EMAGE mouse gene expression spatial database

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EMAGE:31199

Hcn3 ( MGI:1298211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001127_01	euxassay_001127_02	euxassay_001127_03	euxassay_001127_04	euxassay_001127_05

euxassay_001127_06	euxassay_001127_07	euxassay_001127_08	euxassay_001127_09	euxassay_001127_10

euxassay_001127_11	euxassay_001127_12	euxassay_001127_13	euxassay_001127_14	euxassay_001127_15

euxassay_001127_16	euxassay_001127_17	euxassay_001127_18	euxassay_001127_19	euxassay_001127_20

euxassay_001127_21	euxassay_001127_22	euxassay_001127_23	euxassay_001127_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 11 12 13 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 weak expression: see section 10 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 21 22 23 24 weak expression: see section 10 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 weak expression: see section 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 weak expression: see section 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4317
Entity Detected:	Hcn3, ( MGI:1298211)
Sequence:	sense strand is shown >T4317 GGCCCTGGACCGGTTGGGGAGGCCCTACGAGTGAGGGCAATCATGGAGGAGGAGGCGCGGCCGGCGGCGG GGGCCGGCGAAGCGGCGACCCCTGCACGCGAGACGCCTCCTGCGGCTCCGGCCCAGGCCCGCGCGGCCTC AGGTGGGGTGCCGGAGTCTGCGCCCGAGCCGAAGAGGCGGCAGCTCGGGACGCTGCTGCAGCCGACGGTC AACAAGTTCTCTCTCCGGGTCTTCGGCAGCCACAAAGCAGTAGAAATCGAGCAGGAGAGGGTGAAGTCCG CCGGGGCCTGGATCATCCACCCCTACAGCGACTTCCGGTTTTACTGGGATCTCATCATGCTGCTGCTGAT GGTGGGGAACCTCATAGTTCTGCCTGTGGGTATCACTTTCTTCAAGGAGGAGAACTCTCCACCCTGGATC GTCTTCAATGTCCTCTCTGACACTTTCTTCCTGCTGGATCTGGTGCTCAACTTCCGAACTGGCATCGTGG TGGAGGAAGGTGCCGAGATCCTGCTGGCGCCAAGGGCCATCCGAACGCGTTACCTGCGCACCTGGTTCCT GGTTGATCTGATCTCCTCCATCCCTGTGGATTATATCTTCCTAGTGGTGGAGCTGGAGCCACGACTAGAT GCTGAGGTCTACAAAACGGCACGGGCCCTGCGCATCGTTAGATTCACCAAGATCCTTAGCCTGCTGCGGC TGCTCCGCCTCTCCCGCCTCATCCGCTACATACACCAGTGGGAGGAGATCTTTCACATGACCTACGACCT GGCCAGTGCAGTGGTTCGCATCTTCAACCTCATTGGAATGATGTTGCTGCTGTGTCACTGGGACGGCTGT CTGCAGTTTCTGGTCCCTATGCTGCAGGACTTCCCGTCCGACTGCTGGGTCTCCATGAACCGCATGGTGA ACCACTCGTGGGGCCGCCAGTATTCCCACGCCCTGTTCAAGGCCATGAGTCACATGCTATGCATTGGCTA TGGGCAGCAGGCACCGGTAGGCATGCCTGACGTCTGGCTCACCATGCTCAGTATGATTGTGGGCGCCACG TGTTATGCCATGTTCATCGGTCACGCCACCGCCCTCATCCAGTCCCTGGACTCTTCCCGGCGACAGTACC AGGAGAAGTACAAGCAGGTGGAGCAGTACATGTCCTTCCACAAGCTGCCCGCTGACACCCGGCAGCGCAT