EMAGE mouse gene expression spatial database

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EMAGE:31826

BC033915 ( MGI:2446296)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001952_01	euxassay_001952_02	euxassay_001952_03	euxassay_001952_04	euxassay_001952_05

euxassay_001952_06	euxassay_001952_07	euxassay_001952_08	euxassay_001952_09	euxassay_001952_10

euxassay_001952_11	euxassay_001952_12	euxassay_001952_13	euxassay_001952_14	euxassay_001952_15

euxassay_001952_16	euxassay_001952_17	euxassay_001952_18

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

moderate

regional

moderate expression: see section 01 02 03 17

vibrissa

strong

regional

strong expression: see section 18 moderate expression: see section 04 05 06

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3997
Entity Detected:	BC033915, ( MGI:2446296)
Sequence:	sense strand is shown >T3997 TGGCCTCGAGCCAGATTCGGACGAGGCAAGCGCCCACGGGGACCGTCCCCACTTGTCACCATGACACCAG CAGTGCCAGCAGTTACCCCTGTGGATGAGGAGAGCTCGGATGGGGAGCCAGATCAGGAAGCTGTGCAGAG GTAATGTGGCACCTGGCAGTGCTTGCAGAACGTCCTTGGGCAGGCTCCCCTGCAACCAGCGAAAGGTCAC CTCTGCCCAGGGAGGTGGTGCAGTGCAGCTTCAACACTTGTGTGGTCTCTTTGCTCAAGCCAGATGGCAT GTTCCTCCCCACACGGAAGTAACAGAAGACTGCCCAAAAATTTCATTTCTATAAGCGCCATTGGAAGCCC CTTAAGTGACAGCAATACCCTTAAGAACGCTTTCTCTCCTCCCTCCCTCTTCCTTGTCCCCGCGTTCATA TTGCAACTTTGGTAGTGTTCAGGCCTAACGCCTGCTCCTCCCTGCCACGAACTGTTTACTGAAAAGGAGT TGGAGATTGGAGATGACAGTGATCCTAGCTAGTCTGGCCATGGACCTCTGGCATGGACTCATCCGTCCCA
Notes:	The probe template was PCR amplified from IMAGE:596053 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:596053 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001952 same experiment
	EMAGE:31507 same embryo
	EMAGE:31837 same embryo
	EMAGE:30603 same embryo
	EMAGE:31763 same embryo
	EMAGE:30599 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source