EMAGE mouse gene expression spatial database

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EMAGE:31155

Trappc6b ( MGI:1925482)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006829_01	euxassay_006829_02	euxassay_006829_03	euxassay_006829_04	euxassay_006829_05

euxassay_006829_06	euxassay_006829_07	euxassay_006829_08	euxassay_006829_09	euxassay_006829_10

euxassay_006829_11	euxassay_006829_12	euxassay_006829_13	euxassay_006829_14	euxassay_006829_15

euxassay_006829_16	euxassay_006829_17	euxassay_006829_18	euxassay_006829_19	euxassay_006829_20

euxassay_006829_21	euxassay_006829_22	euxassay_006829_23	euxassay_006829_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 09 10 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 21 22 23

ventral grey horn

moderate

regional

moderate expression: see section 11 16 17 weak expression: see section 12 13 14 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v nerve

moderate

regional

moderate expression: see section 10 11 18

thoracic ganglion

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7116
Entity Detected:	Trappc6b, ( MGI:1925482)
Sequence:	sense strand is shown >T7116 GTGGGGCTGTCACAGGGTCTCTAGGGCCCAGTGGCGGCGGCCCCGGGGAGGGGCGCGGTCCTCGGCGGCC TTCCGCGGCCTCAGGACCGGGGTAGGCCGCTGAGCACGGCGTGCGGTGGGCGCGGGGTAACGGGAACCTC GAGTCCCGCACTGATCGGAGCCGACTCGGCGCTGGAGGGATCGGCGGGAAATGGCGGACGAGGCGTTGTT TTTGCTTCTCCATAACGAGATGGTGTCCGGAGTGTACAAGTCCGCCGAGCAGGGGGAAGTGGAAAATGGA AGGTGTGTTACTAAGCTGGAGAGCATGGGGTTCCGAGTGGGGCAAGGACTGATAGAAAGGTTTACGAAAG ATACTGCAAGGTTCAAGGATGAATTAGACATCATGAAGTTCATCTGTAAAGATTTTTGGACTACAGTATT CAAGAAACAGATTGACAATCTGAGGACAAATCATCAGGGCATATATGTACTTCAGGACAACAAATTTCGA CTACTCATTCAGCTGTCTGCAGGAAAACAGTATTTAGAACATGCGTCCAAGTATCTAGCATTCACATGTG GCTTAATCAGAGGTGGCTTGTCGAACTTGGGAATAAAAAGTATTGTAACAGCTGAAGTCTCTTCAATGCC TGCCTGCAAATTTCAGGTGATGATACAGAAGCTGTAGAGCGAGCTGGCGACTCCTGGGCAGAGCGTTGCA CATCCTGATTTCACCTCATCGTTGGTTATCTGCGTTGGAGCAAATCGTTATCTCACCAGAGTCACATAGA CCAAATCAAAGTAGACACAGGTCCTTTGACCATGACAGAAACTGACGGACCTATTCAGTGATTTCAGAGA ACTAGACACCAAGATGCAAGGCTCTTCATTTTAAACATCTTTATTTCCATAGGTGATTAGCATTTAACCA TCAATAGGAAGTGAAACTCAGGGTGTGTTGAATTGCTGTATTAAAAAAATGCACCAATCAGAATAGTTTG TGTTCAAATGACCAAAATTTGTTGAACTATAAATCTGTTTTAGTTATTTAAAAAAGCAAACCACTATGTT AAATTAAGCTTAATTTTTATTGTATTGCTTATAATTTATTTCTGTCGAGAGAATTCATATGCTTATGTAA GGATATCATTTATACATTGTGTATATATGTGTATATATAAATACATGCATTACTGTCTAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:4017482 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4017482 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006829 same experiment
	EMAGE:31480 same embryo
	EMAGE:29506 same embryo
	EMAGE:31484 same embryo
	EMAGE:31366 same embryo
	EMAGE:31159 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source