EMAGE mouse gene expression spatial database

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EMAGE:32001

A830091I15Rik ( MGI:2136419)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006910_01	euxassay_006910_02	euxassay_006910_03	euxassay_006910_04	euxassay_006910_05

euxassay_006910_06	euxassay_006910_07	euxassay_006910_08	euxassay_006910_09	euxassay_006910_10

euxassay_006910_11	euxassay_006910_12	euxassay_006910_13	euxassay_006910_14	euxassay_006910_15

euxassay_006910_16	euxassay_006910_17	euxassay_006910_18	euxassay_006910_19	euxassay_006910_20

euxassay_006910_21	euxassay_006910_22	euxassay_006910_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

homogeneous

strong expression: see section 01 02 21 22 23

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 08 09 10 11 14 15 16

tongue

strong

spotted

strong expression: see section 11 14

spinal cord

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 05 06 17 18

trigeminal v nerve

strong

homogeneous

strong expression: see section 07 08 09 16

thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 15 16 17

facial vii ganglion

strong

homogeneous

strong expression: see section 02 03 04 05 18 19 20

vagus x ganglion

strong

homogeneous

strong expression: see section 06 07 17

trigeminal v ganglion

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 16 17 18 19 20 21

cervical ganglion

strong

regional

strong expression: see section 07 08 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9233
Entity Detected:	A830091I15Rik, ( MGI:2136419)
Sequence:	sense strand is shown >T9233 CGGGCACCGGCGTCTCTACCCTCCCGCGTCTGGTCCATGCTTCTCTCTCCCTTCATGCCCTTCCTAAGTC GCTGAGTCCCGGAGCTGCCCTCCTCCTTCTGCTTCTACACTTGTAGCCCAGCACCTTTACCGGATCCTGT TTGCTTTTTTTTTTTTTTTTTTTTCTTTCTCCTCCCAAGGCCGGGGTCTCTCTGCCAGGCCCCGCCCCTC TAGGCCTGGCATGTCCCCTGCTGCCCAGCGCCCATGGTCCTGACGCTGCTCTTCTCCGCCTACAAACTGT GCCGCTTCTTCACCATGTCAGGCCCACGGCCGGGCGCCGATCGGCTGACAGTGCCCGGACCGGATCGGAG TGGTGGCGCCAGCCCATGGTGGGCTGCGGGCGGTCGCGGGTCTCGCGAAGTGTCGCCCGGAGTGGGCACT GAGGTGCAAGGCGCCCTGGAGCGTTCGCTGCCTGAGCTGCAGCAGGCGCTGTCCGAGCTGAAACAGGCAA GCGCGGCGCGGGCTGTGGGCGCGGGTCTCGCCGAGGTCTTCCAGCTGGTAGAGGAAGCCTGGCTGCTGCC GGCCGTGGGCCGCGAGGTGGCCCAAGGTCTATGCGATGCTATACGTCTGGACGGTGGCCTCGACTTGCTG TTGCGGCTGCTTCAGGCACCGGAGCTAGAGACCCGTGTGCAGGCCGCGCGCTTGCTGGAGCAGATCCTGG TGGCTGAGAACCGGGACCGCGTGGCGCGCATCGGTCTAGGCGTGATCTTGAACCTGGCGAAGGAGCGCGA GCCTGTGGAACTGGCACGAAGCGTGGCGGGCATCTTGGAGCACATGTTCAAGCACTCGGAGGAGACGTGC CAGCGGCTGGTGGCGGCCGGAGGCCTCGACGCGGTGCTGTACTGGTGCCGCCGCACAGACCCGGCGCTGC TGCGCCACTGCGCTCTTGCGCTGGCGAACTGCGCGCTGCACGGGGGCCAGACGGTGCAGCGGTGCATGGT GGAGAAGCGCGCCGCCGAGTGGCTCTTCCCGCTCGCTTTCTCCAAGGAGGACGAGCTGCTGCGGCTGCAC GCCTGCCTGGCGGTGGCGGTGTTGGCTACCAACAAGGAGGTGGAACGCGAGGTCGAGCATTCTGGCACAT TGGCGCTTGTCGAGCCGCTCGTGGCATCGCTGGACCCCGGCCGCTTCGCCCGCTGCCTGGTGGATGCCAG TGACACAAGCCAGGGTCGTGGACCAGACGACCTGCAGAGCCTGGTGCTGTTGCTCGATTCGTCGCGTTTG GAGGCTCAGTGCATAGGAGCATTCTACCTGTGCGCAGAGGCTGCCATCAAGAGCCTACAGGGAAAGACCA AGGTGTTCAGCGACATCGGCGCTATCCAGAGCCTGAAACGCCTGGTTTCTTACTCTACGAATGGCACCAC