EMAGE mouse gene expression spatial database

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EMAGE:31448

C77668 ( MGI:2140050)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006847_01	euxassay_006847_02	euxassay_006847_03	euxassay_006847_04	euxassay_006847_05

euxassay_006847_06	euxassay_006847_07	euxassay_006847_08	euxassay_006847_09	euxassay_006847_10

euxassay_006847_11	euxassay_006847_12	euxassay_006847_13	euxassay_006847_14	euxassay_006847_15

euxassay_006847_16	euxassay_006847_17	euxassay_006847_18	euxassay_006847_19	euxassay_006847_20

euxassay_006847_21	euxassay_006847_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9177
Entity Detected:	C77668, ( MGI:2140050)
Sequence:	sense strand is shown >T9177 CAAAAGCTTTGCCCCAGCCCGGCCACTCCTGAGGGAGCATCTAGAGAACCCGCGCCTTCCCCCTCCGCAG GAGAGGAGGCAAGAGTGCGGAAAGCCAGGCCTGTCGGCCTCCCTCCCACTTGTTCCTCTTGCTCCTCGTC CTTGGCAAGCCAGAGTTCTCCATTTCTAGGACTTAACACCCGTCTACTCCCTTGACCTCTTCTGGTCTCT TGGCTGCAGCCATGGAGTTACAGAAGGGAAAGGGGACCGTAGCAGCTGCTGCATCAGGAGCAGCGGGAGG TGGAGGAGGAGGAGCGGGAGCAGGAGCCCCAGGAGGGGGGAGGCTGCTACTTTCAACCAGTTTGGATGCC AAGGATGAGTTAGAGGAGAGGTTGGAAAGGTGCATGAGTATTGTCACATCGATGACCGCTGGTGTCTCCG AGAGAGAGGCCAATGATGCTCTCAATGCCTATGTGTGCAAAGGACCTCCCCAGCATGAGGAGATCTGCCT AGGCCTGTTTACTCTTGTCCTCACTGAGCCTGCCCAAGCCCAGAAGTGTTACCGGGACTTGGCTCTGGTG AGTCGTGATGGTATGAACATCGTCCTGAACAAGATCAACCAGCTACTTATGGAGAAGTACTTGAAGCTGC AAGATACCTGCCGTACTCAGTTGGTGTGGTTGGTTCGGGAACTAGTAAAGAGTGGAGTTCTGGGAGCTGA TGGCGTTTGCATGACATTTATGAAGCAGATTGCAGGTGGCGATGTTACAGCCAAAAATATCTGGTTGGCA GAAAGTGTCCTGGATATCCTGACGGAGCAGAGGGAGTGGGTGCTGAAGAGCAGCATCCTCATTGCCATGG CTGTGTACACGTACCTCCGACTTATTGTGGACCACCATGGGACTGCCCAGCTCCAGACCCTTCGGCAGAA GGAAGTTGACTTCTGCATCTCCTTGCTTCGGGAACGGTTCATGGAATGTTTGATGATTGGCCGGGACCTT GTAAGATTACTTCAGAATGTTGCTAGGATACCAGAATTTGAACTACTGTGGAAAGATATCATCCATAACC CTCAGGCCTTGAGTCCTCAGTTTACAGGTATCCTACAGCTACTTCAGTCAAGAACATCCCGAAAATTTCT AGCCTGTCGTCTAACCCCAGACATGGAAACGAAGCTCCTCTTCATGACATCCCGGGTGCGGTTTGGCCAG CAAAAGCGGTACCAGGACTGGTTCCAGCGCCAGTACCTCTCAACCCCTGACAGTCAGTCTCTGCGCTGTG ACCTCATTCGATACATCTGTGGGGTAGTCCACCCCTCTAACGAAGTGCTGAGTTCTGATATCTTGCCCCG GTGGGCCATCATTGGCTGGCTCCTGACAACATGCACGTCCAACGTTGCTGCCTCCAATGCCAAGCTGGCT TTGTTTTATGACTGGCTGTTCTTCAGCCCAGAAAAGGATAGTATTATGAACATAGAGCCAGCTATCCTGG TCATGCATCACTCCATGAAGCCCCACCCAGCCATCACTGCCACGCTCCTGGACTTCATGTGCCGAATCAT TCCTAACTTCTACCCACCATTGGAAGGCCACGTTCGGCAGGGTGTCTTTTCTTCCCTCAACCACATTGTG GAGAAACGAGTCTTGGCACACTTGGCTCCCCTGTTTGACAACCCTAAATTGGATAAAGAGCTTCGGTCCA TGCTTCGGGAGAAGTTTCCTGAGTTCTGCAGCTCGCCCTCCCCTCCTGTGGAAGTCAAAATTGAAGAGCC TGTTTCCATGGAAATGGACAACCACCTCTCAGATAAGGATGAGAGTTGCTATGACAATGCAGAGGCAGCC