EMAGE mouse gene expression spatial database

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EMAGE:31457

Ndrg1 N-myc downstream regulated gene 1 ( MGI:1341799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004423_01	euxassay_004423_02	euxassay_004423_03	euxassay_004423_04	euxassay_004423_05

euxassay_004423_06	euxassay_004423_07	euxassay_004423_08	euxassay_004423_09	euxassay_004423_10

euxassay_004423_11	euxassay_004423_12	euxassay_004423_13	euxassay_004423_14	euxassay_004423_15

euxassay_004423_16	euxassay_004423_17	euxassay_004423_18	euxassay_004423_19	euxassay_004423_20

euxassay_004423_21	euxassay_004423_22	euxassay_004423_23	euxassay_004423_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

mandible

moderate

regional

moderate expression: see section 09 10 11 17 18 19 20

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

palatal shelf

strong

regional

strong expression: see section 13 14

diencephalon meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

rectum

strong

regional

strong expression: see section 13

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

anterior naris

strong

regional

strong expression: see section 13 moderate expression: see section 14 15 16

stomach pyloric region

strong

regional

strong expression: see section 06 07 08 09 10 11 12

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

hindbrain meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

dorsal root ganglion

strong

regional

strong expression: see section 08 moderate expression: see section 07 09 10 11 15 16 17 18 19

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

external naris

moderate

regional

moderate expression: see section 12

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 12 16 17 moderate expression: see section 10 11 18 19