CCACGAGTACTACGAGCATCGCTACCAGGGCAAGATGTTTGATGAAGAGAGCATCCTGGGGGAGCTGAGC GAGCCACTTCGGGAGGAGATTATTAACTTCACCTGCCGGGGCCTGGTGGCCCACATGCCGCTGTTTGCTC ATGCTGACCCCAGCTTCGTCACCGCAGTGCTCACCAAGCTCCGTTTTGAGGTCTTCCAACCAGGGGACCT GGTGGTGCGTGAGGGCTCCGTGGGCAGGAAGATGTACTTCATCCAGCACGGGCTGCTGAGTGTGCTGGCA CGTGGCGCCCGCGACACCCGCCTCACTGATGGATCCTACTTTGGGGAGATCTGCCTGCTGACTCGAGGTC GGAGAACAGCCAGTGTAAGGGCTGACACCTATTGTCGCCTCTACTCGCTCAGCGTGGACCACTTCAATGC GGTGCTTGAGGAGTTCCCAATGATGCGCAGGGCTTTTGAGACGGTGGCCATGGACCGGCTTCGGCGCATC GGCAAAAAGAATTCGATACTGCAGCGGAAACGCTCTGAGCCGAGTCCAGGCAGCAGCGGTGGCGTCATGG AGCAGCATTTGGTACAACACGACAGAGACATGGCTCGTGGTGTTCGGGGCCTGGCTCCTGGTACAGGAGC TCGACTCAGTGGAAAGCCAGTGCTGTGGGAACCACTGGTGCACGCCCCTCTGCAGGCAGCTGCTGTGACC TCCAACGTGGCCATAGCCTTGACTCACCAGCGAGGCCCTCTGCCCCTCTCCCCTGATTCTCCAGCCACCC TCCTAGCTCGATCTGCTAGACGCTCAGCAGGCTCCCCAGCCTCCCCACTGGTGCCTGTCCGAGCAGGTCC TCTGCTGGCCCGGGGACCCTGGGCGTCCACTTCTCGCCTGCCTGCTCCACCTGCCCGAACCCTCCATGCC AGCCTATCCCGGACAGGGCGTTCCCAGGTATCTCTGTTGGGCCCTCCCCCAGGAGGAGGTGCTCGGAGGC TAGGACCTCGGGGCCGCCCACTTTCTGCCTCGCAACCCTCTCTGCCTCAGCGAGCAACAGGGGATGGCTC TCCTAGGCGTAAAGGCTCTGGAAGTGAGCGCCTGCCCCCCTCTGGGCTCTTGGCCAAACCTCCAGGGACA GTCCAGCCACCCAGGTCATCAGTGCCTGAGCCAGTTACCCCCAGAGGTCCCCAAATTTCTGCCAACATGT GAAATCCTAGGTTACACTGGCCTTAGCTATTGGCACGCAATAACGTGTGCCCGAGGGTAGACGCCCCTTG GGGGAAGGCTGTGGGGGACCTAACATACTGCCCTTTATCACCACAAAGCTATTCTCTGTGTCCTCAGCTC AAAGCAGCTGCAGCCTGCTAATCCTAATCACATACCCATTTGTCATTGGTACCCCATGCCTACCATGTTC AAGGCACTGTGCTAGGTACTGTGTATCCACTGCCAAGTAGAAATAACTCGAAGCCCTTTAACAAGGGTGT CCCCAGGCCACGGTCCTGCCAGGCGCAGGCCTGGGCCCACAGCCCTGCCTTTATTAAGCACAAGTACTTG CTGCCTCCATCACTGCCGAGTAACTAGACGGCTGTCTCCCTGTCAGTGACCCTGCAGGTGGTGCCAGTGC GTCCTCTCTTCCCATCTCCTTTGTCGTAGCTGGGTACCGTCAATTATCTAGTACAGCCAACAGATGTCTA GAGCCCTGGGTGTGAACATCGACCCTCTGACTGGCACCTGTGGGCCCTCCTTGTCCACACCAAGTGAGAA TCCACGTCTGCCCACTGTAGTCTGCCCTCCGCAGCTCCTGTGTGGACGCACTACCTTTATGAGCTAGAAG CTGCTCTTCACTGTGCCCCCTGCCTCAGTGGTGTTGCCCCTAAAGCTAAGGGGCACGCGGCACCTCCTTA CCTACTGTGCCAGCCTAGTTCCCCCCAGGTAGGGGGCCAGGGGCTGAATCCTCATGTGACCTTTTATCTT TCCCTCTTTGTCTTATTTATTTGGTCGTTTATTAATTTTATTTATTCATTTGCTAATTTGAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4511289 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511289 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001127 same experiment
	EMAGE:32154 same embryo
	EMAGE:31153 same embryo
	EMAGE:30949 same embryo
	EMAGE:32138 same embryo
	EMAGE:32139 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source