EMAGE mouse gene expression spatial database

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EMAGE:31763

Tk1 ( MGI:98763)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001974_01	euxassay_001974_02	euxassay_001974_03	euxassay_001974_04	euxassay_001974_05

euxassay_001974_06	euxassay_001974_07	euxassay_001974_08	euxassay_001974_09	euxassay_001974_10

euxassay_001974_11	euxassay_001974_12	euxassay_001974_13	euxassay_001974_14	euxassay_001974_15

euxassay_001974_16	euxassay_001974_17	euxassay_001974_18	euxassay_001974_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 06 07 08 09 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4559
Entity Detected:	Tk1, ( MGI:98763)
Sequence:	sense strand is shown >T4559 GGCGAGCAGTAAGTCGAAATTTTTCCACCCACGGACTCTCGGTGCTAACTAAGGTTTGCACAGCAGCCAT GAGCTACATCAATCTGCCCACCGTGCTGCCCAGCTCCCCCAGCAAGACTCGGGGGCAGATTCAGGTGATT CTCGGGCTCATGTTCTCAGGGAAAAGCACAGAGCTGATGAGAAGAGTCCGGCGCTTCCAGATCGCCCAGT ACAAGTGCCTGGTCATCAAGTATGCCAAAGACACGCGCTATAGCAACAGCTTCTCCACACATGATCGGAA CACCATGGACGCATTGCCAGCCTGCATGCTCCGCGATGTGACCCAGGAGTCCTTGGGTGTGGCCGTCATT GGCATCGATGAGGGGCAGTTTTTTCCTGACATTGTGGATTTCTGTGAAATGATGGCCAACGAGGGCAAGA CAGTAATTGTGGCAGCGCTGGATGGGACCTTCCAGAGGAAGGCTTTCGGCAGCATCTTGAACCTGGTGCC CCTGGCGGAGAGTGTGGTGAAGCTCACCGCTGTGTGCATGGAGTGCTTCCGAGAAGCTGCCTACACGAAG AGGCTGGGCCTGGAGAAAGAGGTGGAGGTGATTGGCGGAGCCGACAAGTATCACTCCGTGTGCCGCCTGT GCTACTTTAAGAAGTCTTCAGCCCAGACTGCTGGCTCAGACAACAAGAACTGTCTGGTGCTGGGGCAGCC GGGAGAGGCCTTGGTTGTCAGGAAGCTCTTTGCCTCTCAGCAAGTCCTACAATACAACTCTGCCAACTGA GGGGACCTGAGGCCTGCCAGCTCCTACCCAGGTTGGACTCTCAGAGAGCAGGGGGAGCGCGGGCCTGCCA TTCCTAATGGACAATGTACCTTGAACAGGCTGCCACTCGCTGAAGCCGTTTTCAGTTCCCTTCTTGATTG CCAAGATGCCTCAATGCAGACTGGAGCCCAGACCCTGCCTGGTGGCTAGCGGTCCCGTGTTCAGCCAAAG GTGAGGACAGAGCTGTCCAGCATTGTGACACTGGTGGGGCTAGTTTCTTCCTTGTTGGTGGCTGGGTTTC AGTCTCAGAGCCCCACCCTCACCAAGGCTCCAGGCCTCTCACAGCTCCCCCATTTATGCCTAAACATTCT CTCCTCAGAACCTCAGCTCTTAGTGAGCCACTTTTCTTGTGCAAAATGAACAATATTAAAGTTTACTACT AATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4978985 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4978985 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001974 same experiment
	EMAGE:31507 same embryo
	EMAGE:31826 same embryo
	EMAGE:31837 same embryo
	EMAGE:30603 same embryo
	EMAGE:30599 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source