EMAGE mouse gene expression spatial database

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EMAGE:31178

Zfp14 zinc finger protein 14 ( MGI:99160)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006835_01	euxassay_006835_02	euxassay_006835_03	euxassay_006835_04	euxassay_006835_05

euxassay_006835_06	euxassay_006835_07	euxassay_006835_08	euxassay_006835_09	euxassay_006835_10

euxassay_006835_11	euxassay_006835_12	euxassay_006835_13	euxassay_006835_14	euxassay_006835_15

euxassay_006835_16	euxassay_006835_17	euxassay_006835_18	euxassay_006835_19	euxassay_006835_20

euxassay_006835_21	euxassay_006835_22	euxassay_006835_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 15 16 17

vagus x ganglion

weak

regional

weak expression: see section 07 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 04 05 06 07 16 17 18 19 20 21

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06 16 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 06 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9230
Entity Detected:	Zfp14, zinc finger protein 14 ( MGI:99160)
Sequence:	sense strand is shown >T9230 GGCTGCTTAGCGCCGGTCCGCTCTGAGAGCAGAAGCCAGGAGAAGGTGTCTGATTCCCAGGAACTGGAGT TATAGACGGTCGTGAAACACCATCGTTGCTTGTTGACCCCCAGTTTATCACAAAGAAATACTGAAAAGAG CCGACCGGTTCCTGCAGTTCTAACACTATGGCCCTGGCTCAGGGTTTAGTGACGTTTGGGGATGTGGCTG TGGATTTCTCTCAGGAGGAGTGGGAATTCCTGGACCCTGCTCAGAAGAACCTGTACAGGGATGTGATGTG GGAGACCTATAGCAATTTTATTTCACTGGATTTGGAGTCTAGATTCAAGACCGATACTTCATCTTCAGAC AAGGGCATTTGTGAAGTATATTCGTTACAGTGGGAGTTGATTGAGAAAATTAAAAACCTAAGCCCTCAGG GTTCTGGTCTTAGTGATGATCAGGAATGTAAACACAAGACTGGGCTACAAAAGGAACCGCAGGAGGGTTA TTTTGGGCAGTTGAAAATTACCTCTGAAAAAGTCACATATGAAAAGCACAGTTTCCTTTCTGAGTACCAG AGAGTTCAGAATGGAGAAAAGTTCTATGAATGTAAAGAGTGTAGGAAGACCTTTATCCGCCGCTCAACGC TTAGTCAGCACTTGAGGATCCACACCGGCGAGAAACCTTACAAATGCAAGGAGTGTGGGCAACCCTTCAG ACAGCGGGCGCACCTTATCCGACATCACAAACTTCACACCGGTGAGAAGCCCTATGAGTGTAAGGACTGT GGGAAGGCCTTTACAGTGCTCCAGGAACTCACACAGCACCAGCGTCTTCACACTGGTGAGAAGCCGTACG AATGCAAGGAATGTGGAAAGGCCTTCCGGGTCCATCAGCAGCTGGCTCGGCATCAGAGGATTCACACTGG GGAAAAGCCCTATGAGTGCAAGGAGTGTGGGAAGACCTTCAGACAGTGCACACACCTTACCCGACACCAG AGACTTCACACTTCTGAGAAACTCTATGAATGTAAGGAGTGTGGGAAAGCCTTCGTGTGTGGCCCAGACC TTCGAGTACATCAGAAAATCCACTTTGGTGAGAAGCCCTATGCCTGTAAGGACTGTGGTAAGTCCTTCAG AATATGCCAACAGCTGACCGTCCACCAGAGTATTCACACTGGGGAGAAACCCTATGAATGTAAGGAATGT GGGAAGACCTTCAGATTGAGGCAGCAGCTTGTTCGCCATCAGAGAATCCATACCCACGAGAGACCCTATG AGTGTCTGGAATGCTGGAAGACCTTCAGTAGCTACTCCCAGCTGATTTCACATCAGAGCATTCATGTTGG GGAGAGACCGTACGAGTGTGAAGAGTGTGGGAAGGCCTTCCGGCTGCTCTCGCAGCTCACTCAGCACCAG AGCATCCATACAGGCGAGAAGCCTTATGAATGCCAGGAGTGTAGAAAACCCTTCCGGCTGTTGTCACAGC TCACTCAGCACCGGAGCATCCACACCGGCGAGAAGCCTTATGAATGCAAGGACTGTGGCAAGGCTTTTAG ACTTTATTCATTTCTTTCTCAGCACCAGAGGATCCACACTGGTGAGAAGCCCTACAAATGTAAGGAGTGT AAGAAGGCCTTCAGACAGCACTCACACCTGACCCAGCACCAGAAGATCCACAGTGGAACCTGACAAGCTC TACACCAAGTTGTAACATGTCAGGAAGTCCGTTTTTAAGAATAATTTATTTTGTGCTTGTAAGTCAGCAT AAATAAGACTGTGTTGCTTTAAAAGCCCACCATCCCCACTTAAATGTTACATTTCTTCAGCAAATTCCCA TGAAAGAATTTTGATGAATGTAGGGAAACGTTTAGCCTCATCTGTTATCATCTACATCTCACCACTCTCT TCCAGTGTGACACTGGACAGGACCCTAAGCATTAATGCCTTGTTTTGCTCACTGTCAAAATGGTGACAAT AGCACCTACACATATTATTTATCATTATGTAACAAATTAAATAGAAAAACAGTAGATTAAATAATACACT TGTATTATCTAAAAATTTCTATAGATCAAGAGTCCAGGTATGCCTTAGTTGGGTCTTTGAGGTTTCAGCT AAGGCTGGCTCCCATCCGAAAGCTTAGCTAGAGAAGGATCCACATTGGAGGGAACAGGTTAGACACCTGA ATTCATTTCCTTAAGAGCTGATCCACTGGGATTCTCAGTTTCTGGATGGCCATTGGCTGGAAGATGAGTG AAGCTCCTAGCCATATTGGCTGTCCAGTGTGGCTGCTGCTCTAATGATACTGCACAAGCCAAGAAAGGTG GCTGCTAGCAGGATGGAGGCCAGCTAGCCAGACTGACGTGATAATCTGTGCCGTCTAATCACAGAAGGAA CATCCAGTCATTTTACCACATTCTACTGGTAAAATGGAGTTATCAGACCAGCTTACTCCAGAAGTCTCGT GAAATTGAATGTCACAAGGAGGCTGGTGAGTGAGGCCCAGGGTAGAGCCCACCTGCTGTACTGCACAACA GCACTGACTTAAGGATAAAGTAGTTAACACTTGACCAACTAGCAGTCATGGTAGCATGTATCATACAATA ACGTCTGTAATTCTTACTGCCAGTATTGTCAATTCATGTGAAAGGAAGATGATCCCATGGAAGTAATAAA TGTAGAAATGCCTCCTTTCACATCTCTGCATTTATAAACCTCAACATAATTTCTCCTGGGTAAGACCTAG GAAATTATAGGAAATGGGAGATTTTTGTCACTTGAGCAAAAACTTCATGTTGAAAGGAGACTGAGTTTCA TGAATGTGTGTTATTAAAACTTAACTATTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6833833 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6833833 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006835 same experiment
	EMAGE:31418 same embryo
	EMAGE:32001 same embryo
	EMAGE:31448 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source