EMAGE mouse gene expression spatial database

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EMAGE:31366

Snai2 snail homolog 2 (Drosophila) ( MGI:1096393)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006888_01	euxassay_006888_02	euxassay_006888_03	euxassay_006888_04	euxassay_006888_05

euxassay_006888_06	euxassay_006888_07	euxassay_006888_08	euxassay_006888_09	euxassay_006888_10

euxassay_006888_11	euxassay_006888_12	euxassay_006888_13	euxassay_006888_14	euxassay_006888_15

euxassay_006888_16	euxassay_006888_17	euxassay_006888_18	euxassay_006888_19	euxassay_006888_20

euxassay_006888_21	euxassay_006888_22	euxassay_006888_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

strong

regional

strong expression: see section 13 14

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20 21

rectum

strong

regional

strong expression: see section 13 14

midgut

strong

regional

strong expression: see section 13 15 16 17 18 19 20 21

midgut loop

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7957
Entity Detected:	Snai2, snail homolog 2 (Drosophila) ( MGI:1096393)
Sequence:	sense strand is shown >T7957 CAGGGAGCCGGGTGACTTCAGAGGCGCCTGCCTGTCCCCCGCCGCACCTGAGCCACCGCGATGCTATAGG ACCGCCGCCTGGACCGTTATCCGCCCGCGCCCGCCCGCAGCCACCATGCCGCGCTCCTTCCTGGTCAAGA AACATTTCAACGCCTCCAAGAAGCCCAACTACAGCGAACTGGACACACACACAGTTATTATTTCCCCATA TCTCTATGAAAGTTACCCTATACCTGTCATACCAAAACCAGAGATCCTCACCTCGGGAGCATACAGCCCT ATTACTGTATGGACATCGTCGGCAGCTCCACTCCACTCTCCTTTACCCAGTGGCCTTTCTCCTCTTACTG GATACTCCTCATCCTTGGGGCGTGTAAGTCCCCCGCCTTCCTCTGACACTTCATCCAAGGATCACAGTGG TTCAGAAAGTCCCATTAGTGACGAAGAGGAGAGACTGCAGCCCAAGCTTTCAGACCCCCATGCCATCGAA GCTGAGAAGTTTCAGTGCAATTTATGCAATAAGACCTATTCTACGTTCTCTGGGCTGGCCAAACACAAGC AGCTGCACTGTGATGCCCAGTCTAGGAAATCGTTCAGCTGCAAGTACTGTGACAAGGAATATGTGAGCCT GGGTGCCCTGAAGATGCACATTCGAACCCACACATTGCCTTGTGTCTGCAAGATCTGTGGCAAGGCTTTC TCCAGACCCTGGCTGCTTCAAGGACACATTAGAACTCACACTGGGGAAAAGCCTTTCTCTTGCCCTCACT GCAATAGGGCTTTTGCAGACAGATCAAACCTGAGGGCACATCTGCAGACCCACTCTGATGTAAAGAAATA CCAGTGCAAAAACTGCTCCAAAACCTTCTCCAGAATGTCGCTTCTGCATAAACATGAGGAGTCTGGCTGC TGTGTGGCACACTGAGTGGCGCAACCAGTGTTTACTCAAACAGAATGCATTTCTTCACTCCAATGACAAA TGACAAATGAAAGTCCAAAGACATTTTCTCATGTGCTTACCAACCAAATAGTATGTATAAAACCACAAAA GAGTCACACACACACACACACACACACACACACACACACACACAGAGAGAGAGAGAGAGAGAGAGAGAGA GACAGACAGACAGACAGACAGATACACACACACTACAGAACAGAATCTATGTACTTAAAGTTAATTCGTT CTATGTGAAGTTTAAAATTATATTTACTGACAGCTAGATTGAAAGGATAAAAGATAAGAATCTTTCTCTT TAAAGATGAAGTGAAAAGCACATTGCATCTTTTCTTACTAAGAAAGAATACAGAGATTTACACTGCTGCC AAACCATTTCAACCAAAGGAACAGTATTTCTTCTTAATAGAATTGTAATAGTGTTTCCAAGAGGAAGAGA GTCTGCCAGACACTATCTCAGGTGCCTTATAAAGTACTCCAAGTTTACTTCCTTAAATGTATGATGCCTG GTTGTCATCAGTGAATGACAGCCTTTTCTGGATTACCTACAATGTTTTAAAACTATATTGTTAAGAGAAA AAAAACCAAAAACAAGAAAAAGAACAGAACACAAGAGAATGTATTAAAGTATTCTTGTTTTATTTTTGCC ATGTGTGCCTTGGAAGAGGAGGGAAAGACAAACTTCAAACATTCCTGGTGCGTGTCCCATGTCTTTCTTT TTAAAAAAGAATCTTAATGTTTTATAATACAAAGTAATGAAAATGTGCAAAAGAATTTCTTAGACATTCA GTAATGTACTTAGACTTTTGAAAATTCATGTGATGGATGCAGTAATACAATGCCCCTCCAAGTGCCTGTC TTAATGACTTGTGTAGTTGATGAACTGATGTAAATTTGTGTTTATTTTTATACAACTGAATGAACTCTGT ATGAAAGTGAGGTACGGTTAATAGCCACGCCTATATTCAACCAGAATACTTGTGAAATCAATGTCCTTTT TTAAAAAGTAACTTTCAAGGTCTCTTTTTTACAATAAACATTTTTGAGTAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30242610 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30242610 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006888 same experiment
	EMAGE:31480 same embryo
	EMAGE:29506 same embryo
	EMAGE:31484 same embryo
	EMAGE:31155 same embryo
	EMAGE:31159 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source