGTT ACTTTGTGGGCTCAAATGACTTCAAAGTGCATGCTCTGATTGGAAGCATTGAGCTGTCTTCTGTTTTGTG AGAGTGACAATCTATGAGCAGCTTCAACACCGCCTTAGAAGGTGGCTTCATTTTGATGGGCTTCTCAGAC TGGCCTCATCTGGAACATATCTTTTTTGTCTTCATTTCAATGTTCTATTTCCTAACCATCTTTGGAAACT TCACCATCATCACAATTTCACGAATGGATCGTCGACTACAAACACCAATGTACTTCTTCCTTAACAACCT CTCTTTCCTGGACCTCTGCTACAACACCAGCATTGTACCTCAGCTTCTTGTCAATATTTCTGGAATTGAC AAGACCATGAGCTATGCTGGGTGCATGACCCAGTTCTTCATAGTGCTCTTACTGGGTGGAACTGAGTGTA TGCTCCTTGTGGTGATGGCTTTCGACCGCTATGTCGCTGTGTGTCATCCACTACACTACACCAGCATTAT GCACCCCCTTCTCTGCCATGCATTGGCCATCTCCTCCTGGGTGGGAGGCCTTGTGAACTCTCTGACTCAG ACAAGCCTCATCATGACCATACCTCTCTGTGGCCATCACCTGAACCACTTCTTCTGTGAGATGCTTGTTC TCCTGAAGCTGGCTTGTGAGGACACAGTGGGAACAGAGGCCAACTTGTTTGTGGCTGGAGCGATTATTTT GGTCTGTCCTGTAGCACTAATTCTAGGCACCTATGCACACATTGCTCATGCAGTGTTGAAGATCAAGTCA AGATCTGGGCGCAGAAAGGCTCTGGGGACTTGTGGGTCCCACCTTACTGTGGTTTTTCTTTTTTATGGCT CAGCCATGTACATGTATCTCCAACCTGTCCACGTGTATTCCGGGAGTGAAGGAAAGTTTGCTGCCCTCTT TTATACTATCATTACTCCAATGATGAACCCTGATTTATACCCTAAGAAACAAGAATGTGAAGGGGGCTTT GTGCAAGGTACTAGGGAGAGATACAAGCACAACATAGGAAGGGAACAGAACTTTATACAAATATTGTCTA ATATTTCTCGTATGACAAAACTCATCTTTCCTATAGAGACCTATTATTCTCCAGAAGATATTCCTCAGTA GGCATCATAATTGTACTTCTTACTTATCTAAAAATTAAAACTGGTGGTCTAAAACACTTCTAGATCCTCA CGAAATAGGTATTAAGGGTTGCTTTGTGTCATAACTTCTCCAAGGAATGAGTCTGGGAGAGAGGTATAGC GATTCTCCATAGGAGAATGGACATGGACTCTAAGTATAATTGAGAGTGAGAGAATGTAGGTGGTTGGTCT GGTGCTGCTTGTATTCAAATGTCAAATTCTGTATCGCAACACATGATTGCTCCAAGATGAGCAGATCTTC ATGTATACCAGCTTTTGTTGTAAACCTTGCCCCCCAAGTTATCCATGATTGGTTAATAAAGATGCCTACA ACCTGGAGTCGGCAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4507463 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4507463 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001136 same experiment
	EMAGE:32154 same embryo
	EMAGE:30949 same embryo
	EMAGE:32138 same embryo
	EMAGE:32139 same embryo
	EMAGE:31199 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source