EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7011

Uck2 uridine-cytidine kinase 2 ( MGI:1931744)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002578. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002578_01	euxassay_002578_02	euxassay_002578_03	euxassay_002578_04

euxassay_002578_05	euxassay_002578_06	euxassay_002578_07	euxassay_002578_08	euxassay_002578_09

euxassay_002578_10	euxassay_002578_11	euxassay_002578_12	euxassay_002578_13	euxassay_002578_14

euxassay_002578_15	euxassay_002578_16	euxassay_002578_17	euxassay_002578_18	euxassay_002578_19

euxassay_002578_20	euxassay_002578_21	euxassay_002578_22	euxassay_002578_23	euxassay_002578_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7011_wholemount_strong.wlz

7011_wholemount_moderate.wlz

7011_wholemount_weak.wlz

7011_wholemount_possible.wlz

7011_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7011_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 04 05 06 07 08 21 22 23 weak expression: see section 24

pectoral girdle and thoracic body wall musculature

moderate

regional

moderate expression: see section 14 weak expression: see section 13

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15 16 moderate expression: see section 17

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 10 18 19 20 21

vibrissa

weak

regional

weak expression: see section 06 07 08 18 19 20 21

not examined

regional

not examined expression: see section 01 02 03 04 23 24

neural retina

moderate

regional

moderate expression: see section 01 02 03 23 24 weak expression: see section 04

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 weak expression: see section 10 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 weak expression: see section 10 16 17

orbito-sphenoid

moderate

regional

moderate expression: see section 03 04 05 06 21 22 23 24 weak expression: see section 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5192
Entity Detected:	Uck2, uridine-cytidine kinase 2 ( MGI:1931744)
Sequence:	sense strand is shown >T5192 CCACGCGTCCGCGGACGCGTGGGCGGGCACAGGAGAGCTCTCGGCACCTCCGGGAAAGCCACCTCGGCCT CCCCCGCGCCCGGGCTCCAGCCGCGGCCGGCCCAGCCTCGACCTCAGCAGCGACCAGCTGCTCCTGCGAG GAGCCGAGCGAGCCCGGTCGCGGGCGGGGAGCGTGCGTCGGTTCGCACAGGCTGCGAGAGGAGGGGCGGC GCGAGTCATGGCCGGGGACAGCGAGCAGACCCTGCAGAACCACCAGCAGCCCAACGGCGGCGAGCCCTTC CTGATCGGCGTGAGCGGGGGCACGGCCAGCGGGAAGTCTTCCGTTTGTGCTAAGATCGTCCAGCTTTTGG GGCAGAATGAGGTGGATTACCACCAGAAGCAGGTGGTGATCCTGAGCCAGGATAGCTTCTACCGAGTCCT CACCTCAGAGCAGAAGGCCAAAGCCCTCAAGGGCCAATTCAACTTTGATCACCCGGATGCCTTTGACAAC GAACTCATCTTCAAAACACTCAAAGAAATCACCGAAGGAAAAACCGTCCAGATCCCCGTATACGACTTTG TCTCCCACTCACGGAAAGAGGAGACGGTCACCATCTACCCCGCCGACGTGGTGCTCTTCGAAGGGATCCT AGCCTTCTATTCCCAGGAGGTCCGAGACCTGTTCCAGATGAAGCTTTTCGTGGACACAGATGCGGATACC CGTCTGTCTCGCCGAGTATTGAGGGACATCAGCGAAAGAGGGAGGGACCTTGAGCAGATTTTATCACAGT ACATTACGTTTGTGAAGCCTGCCTTTGAGGAATTCTGCTTGCCAACAAAGAAATACGCCGATGTGATCAT TCCTAGAGGTGCCGACAATCTCGTGGCCATCAACCTCATCGTGCAACACATCCAGGACATCCTCAACGGG GGGCTCTCCAAGCGGCAGACGAACGGCTATCTCAACGGCTACACCCCTTCCCGCAAGAGGCAGGCGTCAG AGTCCAGCAGCCGACCACATTGACTCCCGTGCCCCTGACTCCAAACCCCAGCCCCCCAGCTCCAAGACAC AGGAGGGGTCGAGAGGCTCTGGTCACCTGTACATACGCCTCCTCAGAAAATTACTGTATTTAAGAAAACA CCACGGAGATGAAATGCCTTGATTTCCCTTTCTGCCTTTTTGTACTTTGGAACAGCAAATCTTGATGGAA CTTGACCCTGAGCTTAAGTGACAAACTGTGCCAACTAGTACTGGTGATGCCTAATTATGAACCCAACGTG TAACCAGTTATAAATACACACATACATACGTCTATATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5352192 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5352192 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7010 same embryo
	EMAGE:7006 same embryo
	EMAGE:7007 same embryo
	EMAGE:7009 same embryo
	EMAGE:7008 same embryo
	EurExpress:euxassay_002578 same experiment
	MGI:4829072 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source