naris

moderate

regional

moderate expression: see section 15

upper jaw

strong

regional

strong expression: see section 11 17 moderate expression: see section 09 10 18

nasal septum

strong

regional

strong expression: see section 13 14 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7576
Entity Detected:	Ndrg1, N-myc downstream regulated gene 1 ( MGI:1341799)
Sequence:	sense strand is shown >T7576 CCCACGCGTCCGCCCACGCGTCCGAGGCTACCCTCTCGCGTTGCAGGCAGATTGCTCACCCATCTTCGTC TCCCTCTTCACCTCCCGCGGGGCGCTGCACCTCCAACCTTGTGGTTGGCTCCTTTCCGTGCTTGCTTGCT CATTAGGTGTGTGATAGCACGGAAATGTCCCGAGAGCTACATGACGTGGACCTCGCTGAGGTGAAGCCTC TGGTGGAAAAAGGGGAGAGCATCACTGGTCTCCTGCAAGAGTTCGATGTTCAGGAGCAGGATATTGAGAC CCTACATGGCTCCCTGCATGTCACCCTGTGTGGGACCCCCAAGGGCAACCGTCCTGTCATCCTCACGTAT CACGACATCGGCATGAACCACAAGACCTGCTACAACCCCCTCTTCAACTCTGAGGACATGCAGGAGATCA CACAACATTTTGCTGTCTGCCATGTGGATGCCCCTGGCCAACAGGATGGTGCCCCTTCCTTCCCAGTGGG GTACATGTACCCCTCAATGGATCAGTTGGCTGAAATGCTTCCTGGAGTCCTTCACCAGTTTGGGCTCAAG AGTGTCATTGGCATGGGGACAGGAGCTGGCGCCTACATCCTGACCCGCTTCGCACTCAACAACCCTGAGA TGGTAGAGGGTCTCGTGCTCATGAATGTGAACCCCTGTGCTGAAGGCTGGATGGACTGGGCTGCCTCTAA GATCTCAGGATGGACCCAAGCCCTGCCTGACATGGTGGTGTCCCACCTCTTCGGCAAGGAGGAGATACAC AACAACGTGGAGGTAGTGCACACGTACCGCCAGCACATCCTCAACGACATGAACCCGAGCAACCTACACT TATTCATCAGCGCCTACAACAGCCGGAGAGACCTGGAGATTGAGCGGCCAATGCCTGGAACCCACACTGT CACTTTGCAGTGCCCTGCTCTGCTGGTAGTTGGAGACAACTCTCCTGCCGTGGATGCTGTGGTGGAATGC AATTCTAAATTGGACCCAACGAAGACCACCCTGCTCAAGATGGCAGACTGTGGAGGCCTTCCTCAGATCT CTCAGCCCGCCAAGCTTGCTGAGGCCTTCAAGTACTTTGTGCAGGGCATGGGATACATGCCTTCTGCCAG CATGACTCGCCTGATGCGGTCCCGCACAGCCTCTGGCTCCAGTGTCACATCCCTGGAGGGCACCCGTAGC CGCTCCCATACCAGCGAGGGCCCCCGAAGCCGCTCGCACACCAGTGAGGGGTCCCGTAGCCGCTCCCATA CCAGTGAGGATGCCCGTCTCAACATCACCCCCAACTCGGGTGCCACCGGAAACAATGCCGGGCCCAAGTC CATGGAGGTGTCCTGCTAAGCAATCTGTGCATCAGTCACCCTGGACTGGTGGCCTCTCCCTTAGCCCCCA ACCCTTGCCTGCCACCCTACGCTAATGCGGTATTAATCTAAAGCTGCTTTTGTAAGAATGAACTCTGGCG GTGGCAGACCCAGGCTGTTCTGACTGCCTCCTGGGACCAGGGTGGGTGGTGGTGGACCAAAGGCAAGAAG CAGTTCAGCGTACTGTTCTGCTAACAAGTGGCCTGGTGGACCCTCTCTCTACCCTCAGACACCAAACTGC CAAAACTAAGAAATAGATAGCTGTAACACTGCCTGGCTCCAAGCCCAGGCTGATCCAGCATTCTGGTTCA TAGGCGTTTTGCCAACCTGCCTACTTCCTGTTTCCTCCCCACTAAACTCATTCCTGGAAACAAACTTCTG TTTTGAAAGGAGAGAGAGAGGCAGGAAAGTTGGGCGCCTTATTTTAAGTAGAGAAACCAAGAAAGGAAGA GAAAAAAAGGCAGGGTCTAGGGCAGGTAGAGGAAAAACACAACAAGTCTACTAGGCTCACTTGAAGGAAT TGATTTTAAAGTTCTGGACACTGAAACCCACAACCTGTTTTTCTGGCAACCTTTTAACGTGCAAGAAAAA CTGGTAACAAACCCCAGGCTTTATAGAGAAAGTGATCCAGCAAATGTGGGGCATCAGTGATTTAAGACTC TTGCTATGCAGTCTACTGTACCCCGCTCCCATTCCACAGTGAATGAGTGGATGACACCTCTGTCCCTCGG GAAGATGGTTTTGATTTGTTGTTGCATTCTTTGAGGCAGAGGGAGAACCCAGCAGGTGACAGGGGTCCTT GTCTGGGATGAGTCTTTAGGATGGGAAGGGTGGGGATAGAATGGCCCTGGGATTTTGAGGACTCATGGTA GGGCCAAGTGCTAGAACCTGGTGGGTGTGATTTCATTGATCTGGAATCTTGTGTGACTGAGTCACACCTT GTCTCCCATAATTGATGTCTGGCATTGATTTGAAACTTGATAGCTGAATGTCTCTTAACTGGCCCATCGC TGGTTCTTCAGAGAGAACCGTATGTTCTAGTGGGGGAAGGACTGGTTTTGGGTTCTGTACATTCCACGGG AACTTGCTTCCCTCTCCTGCCCCTCCTGCTGACTGGGTTTGTAGCATTGGCCTCTTAAGCAATGCCAGCC TAATGCCCATGCTCCATACTACTGCCTTTTGATTCTTACTGTGGCTAACCTTGAACCTTTCCTATAGGAA ATGTGATCAGATCAGAGACACTTGATCGTTGGGAGTCAGCCCTCAAGGCCTGGCGACTTCCAGGATCTTG GAGTTGCTAGAGGAAAAGTAGTTATGCCCTATAGGCGGCATGAGAGACAAGGGATCAGTTTACCTGCCAA CTCCTACCGTGGAGGAGGAGTTCCACTGACCTTGTGTAGAAAGATCAGGAATCCAGGCCAGGGTGATTGA ATGCTGACAAGAAATCACTGTATATGTCTCTCCAGGGCAGTTTCTTAATAAGATGTTTGTATTTATTTCC AGACCAATAAATTTGTAACTTTGCAGTGAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242075 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242075 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004423 same experiment
	EMAGE:31417 same embryo
	EMAGE:30372 same embryo
	EMAGE:30371 same embryo
	EMAGE:31481 same embryo
	EMAGE:31403 same embryo
	EMAGE:31402 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source