TTCAGTGATGATGAGGAGGATCTTAATAGCAAAGGGAAGAAGAGAGAGTTTCGCTTCCACCCCATCAAGG AGACTGTCGTGGAGGAGCCGGTTGATGTCACCCCTTACCTTGATCAGTTGGACGAGTCCCTGAGGGACAA AGTGCTACAGCTACAGAAGGGAAGTGATACAGAGGCCCAGTGTGAGGTTATGCAGGAAATTGTGGATCAG GTCCTGGAGGAAGACTTTGACTCGGAGCAACTGTCTGTCCTGGCTTCCTGCCTACAGGAGCTCTTCAAGG CCCACTTTCGAGGGGAGGTGTTGCCTGAAGAGGTCACTGAAGAGTCCCTGGAGGAGTCAGTAGGGAAACC ACTGTACCTAATATTTAGGAACCTGTGCCAGATGCAGGAGGACAACAGCAGCTTCTCGCTGCTTCTGGAC CTTCTCTCCGAGCTGTATCAAAAGCAACCCAAGATCGGCTACCACCTGCTCTACTACTTGAGGGCAAGCA AAGCCGCCGCAGGGAAGATGAACCTGTATGAGTCGTTTGCCCAGGCTACCCAGCTGGGTGATCTGCACAC CTGTCTAATGATGGACATGAAGGCCTGCCAAGAGGATGATGTGCGACTGCTATGCCACCTCACACCTTCC ATCTACACAGAGTTTCCAGATGAAACTTTGAGGAGTGGGGAGCTGCTGAACATGATTGTGGCTGTTATTG ACTCTGCACAGCTCCAGGAGCTGGTCTGCCATGTGATGATGGGGAACCTGGTCATGTTTCGGAAGGACTC AGTCCTCAACATACTCATTCAGAGCCTAGACTGGGAAACGTTTGAGCAGTACTGTGCCTGGCAGCTCTTC CTGGCCCACAACATCCCCTTGGAGACCATTATTCCTATTCTCCAGCACCTCAAATACAAGGAGCACCCAG AGGCCCTGTCCTGTCTCCTGCTTCAGCTACGGAGAGAGAAGCCGAGTGAGGAGATGGTGAAGATGGTGCT GAGCAGGCCCTGCCATCCTGATGACCAGTTCACCACCAGCATCCTGAGGCACTGGTGCATGAAGCACGAC GAGCTGCTGGCAGAGCACATCAAGGCGCTGCTCATCAAGAACAACAGCCTGCCCCGCAAGAGGCAGAGCC TAAGAAGCTCTAGCAGCAAGCTGGCCCAGCTGACCCTGGAACAGATCCTGGAACACTTGGACAACCTAAG GCTCAACCTAGCCAACACCAAGCAGAACTTCTTTAGCCAGACACCAATCCTCCATGCGCTGCAGCATGTC CAGGCAAGCTGTGACGAAGCTCACAAGATGAAGTTCAGTGACCTCTTCTCCCTGGCGGAGGAATATGAGG ACTCTTCCACTAAGCCACCCAAGAGCCGGCGGAAGGCAGCTCTGTCCAGCCCTCGAAGTCGAAAGAATGC TACCCAGCCCCCCAATGCTGAAGAGGAGTCAGGCTCCAGCAGTGCCTCGGAGGAAGAAGATACAAAGCCT AAGCCTACCAAACGAAAACGAAAAGGCTCCTCTGCTGTGGGTTCTGACAGTGACTGAGGCCTGGATCCCC ACCCTGCCCCAGCCTGAGAAAGGTCCATGCAGCCCCACCTCTCCTCTCCTCCCAGGGCCTCCAGCTCTTT TCACCCCCTCTTCACTGCTGCTCCCACTGGTAGGTTCTCACAGCAACCAGAAGCTGTGCAGAAGCAGATC ATGGTGGAAGTCCTTTGCCGCCGGCTCCTCCCCAAGTCTCCGAAGAGCCATTTGGACAGAGAAAGGAGTG ATGAAGAGGCTGCTGGGCAGAGGAGCTGGGGTGACAGCAGATGCTGTGTGAGTGGGCATTTCCCCCTGCT TCCTGTCAGTCTGCATTTGTAGTCTGTGGACGGTGGTCATTACACCAGTGTGAAGTGGGCATCTGACCTG GTGTGGGCGGTGCCCATCTGGGCAGATGCCACAGCCTTCAGAAAAGAACCCAATGGCAGCGTGAGAGGCT CTTGCTGCACCTGGGATGCTTGGGTTGGCCCGGCATTGGCACTTCAAGCTGAGCCAGAGCCCAGGGGAGT CCTGGAGGCAAGTTCCTCAGAACTGTCAGATGGCCTGATTTTCTGTGGTTCTGTTTTTGCTTTTTCTTTT GGCAAAGATAATCGAGTTTTAAATTGTTTTTAAGTGATAATGAAACAAGCCAGAACATCTTTTGTGCCTG AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827687 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827687 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006847 same experiment
	EMAGE:31418 same embryo
	EMAGE:32001 same embryo
	EMAGE:31178 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source