EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31758

2410166I05Rik ( MGI:1924074)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007068_01	euxassay_007068_02	euxassay_007068_03	euxassay_007068_04	euxassay_007068_05

euxassay_007068_06	euxassay_007068_07	euxassay_007068_08	euxassay_007068_09	euxassay_007068_10

euxassay_007068_11	euxassay_007068_12	euxassay_007068_13	euxassay_007068_14	euxassay_007068_15

euxassay_007068_16	euxassay_007068_17	euxassay_007068_18	euxassay_007068_19	euxassay_007068_20

euxassay_007068_21	euxassay_007068_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7216
Entity Detected:	2410166I05Rik, ( MGI:1924074)
Sequence:	sense strand is shown >T7216 AGGGGCTGAGGAAAGGAAACCTCGTGCTCCTGGAAATGGCCTGATATGAAGGAGCCACCCCTCCAGCTTT CCCTGTGGGTGCCTCGTCCTTCAGGTACAGGAGCTGGCTGAAACATCAGGAATGGAAGCCAATGTGACTA TCCCAATCTGGCAAAACAAGCCACATGGGGCTGCTCGCAGTGTGGTAAGAAGAATTGGGACCAATCTCCC CCTGAAGCCCTGTCCACGGGCATCCTTTGAGACCCTGCCCAACATCTCTGACCTGTGTTTGAAAGATGTC CCTCCAGTCCCTACTCTGGCTGACATTGCCTGGATTGCTGCAGATGAAGAGGAAACATATGCTCGGGTCA GGAGTGATACACGCCCTCTGAGACATACCTGGAAGCCTAGCCCTCTGATTGTCATGCAACGTAATGCCTC AGTGCCCAACCTGCGTGGCTCTGAGGAGAGGCTCCTGGCCCTGAAGAAGCCGGCCCTGCCTGCCCTGAGC CGCACCACTGAGCTGCAGGATGAGCTCAGCCACCTGCGCAGCCAGATCGCTAAAATCGTGGCGGCAGACG CAGCTTCGGCTTCATTAACACCAGATTTCTTCTCTTCCGGAAGTTCGAATGTCTCTTCTCCTTTGCCTTG CTTTGGGTCCTCCCTCCACTCTACAACTTCCTTTGTCATTAGTGACATCACCGAGGAGACGGAGGTGGAG GTCCCTGAGCTCCCAACCGTCCCCCTACTTTGTTCTGCCAGCCCTGAATGTTGCAAACCAGAACACAAAA CTACCTGCAGCTCGTCTGAAGAGGATGACTGCATCTCTCTGTCCAAGGCCAGCAGCTTTGCAGACATGAT GGGCATCCTGAAGGATTTTCACCGGATAAAACAGAGCCAAGACCTGAGCCGGAGTTTACTGAAGGAAGAA GACCCTGCTGTCCTCATCTCTGAGGTCCTCAGGAGGAAGTTTGCCCTGAAGGAAGAAGACATCAGTAGAA AAGGGAACTGACAGCATTCACTGCAAACTGTCTCTTGCTCCTGAACTTCAATAGCTGCCTTCCTCACCGC AGATGTCTGTCTCCGAGATCTGCAACCGTCTTGCTGACTAGGGCTTGGGCTAAAAGAAAAGAGCCGGATT ACTCGCTGTTTCCTGCACTTAAGCCTTGGCAGGAGCCAAGGTCTATGGTTTTTTGAGATGAAGCATTTGT TACAGATAATTGGGTAGAGTGGAGTGTATCTGCTTAAGGGGTGAAGTTTGTTTTTTCTTGTCTCTGAAAA CTAAGCCCTCAATGAACGACTTAATTAACTCACTGCATTAGTCTCCATAATTGGTCTCGCTGGACACGTG CACAGCTGTGGCTCTCAGCAGCTTCCCTGCAGCGCCACTGGGCTAAGGGGGAGCGGCCGTTTATGTTCAT GTTCACAGGAAGGTCTTCTGCTGCTCCATCCTAGACCTGCCCCAGTGCCCAGCAGTCACCCTTCCCCTCT AGCTACAGAGTCCAGGCCACTGGACCAGCCAACATCTGGGCTGCTGCAGGGAGGCCTGGCCTATTTTTTC TTAAAACATATTTTATAAAACTACAACCTGATCTACCCTCCAGGGCCTCCTCAGTTCCACTGATTGAAAT TTTCTTCTTCCATGGACTAAGTCCATCTAGGAAGGAAAAATGAGGAAAAAGAGTGTGGTATCCTCCTTCT AAGACAGTGACCGATTTCTCTGGGTTGGGCCAAGACTTTATTTACTGCATCATGCATGACTCACTCAGGG GCCCTTGGGGCCCTCTTCTCTATGATATGAATACTGTGTGTGTGAGTTGAAGCATTACCTGATATTAAAT GAAGGATTTAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4159289 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4159289 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007068 same experiment
	EMAGE:29947 same embryo
	EMAGE:31671 same embryo
	EMAGE:31285 same embryo
	EMAGE:31707 same embryo
	EMAGE:31678 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source