EMAGE mouse gene expression spatial database

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EMAGE:32139

0610031J06Rik ( MGI:1913318)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001077_01	euxassay_001077_02	euxassay_001077_03	euxassay_001077_04	euxassay_001077_05

euxassay_001077_06	euxassay_001077_07	euxassay_001077_08	euxassay_001077_09	euxassay_001077_10

euxassay_001077_11	euxassay_001077_12	euxassay_001077_13	euxassay_001077_14	euxassay_001077_15

euxassay_001077_16	euxassay_001077_17	euxassay_001077_18	euxassay_001077_19	euxassay_001077_20

euxassay_001077_21	euxassay_001077_22	euxassay_001077_23	euxassay_001077_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

posterior semicircular canal

weak

homogeneous

weak expression: see section 08 09 19 20 21

cochlea

weak

homogeneous

weak expression: see section 07 08 09 10 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4308
Entity Detected:	0610031J06Rik, ( MGI:1913318)
Sequence:	sense strand is shown >T4308 GCAGAGTTGAACTAGCTGTGGCTGTATGTTTCGCTGTTGGGGACCTCACTGGGGCTGGGTCCCCTGTGCC CCTACCCCATGGCTCCTTTTGAGTCTACTTGTGTGTTCAGCTCCATTTGGCCTGCAGGGAGAAGAGACTC GCCAGGTATCTATGGAGGTTATTTCCGGCTGGCCAAATCCACAGAACCTGCTTCATATTCGGGCAGTGGG CAGCAACTCCACCCTGCACTATGTGTGGAGCAGCCTGGGGCCTCCAGCTGTGGTGCTGGTGGCCACTAAC ACCACTCAGAGTGTCCTGAGTGTCAACTGGAGTCTCCTGCTGTCTCCCGATCCTGCTGGGGCCCTGATGG TGCTCCCCAAGAGCAGCATACAGTTTTCTTCTGCTCTCGTCTTCACCAGGCTGCTTGAGTTTGACAGCAC CAATGCGTCCGAGGGGGCACAGCCTCCAGGAAAGCCATACCCTCCGTACTCCTTGGCCAAGTTCTCCTGG AACAATATCACCAACTCATTGGATCTTGCCAACCTGAGTGCCGATTTTCAAGGCCGCCCTGTAGATGATC CTACTGGAGCATTCGCCAATGGCAGCCTGACCTTCAAGGTCCAAGCCTTTTCTAGATCTGGTCGACCTGC TCAGCCCCCTCGCCTCCTGCACACAGCAGATGTCTGCCAGTTAGAAGTGGCCCTGGTTGGAGCTTCTCCT CGTGGAAACCATTCCCTATTTGGTCTAGAGGTAGCCACCTTGGGACAGGGCCCTGACTGCCCCTCAGTGA ACGAACGGAACTCCATCGATGATGAATATGCACCTGCCGTCTTCCAGCTGAACCAGCTGTTATGGGGCTC TTCTCCATCTGGCTTCATGCAATGGCGACCAGTGGCTTTCTCTGAGGAGGAGCGGGCCCGAGAATCAGCC CTCCCCTGCCAGGCTTCCACTCTTCACTCCACCTTGGCATCTTCTCTCCCTCATTCACCAATTGTTCAAG CCTTCTTCGGGTCCCAGAACAACTTCTGTGCCTTCAATCTGACCTTTGGGGCTCCCACGGGCCCTGGCTA CTGGGACCAATACTACCTTTGCTGGTCAATGCTTCTGGGCATGGGCTTCCCTCCGGTGGATATCTTCTCT CCACTAGTCCTCGGAATCATGGCGGTAGCCCTGGGAGCCCCAGGACTCATGTTCCTGGGGGGAGGCCTAT TTCTGCTGCTGCGCCACAGGCGGTATTCTGAGTACCAGTCCATAAACTGAAACCCACTCTCCAGAGGGAA GGGCAGTGTCTCAGACTGTCGGAGGGTGGGGGGCCCAGGGTCATGATCTGCTTTTCTGCAGAACCTCAGA AGCCAGCCTCAACCTACTGGGGTGCTCCCAGTAAGTTGTCCTTCCAAACCCACGGAGAGGCCACGCTCAC GGGTTCCCAAACTCCCGTTTCCTGTGGATGAAACTTGAAGGCCTACATGAGTGACTGTTGGCTGCCCTAA GGGGCATGAAATAGACATTTCCCCCATACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4507218 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4507218 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001077 same experiment
	EMAGE:32154 same embryo
	EMAGE:31153 same embryo
	EMAGE:30949 same embryo
	EMAGE:32138 same embryo
	EMAGE:31199 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source