Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31734

Rdh5 retinol dehydrogenase 5 ( MGI:1201412)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31734 EMAGE:31734 EMAGE:31734 EMAGE:31734 EMAGE:31734
euxassay_009235_01 euxassay_009235_02 euxassay_009235_03 euxassay_009235_04 euxassay_009235_05
EMAGE:31734 EMAGE:31734 EMAGE:31734 EMAGE:31734 EMAGE:31734
euxassay_009235_06 euxassay_009235_07 euxassay_009235_08 euxassay_009235_09 euxassay_009235_10
EMAGE:31734 EMAGE:31734 EMAGE:31734 EMAGE:31734 EMAGE:31734
euxassay_009235_11 euxassay_009235_12 euxassay_009235_13 euxassay_009235_14 euxassay_009235_15
EMAGE:31734 EMAGE:31734 EMAGE:31734 EMAGE:31734 EMAGE:31734
euxassay_009235_16 euxassay_009235_17 euxassay_009235_18 euxassay_009235_19 euxassay_009235_20
EMAGE:31734 EMAGE:31734
euxassay_009235_21 euxassay_009235_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 12 14 15 16 17 18
4th ventricle
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1202
Entity Detected:Rdh5, retinol dehydrogenase 5 ( MGI:1201412)
Sequence:sense strand is shown

>T1202
CCTCGAGACTGTTGGCCTACTGGACACATTGTGTTGCCTGCCAGCTTTCCCCAGAGCCTAGGCTGCCCTC
AGCAGGGCATCTCATCCCATCATGTGGCTGCCTCTGCTTCTGGGTGCCTTGCTGTGGGCAGTGCTGTGGT
TGCTCAGAGACCGGCAGAGCCTGCCGGCCAGTGATGCTTTCATCTTCATCACTGGCTGTGACTCTGGCTT
TGGGCGCCTTCTGGCACTGCAACTTGACCAGAAGGGCTTCCAAGTCCTGGCCGGCTGCCTGACCCCCTCT
GGAGCAGAAGACCTGCAGCAGATGGCCTCCTCCCGCCTCCACACAACACTACTGGATATCACTGATCCCC
AGAATGTCCAGCAAGTTGCCAAGTGGGTGAAGACACGTGTTGGAGAAACTGGACTTTTTGGTCTGGTGAA
TAACGCTGGCGTAGCTGGTATCATCGGGCCCACACCATGGCTAACACAGGATGATTTCCAGAGAGTACTG
AGTGTGAACACACTGGGGCCCATCGGTGTCACCCTTGCCCTGCTGCCCCTGCTACAGCAGGCCAGGGGTC
GGGTGGTC
Notes:The probe template was PCR amplified from IMAGE:2182461 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2182461 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009235 same experiment
 EMAGE:29736 same embryo
 EMAGE:29681 same embryo
 EMAGE:30971 same embryo
 EMAGE:29679 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS