EMAGE mouse gene expression spatial database

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EMAGE:32112

Fnbp1 ( MGI:109606)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002195_01	euxassay_002195_02	euxassay_002195_03	euxassay_002195_04	euxassay_002195_05

euxassay_002195_06	euxassay_002195_07	euxassay_002195_08	euxassay_002195_09	euxassay_002195_10

euxassay_002195_11	euxassay_002195_12	euxassay_002195_13	euxassay_002195_14	euxassay_002195_15

euxassay_002195_16	euxassay_002195_17	euxassay_002195_18	euxassay_002195_19	euxassay_002195_20

euxassay_002195_21	euxassay_002195_22	euxassay_002195_23	euxassay_002195_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 12 13

midbrain

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

thymus primordium

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13

diencephalon

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 12 13 14 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 19

hindbrain

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 16

spinal cord

weak

homogeneous

weak expression: see section 08 09 10 11

bladder

moderate

regional

moderate expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6052
Entity Detected:	Fnbp1, ( MGI:109606)
Sequence:	sense strand is shown >T6052 GCGACGAGAGGTTGCGAGCTCGGGCTAGAGGAGGCGCGGGCCGGGGGAAGCGGCGGGCTTTCCGCATTCA CAAGGTTCGGAGGGCCGCAGCGATCGCCGGCGAAGGGGGTCGGAGGCCCCTTCCCGGTCCCTGCACCATG AGCTGGGGCACTGAGCTCTGGGATCAGTTTGACAACTTGGAAAAACATACACAGTGGGGAATCGATATTC TTGAGAAATACATCAAGTTTGTCAAGGAGAGGACGGAGATTGAGCTCAGCTATGCCAAGCAACTCAGGAA TCTTTCAAAGAAATACCAACCTAAGAAGAACTCGAAGGAAGAGGAGGAGTACAAGTACACGGCTTGCAAA GCCTTTCTTTCCACCCTGAATGAGATGAATGACTACGCCGGGCAGCACGAGGTCATCTCTGAGAACATGA CGTCACAGATCACGGTGGACCTGATGCGCTACGTTCAGGAGCTGAAGCAGGAGAGGAAATCGAACTTCCA TGATGGACGGAAGGCTCAGCAGCACATAGAAACGTGTTGGAAGCAACTGGAGTCAAGTAAGAGGAGGTTT GAGCGGGACTGTAAGGAAGCCGACCGGGCACAGCAGTACTTCGAGAAAATGGACGCTGACATCAACGTGA CCAAGGCGGATGTGGAAAAGGCACGACAACAAGCTCAGATACGCCAGCAAATGGCAGAGGACAGCAAAGC AGATTACTCCTTAATCCTGCAGAGATTCAACCAGGAGCAATGGGAATACTACCATACCCACATCCCCAAC ATCTTCCAGAAAATACAAGAGATGGAGGAGAGGCGGATTGTGCGTATTGGGGAGTCCATGAAGACGTACG CAGAGGTGGACCGGCAGGTGATACCCATCATCGGGAAGTGCCTGGACGGGATAGTGAAGGCGGCCGAGTC TATCGACCAGAAAAACGACTCCCAGCTGGTCGTAGAAGCCTATAAGTCAGGATTCGAGCCTCCTGGAGAC ATTGAATTCGAAGATTACACACAGCCAATGAAACGCACAGTGTCAGACAACAGCCTTTCCAGCTCCAAAG AAGGCAAGCCTGAGCTCAGATTTGGCGGCAAGTCCAGAGGCAAGCTCTGGCCATTCATCAAGAAAAACAA GGTACTGGCCATTTGGACCCTGCGTGGGCTGTGAGCTACAGATGCAGAGTCTCAGAGTTTATCTTTTGGT CTTAATCAAACTAAGATCTGGACTTCCAGAGAGCGAGAGAGGACAGTTAGTGTGCTGCGTTCAATTCACG ATTCAAACGGTCTGTCTCTGGTGTTGGAGTCTTTGCCATGGCCTCTGTCTTATTGCAGATGCGTTGTCCC AGGACCGGGTCACCCACACTAACATCACTGACCTCACAGCCCTGTCACCCACCCCCATCCTCCCAGACTG CTGGGAGCAAGGGGACCGAGTCCCATCTAATCAAGCTTGCCAGCCTGACCTTGGTTTTGACACGTTAACC TGTCTCTGTGAATATTTTTCCCTTTTCTTTTCATAGCGTGGTATGTAGGTGCATGACAGCTAACCCGGCG GCCTAAACCGGATGGAAGTGGCCTTACTGCATTAACCTTAGATTAGACTGATTGTCTGAGTTTTCCAGGA CAGGACTGTCCCCCTGTTTTAGACCCCCTCTCCCCCATCGAACTGCGTTAGCTTAGATGTGGACGTGTTT ACCTCTGGCTTAAACTGGTTCTAGAACGACTAACGAGAGCCTAGATTGCATGTCACTGTCTGCATGAGCT TTGGGTCATGATCTTCTGCTGTCCTGTCGCGTACTCACGGACCCTCTCCTTTCCGCCCCCTCTGTGGACC GCATCCTCCATGATTTCTTTTGTACCGTGGCATCGATATTAATGGTTTGTGTTTTACTTGCTTTGGATCT TGAATATGATTTGATGCTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3490113 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490113 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002195 same experiment
	EMAGE:29869 same embryo
	EMAGE:29861 same embryo
	EMAGE:29867 same embryo
	EMAGE:32104 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source