GTCGGCGCTGGCCAAGCGCGCGCTGCGCCTATTGGGCGAGGAGGTGCCAAGGCGCATCCTGCCCTGCGTG GCCAGCTGGAAGGAAGCTGAGGTCCAGACCTGGCTACAGCAGATCGGCTTCTCCCAGTACTGCGAGAACT TTCGGGAGCAGCAGGTAGATGGTGACCTGCTTCTAAGACTCACAGATGAAGAACTCCAGACAGACCTAGG CATGAAATCAAGCATCACCCGCAAGAGGTTCTTTAGGGAGCTCACAGAGCTCAAGACCTTCGCCAGCTAC GCTACTTGCGACCGCAGCAACCTAGCGGACTGGCTGGGCAGCCTGGATCCTCGCTTCCGCCAGTACACCT ATGGCCTGGTCAGCTGCGGTCTGGACCGCTCCCTGCTGCACCGCGTGTCAGAGCAGCAGCTCCTGGAGGA CTGTGGCATCCGCCTGGGAGTGCACCGCACGCGCATCCTCTCTGCAGCCAGAGAAATGCTACATTCCCCG CTGCCCTGTACTGGAGGCAAGCTCAGTGGGGACACCCCAGATGTCTTTATCAGTTACCGGAGGAACTCAG GGTCCCAGCTGGCCAGCCTCCTGAAGGTGCACCTGCAGCTTCACGGCTTCAGCGTCTTCATCGACGTGGA GAAGCTGGAAGCCGGCAAATTCGAGGACAAGCTTATCCAAAGCGTCATAGCGGCTCGCAATTTTGTCCTG GTGCTGTCTGCTGGGGCGCTGGATAAGTGCATGCAGGACCATGACTGCAAGGACTGGGTGCACAAGGAGA TTGTGACTGCTTTAAGCTGTGGCAAGAACATTGTGCCCATCATTGATGGCTTTGAGTGGCCTGAGCCTCA GGCGCTGCCTGAGGATATGCAGGCTGTACTCACCTTCAACGGCATCAAATGGTCCCATGAGTACCAGGAG GCCACCATCGAGAAGATCATCCGCTTCCTACAGGGCCGCCCCTCTCAGGACTCCTCTGCCGGATCGGATA CCAGTTTGGAGGGAGCTACGCCAATGGGTCTGCCTTAACCTGTCCCCAGTTCCCGTGCCCTACTGTGACT CCTGATTTAGTTCCCTGCCCTTAAAGGAATAGCTCCTCTAGATGCCACCCTGACTGAGGCAAACCAGACT GTTCTCAGGCAATGGCTTTCCCGGCTGCCACTCTGTGGCCCACTTCTAATCCAGAGATGCCAGGCACAGG TTAGATGAGAGGACACTCCTGTGTCAGGCCCTGCCATCAGGACCCAGCTCCCATGCAGAACCATGTCCCT GCTTAGTTCTGGACATCCAAGACAGGGGAAGAACATTTCCCCATCTCCTGACCTGAGAGCAGCCAGCTTG AGTAAGGTGAGGTTGCCAGCCTCCCATAAGGGTCAATACAACGCCTGGATCCCCAAACCTGTGCTCAGGA GGAAGCTGGGGCTGAGAATCCCCCAAGGCTATCTACTCACATTGTGCCTGGACCCGGGCTCACCTGGCTT CCTCCTGCCTCATGGCCTTGCTCTGTAACTACCTGGTACCCCTTCTAGACTTCCTTTAGGCAGCCTCCTT TGGCATATTGGGAGTCAGGGTGTGGCTGAACTGGACCTCTGCCTGGCCTTGTTGCTCCTCCTGGTGCAAG AAGGGGTGCTTTGCTTATGCAAGGAGACCATAAGAGCTCACTCTAGCCCAAACCTTTGTGCGTGCCTAAG GCTCTTACCTAGCCTGCTCCATCTGACTTCCCCAGCTCCTCACCCACACAGAGCTTGTCACCCACCTGCT CAGGGAAGCCCCAACACCACCAGTAGCCCGAGCAGAACCTGCACCATGGGAAAAAGGCAAGCCATCCACA GGCAAGGGCTAATTCCTGGGGCAGTTTCTCCCACCGAAGTGTGTCAAATCCATTGGGAATCTCATCAAAA TTCAGAGCAGGCCACCTGGGAAGCACCTGGGCCTCTGCATTTTTCAGATTCCCAGGAGAGCTGATGAAAG AGAGCCCTCACTAATCCAGCCATTCCAAACACCCCCACCCTGGTCCCTGATCATCACAACAGCCTTTTCC TCTCTCCAGTTTTACAGAGCTGAGTGGCTGAGTGATGGGATCAGGTCCCCAAGTTATTGGTGGCAGCCTC AGGATCTTGCCTTAGTCCTGGTACCCAGATCTGTTCTCCTGAGACTCATCCAAGAGGCTGTGGCCCATGC TTGGTAGCTGCCTCTCCCTGGCCACAAAGAACTGAGCAGTGCAGGGTGGGTCTTGACTAAAAGGTGGAGC TAAGGGCATATAGAATGTCCCATGTCCCTCCCTCCACTGGACAAGAAAGGCAAGAAAGCTGGCTCTTGCC ATTACCCAGGCTCAAGCTCAGAGAGCTGGCACCACCCTGCTGTCGTCTTCTGGAGGCCATGGGGTCACAC CTCTCCTGAGGCTAGCCAGGTTACTTGCCCACGGCCCACATTATCAGAGAGCCAAAGGGCTTTCTCTCCA GGCCTGGGGACACTTTCCTCCTACCGTCCATCTTCCATGGGTCATGGCTGGCCTCTGGCTGTGACTATTT GCAGTTTTGTCACCAGGAACAACCCCCGCATCCTATTCTGAGGTGAGGCAGATGGAATGGTATCCTTGAT GACAAATTCAATGACAATACAGTGTATGAGTTCACCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6834056 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834056 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006910 same experiment
	EMAGE:31418 same embryo
	EMAGE:31178 same embryo
	EMAGE:31448 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source