EMAGE mouse gene expression spatial database

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EMAGE:31348

Cstf2t ( MGI:1932622)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002070_01	euxassay_002070_02	euxassay_002070_03	euxassay_002070_04	euxassay_002070_05

euxassay_002070_06	euxassay_002070_07	euxassay_002070_08	euxassay_002070_09	euxassay_002070_10

euxassay_002070_11	euxassay_002070_12	euxassay_002070_13	euxassay_002070_14	euxassay_002070_15

euxassay_002070_16	euxassay_002070_17	euxassay_002070_18	euxassay_002070_19	euxassay_002070_20

euxassay_002070_21	euxassay_002070_22	euxassay_002070_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5464
Entity Detected:	Cstf2t, ( MGI:1932622)
Sequence:	sense strand is shown >T5464 AATTCAATCTCCGCTGTCCTCTCGGCAGCAATCATGTCGAGTTTGGCGGTTAGAGACCCAGCCATGGATC GATCGCTGCGTTCGGTGTTCGTGGGGAACATTCCGTATGAGGCGACGGAGGAGCAGTTAAAGGACATTTT CTCAGAGGTTGGTTCAGTTGTCAGTTTCCGTCTCGTCTACGATAGAGAGACTGGGAAGCCCAAGGGTTAT GGCTTCTGCGAGTACCAAGACCAGGAGACTGCGCTCAGTGCCATGCGAAACCTCAATGGGCGAGAGTTTA GTGGGAGAGCGCTTCGGGTGGACAATGCCGCCAGCGAAAAGAACAAGGAGGAGTTAAAGAGCTTAGGCCC GGCGGCCCCCATCATTGACTCCCCCTATGGGGACCCTATCGATCCAGAAGATGCTCCAGAATCGATTACT AGAGCAGTCGCCAGCTTGCCCCCAGAGCAGATGTTTGAGCTCATGAAGCAGATGAAGTTGTGTGTCCAGA ACAGTCACCAGGAAGCTCGAAACATGCTACTTCAGAACCCACAGTTGGCGTATGCTTTGCTGCAGGCACA AGTGGTGATGAGAATCATGGATCCAGAGATTGCACTGAAAATTTTGCATCGTAAGATACATGTCACACCA CTGATCCCAGGCAAATCTCAGCCGGTCTCTGGGCCTGGCCCTGGTGGGCCTGGGCCTAGTGGGCCTGGCG GCCCTGGCCCCGGCCCTGCCCCTGGCCTCTGCCCGGGACCTAACGTCATGTTGAACCAACAGAATCCTCC TGCCCCTCAGCCTCAGCATCTGCCAAGAAGACCTGTGAAGGACATTCCACCTCTGATGCAGACCTCTATC CAGGGAGGAATTCCAGCTCCAGGGCCAATACCAGCTGCAGTTCCTGGACCTGGACCTGGTTCCTTAACTC CAGGAGGAGCAATGCAGCCACAAGTTGGCATGCCAGTGGTTGGTCCAGTGCCCCTGGAGCGAGGACAGAT GCAGATATCAGATCCTAGACCTCCGATGCCTCGTGGACCCATGCCTTCTGGTGGCATACCTCCTCGAGGA CTACTGGGAGATGCTCCAAATGACCCACGTGGAGGGACTTTGCTCTCAGTGACTGGAGAAGTAGAGCCCA GGGGCTATATGGGACCACCCCATCAGGGTCCTCCAATGCATCATGGTCATGACAACCGTGGCCCTGCCTC ACATGATATGAGAGGAGGACCATTGGCAGCAGATCCCAGAATGCTAATTGGAGAGCCCAGAGGTCCCATG ATAGATCAGAGAGGTCTACCTATGGATGGTAGAGGAGGTAGAGAATCTCGAGGGATGGAGACTCGGCCCA TGGAAACTGAGGTCTTGGAGCCACGAGGAATGGAGAGAAGGATGGAGACCTGCGCGATGGAAACCAGAGG GATGGAAGCAAGAGGCATGGATGCAAGAGGACTAGAGATGAGGGGCCCTGGCCCTAGTTCCAGAGGTCCG ATGACTGGTGGAATCCAGGGTCCTGGCCCTATTAATATGGGGGCAGGTGGCCCTCAGGGACCTAGACAGG TTCCAAATATTGCAGGAGTGGGAAATCCTGGAGGTACCATGCAGGGGGCAGGTATACAAGGAGGAGGGAT