EMAGE mouse gene expression spatial database

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EMAGE:31682

Chst5 ( MGI:1931825)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

foot

weak

regional

weak expression: see section 07 08 20 21

femur

moderate

regional

moderate expression: see section 08 17 18

hand

weak

regional

weak expression: see section 01 02

humerus

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 01 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T580
Entity Detected:	Chst5, ( MGI:1931825)
Sequence:	sense strand is shown >T580 TCTCAGNAGTTGGCTACTGGACACCGACTGAGCGGCTCTTTGTGTGCGCAGTGGGTGCGCAGCGCAGAAG CGCAGCGGGCAGCGCAGGCCNTAGCCAGAGGTATGCGGCTACCCCGTTTCTCCAGCACTGTCATGCTTTC GCTCCTGATGGTACAGACTGGCATCCTGGTCTTCCTGGTCTCCCGGCAAGTGCCATCGTCCCCAGCAGGC CTTGGGGAGCGTGTGCACGTGCTGGTACTGTCCTCGTGGCGCTCGGGCTCGTCCTTCGTGGGCCAGCTCT TCAGCCAACACCCCGATGTCTTCTACCTGATGGAGCCGGCTTGGCACGTCTGGGATACGTTGTCGCAGGG CAGTGCCCCCGCACTCCACATGGCCGTGCGTGACCTGATCCGCTCAGTGTTCCTATGCGACATGGACGTA TTTGATGCCTACCTGCCCTGGCGCCGCAACATCTCGGATCTCTTCCAGTGGGCGGTGAGCCGCGCATTGN GCTCACCTCCGGTCTGCGAAGCCTTCGCTCGTGGCAACATCAGCAGCGAGGAGGTGTGTAAGCCT
Notes:	The probe template was PCR amplified from IMAGE:1885262 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885262 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009256 same experiment
	EMAGE:31292 same embryo
	EMAGE:31614 same embryo
	EMAGE:29653 same embryo
	EMAGE:31545 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source