EMAGE mouse gene expression spatial database

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EMAGE:31671

D19Wsu162e ( MGI:107577)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_007077_11	euxassay_007077_12	euxassay_007077_13	euxassay_007077_14	euxassay_007077_15

euxassay_007077_16	euxassay_007077_17	euxassay_007077_18	euxassay_007077_19	euxassay_007077_20

euxassay_007077_21	euxassay_007077_22	euxassay_007077_23	euxassay_007077_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

adrenal cortex

moderate

regional

moderate expression: see section 06 07 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7219
Entity Detected:	D19Wsu162e, ( MGI:107577)
Sequence:	sense strand is shown >T7219 GAAGGGAAGGAGGAAGCAGAGGGAAGAAGAGAGGGAGGAGGAGGGGTGGCGGCGCCGTGGCGGAGGAGCA GGAGCAGGAGGGGGATGGAGAGGAGAAGGCTCCTGGGTGGCATGGCGCTCCTGCTCCTCCAGGCGCTGCC CAGCCCCCTGTCAGTGAGGGCTGAGCCCCCGCAGGATAAGGAAGCCTGTGTGGGTACCAACAATCAAAGC TACATCTGTGACACAGGACACTGCTGTGGACAGTCTCAGTGCTGCAACTACTACTATGAACTCTGGTGGT TCTGGCTGGTGTGGACCGTCGTCATCATTCTGAGCTGCTGCTGTGTCTGCCACCACCGCCGAGCCAAGCA CCGCCTTCAGGCTCAGCAGCGGCAACATGAAATCAACCTGATCGCTTACCGGGAAGCCCACAACTACTCA GCGTTGCCGTTTTACTTCAGGTTTTTGCCAAACTCTTTGCTACCTCCTTATGAGGAAGTGGTGAACCGAC CTCCAACTCCTCCCCCACCGTACAGTGCCTTCCAGCTCCAACAGCAGCAGCAGCTGCTGCCACCTCCTCC TCAGGGTGGCCCTCCAGGTGGCAGCCCCCCAGGCACTGACCCGCCGCCCCAGGGCTCCCAGGGAGCGCAG AGCAGTCCTTTGTCCGGACCGAGCCGAAGCAGCACAAGACCTCCAAGTGTCGCAGATCCTCAGTCCCCTG AAGTGCCCACTGACCGGGAAGCCACCAAAGCCTCTGGGACGGAGTCTGGTAGCCCCATGGCCGGCCACGG GGAGCTGGACCCGGGCGCCTTCCTGGACCAGGATTCCGAGTGCAAGGAGGAGCTACTGAAAGATTCGCGT TCTGAGCGTGGCGGCGTGTCCCCTGACAGTGAAGACAAGACCCCCGGCAGGCATCGCCGCTTCACAGGTG ACTCAGGCATTGAGGTGTGTGTGTGCAACCGGGGCCACCATGACGATGACCTCAAAGAGTTTAACACGCT CATAGATGATGCTCTGGACGGGCCCCTGGACTTCTGTGACAGCTGTCATGTACGGCCTCCTGTTGATGAA GAGGAAGGTCTCTGCCTGTCCTCGGAGGGTCAGGCTCGGGAGCATGGGCACCCCCACCTGCCACGGCCGC CTGCGTGTCTGCTGCTAAACACCATCAATGAGCAGGACTCTCCAAATTCCCAGCACAGTGGCTCCCCGAG CTAGGACACGCCCTGCCTGCACCTAGGAGCTCGACGACAACCAGGGTCAGGGACACCCACGAGAGAAGCA TTAAGTAACATGGACATGTGGCCCGGCCACTTTGTCCTCTTATATTTTTTCCCCCTCCTATCCCACCTTG CCCACATCTCCAGGTACAAATTCAGGGTGTGGATGCCTCCTCTCCCCACAAAAGCACAGTGTTCTAGAAG ACGGAGAAGCTGCTTCTGTGACTCCCGCACCCCCCTTTCAGAGCTGTGTCCCACCTACCTGCCTGGCTGA GAAACACAAGTTTGCAAAGCCCCCCCCCGAGGACTGGGGCTGGACTGTGCCCTAAGGTGGTGCTCAGCGG TGACAGAGTGGATTCACGAGAGAACCGTGTCTCAGTCTAGGAGACACCACTTCTCAGAGAGAAGAAGAAA ACCCTCAGAGGCCAGTGGATTGTCACTGTGGCTGCCAGTGGCTGCAGAGCACTGGTCTAACCCTGTGGGA GGGTGACAGCTAGTCAGGGTGGTGAGGGACTTGCTGAGCTGACAAGGCCCAGGCAGAAAGTACACATCTG TGCGTTGTAGAGGGGACCCTTCCCAGCCTGGTTCAGGTGTAGATGATACTACGTTCTCTGGGTTTTCCTT TAAAAACAAGCAACAAAGGCTGGCAGGAAACAGCCAGACCAGTGGGCAGGCAGGGACTCTTCGTTACAAG GGAATTCTGCTGCTTCTCCCCAGGCTGAGTTCCCAAGAGTCTGACTACCATTGGGCCAGGAGGAGAGAGC CTGAACAGGCCCGGTGGTGGGAGCCTTGGGCCTTCTGTTGAGTGGAGACCCAAGTCTGAAGGCTGGGAGA GCTAAGAGCTTCCTTCAGTTCCGCCCTTTGTCCGTGCCCACAGGTGGTCAGTGGTCCTCGCCCCTCCTGA ACACAGCCTGCGGTCTCTGTCTCCCTGTCCTCTGTAAGCAGATGGACTGAGGAAGTTGTGTGTTCAGGGC AAGGTGGCCTCATAGCCTGGTTCTCACACAGTATTTCCTGTTGCGTTTGACTTAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:4160029 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4160029 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007077 same experiment
	EMAGE:31758 same embryo
	EMAGE:29947 same embryo
	EMAGE:31285 same embryo
	EMAGE:31707 same embryo
	EMAGE:31678 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source