GCAGGGAGCAGGTATGCAAGGAGGAGGGATGCAAGGAGCAGGCATGCAAGGAGGAGGGATGCAGGGAGCT GGCATGCAAGCAGGGATGCAGGGAGCTAGCATGCAAGGAGGGATGCAGGGAGCTGGCATGCAAGGAGCCA GTAAGCAAGGTGGAGGCCAGCCTAGCAGTTTTAGCCCTGGGCAAAGCCAGGTAACTCCACAAGATCAAGA AAAAGCAGCTTTGATCATGCAGGTTCTCCAGCTGACTGCGGATCAGATTGCCATGCTGCCTCCCGAACAA AGGCAGAGCATTTTGATTTTAAAGGAACAAATCCAGAAATCCACTGGGGCTTCTTGAATGGTTTTCAACT AAGAAGCACTTAGTTACTCCTTCAGAGTTTATTCTGTGGCATGAAGTGGTGCAAAAAGCTGTCTTCTGTG TTCGGACACTTTAACCATTTAAGGTTTCCTTCTCCCTAGATCTTAATCTTTCTTCTAGTCCTGTCACTTT CTGCTTCCCTTTTAGCTTTTTGATGGAGGTTATGGAGTGGAAGGAGTGGGCCTGTTCACTTTGTCACTGT TACTCTACCACGTACCCTGAAAATAACTACATCATCCACCAAGTAAGGCTATGAGGAAGATGCAGGAGGA ATAACATGTCTGCACTTTGTTAACTGCGTCTTTAAAAATCCCGAGTAAGCTGGGAACTACTTAAAAATTG AAAGTGACTTGTTACAGTATTGATATACTAAGATGGTTTAAAGGTTTTTGGTTGTATATTAACTGAATGT CAGCATCTTAAGATACACTTTTTGGTAAACCAAAATACTGTAGAGTAATAAGATTAATGTTTAGTTATTT TGGAATTATTTTGAAATATTGGAGCTAACAGTCTGTGGTGTAGATGTAGGGTTTTTTTTTGTTTTTGTTT TTTTTTTTAAGCATTGTTATCTGTAAAAAGGTAATTTTCATTTACCTGACTTTTTTGAGACAACTAATAT TCTTGCCTGGTCCCACCTGGTGATTTTGCAGAATAGTTGTAGTGTCAGCTGAATTATATAAAGCCGCCTC TGAGGAGACTCAAGTGATTTCCTAATACATTTCTCTAAAAAAATTCTTAAGCACCAAGTCTGGTTGTAAG TAGTTTTTTCATGTCATCTGAAAATAGCAGTTAGACATGGGGTCGGACTTTCTTGAGTAATGGAGGGTTT TTCAGTAAAGCTCCCACCCAGGTTCTTGATAAACCACTATCCATATGCAGATGGAATCCATTTGGTCAGC AGGAATCAGAAGTTAAAAAATCTTAGTCTTCGAATTTTGACGTGTCTTACAGTTTGATAACTTTCACAAA GTACTTTCCTGCCATCAGCTTAACTAGAACTGAGGCCCAAGTGATCTGACAGCTCTGCTCAACTTAGTAT TTTATTTCTTAGAACTCTCAAGAGCCATTTGGTCATAACATACATTCCTATCAGATGTGTTTTAAAATAA GGAGTGTGGAATTTAATACATTTCCTTTAGAGCTACCATACTATTTTTTGACATTAAGTGTGTGGCACCT AGACACCATGTCATATCTAGTTAATGAGCAGAAACAAGCACAAGTTCCCACTTGACCAAGTGATAGTCCT CTGTAGGAAACTACCCAGCTACAGAGGGAATAGTAGCCTTAGGGAGAGAGCTGACCCAAGGGTCTACTTT GTCCTTGGAAAGTTTGAGCATTTTCAGTGTACAGAGTTTTCATTCCTAGGCTATTTTCCATCGACTTAGT TTTTTGTGCTAGTGTTAAACTCTCTGTGGTTTCCTCTGCTTCTCCTTTGCTGAAGTTGGTTTGTGTTTTG TACTTTTGTGCTAGTTCTGGCTAATTCCAATTGCTTGCTTTCGAAATTGCGGTTGCTAGCCCAAAACTTC TTATAGTCTTTGTTATAAGAAAATCTCTGCATTGTTTAATGAAAATTAAATAAAAAGGTTGTATAATTAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5373155 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5373155 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002070 same experiment
	EMAGE:29480 same embryo
	EMAGE:31829 same embryo
	EMAGE:31350 same embryo
	EMAGE:31357 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source