EMAGE mouse gene expression spatial database

EMAGE:6988

Mrps24 mitochondrial ribosomal protein S24 ( MGI:1928142)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002453. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002453_01	euxassay_002453_02	euxassay_002453_03	euxassay_002453_04

euxassay_002453_05	euxassay_002453_06	euxassay_002453_07	euxassay_002453_08	euxassay_002453_09

euxassay_002453_10	euxassay_002453_11	euxassay_002453_12	euxassay_002453_13	euxassay_002453_14

euxassay_002453_15	euxassay_002453_16	euxassay_002453_17	euxassay_002453_18	euxassay_002453_19

euxassay_002453_20	euxassay_002453_21	euxassay_002453_22	euxassay_002453_23	euxassay_002453_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1984
Entity Detected:	Mrps24, mitochondrial ribosomal protein S24 ( MGI:1928142)
Sequence:	sense strand is shown >T1984 GCTACTTCCTCGTGGGCTACAGTGAGACTCTGTTGTCACACTTTTACAAGTGTCCTGTGCGACTCCACCT GCAAACTGTGCCCTCCAAGGTCGTGTACAAGTACATATAAAAAGTGCACTTTTCTCCAGTAGAGCCTACA AGGTCACAGAGGTGCAGGAGACCAAATGCCTGTTCTCTGAGGTGGAGGCGTCAGCTCCAAGCTGCTGTTG AATAAAACCCTGATCATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:718160 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:718160 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6987 same assay
	EMAGE:6989 same embryo
	EMAGE:6992 same embryo
	EMAGE:6991 same embryo
	EMAGE:6993 same embryo
	EurExpress:euxassay_002453 same experiment
	EMAGE:6990 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source