EMAGE mouse gene expression spatial database

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EMAGE:7036

Zbtb8b zinc finger and BTB domain containing 8b ( MGI:2387181)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002640. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002640_01	euxassay_002640_02	euxassay_002640_03	euxassay_002640_04

euxassay_002640_05	euxassay_002640_06	euxassay_002640_07	euxassay_002640_08	euxassay_002640_09

euxassay_002640_10	euxassay_002640_11	euxassay_002640_12	euxassay_002640_13	euxassay_002640_14

euxassay_002640_15	euxassay_002640_16	euxassay_002640_17	euxassay_002640_18	euxassay_002640_19

euxassay_002640_20	euxassay_002640_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7036_wholemount_strong.wlz

7036_wholemount_moderate.wlz

7036_wholemount_weak.wlz

7036_wholemount_possible.wlz

7036_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7036_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5150
Entity Detected:	Zbtb8b, zinc finger and BTB domain containing 8b ( MGI:2387181)
Sequence:	sense strand is shown >T5150 CCACGCGTCCGCCCACGCGTCCGCTGGAACCCGGTGCCAGAGCTCCAGGCCCCTCCAGAGCAGCAGCGGC TGGCGGAGAAATGGAGGCACAGTCCTACTGCGCCAAGCTCTTGGGGGAGTTGAATGAGCAGCGGAAACGA GACTTTTTCTGTGACTGCAGCATCATCGTGGAAGGGCGCATCTTCAAGGCCCACAGGAACATCCTATTCG CCAACAGTGGCTACTTCCGCGCCCTACTCCTTCACTACATCCAGGACAGCGGGCGGCACAGCACAGCTTC GCTGGACATCGTCACCTCCGACGCCTTCTCCACCATTCTAGACTTCCTCTACTCTGGGAAGTTGGATCTG TGTGGCGAGAATGTGATCGAAGTCATGTCAGCTGCCAGCTACCTGCAGATGAATGAGGTGGTCAACTTCT GTAAGACGTACATCAGGTCGTCCCTGGACATCTGCAGAAAGATGGAGAAGGAGGCCGCTGTGGCTGCCGC AATGGCGGCAGCGGCAGCTGCGGCGGCGGCTGCAGCCCATCAGATAGACAGTGAAAGTCCAAGTTCAGGA CTGGAGGGGACTTCCTGTGGCACCAAGAGCTTTGTCTCCTCCCCAGTAGATGGAGAAGGGAGTCTAGACT GTACAATATCATCTTGTGATGACTGCCATCCCTTGGAGCTGGTGGCGAAAGACAGCCAGGGCTCTGGGGT CTCCGACAATGACCTTTGTGTTGTGCCCAGACGGGTTGAACCTAAGGTGGAATTCGATGTTGCCAGGGTG GAGGTGGAGGCTGACGAACAGCTACAGCAATATGCCGCCCCACTGGCCCATATGGAGGAAGGCCTGCCCA GTAACCAGGCCTTGGATCTGACCTACAGCAGCTACCACGTGAAGCAGTTCCTGGAGGCTCTCCTGCGCAA TGGTGCTGTCCAGAGCAAAGATGACTTGGATTGTCACTCTTCTCGTGGCTTGGAGGGAAGACTGGAAGGT CCAGGAGTGGCCATGAGTTCCGTGATGGACGTTCAGAACGACTGGTATAGAGAGGACGCAGGTGACGTGC TGGTGGTTCCTATCAAGCTGCACAGGTGTCCCTTCTGCCCTTACACCGCCAAGCAGAAAGGCATCCTCAA ACGGCACATCCGCTCCCACACTGGCGAACGGCCGTACCCCTGCGAGACCTGTGGCAAGAGGTTCACCCGG CAGGAGCACCTTCGGAGTCACGCCCTGAGCGTCCACAGATCCAGCCGTCCAATTATCTGCAAAGGCTGTC GACGAACCTTTACGAGCCACCTGTCCCAAGGACTGCGGCGCTTCGGGCTGTGTGACAGTTGCACCTGTGT CACAGACCCCCAGGAGGAAGAGGACGACCTGATGCCTGTCAACCTTAGCCTGGTGGAGGCTTCATCTGAA AGCCACGAGAAGAGTGACACAGATGACTGGCCCATCTACATAGAGTCTGGTGAGGAGAATGACCCAACCG CGGAGGATTCTGACGACAAGCCACACATTCGGCCCAACTTACCAGCCCCAGAGACACTTACGTAGCAGTG ACTCGGTGGGAAGGTTTTAAGTGTTGTGGCTTGTTCTACTGTGAAAGCTACCATTTGTCGAGTTCTGTGA GACTTGGGGATCATCTCCCCACAGTGTCGTTACACTTGGATTTTTTGTTAGACGAAGGCTGGATGTTGTG ACTCAAAAAGACTGATGACGTTTTAGCATGGAGTCTTGAGAGTTTTGTTGACGTCTCCTTGGTACTGACT GAATTGAGATTGAGCCCCGAGACTTCCTAATTTTCCTGCTGAGAAGCTGACTCTGGGCCGGAGATAAATA AGAAAACCTCCCAACTTAGCACTATGCCGGTCTTTTACGTTCTTACATCATTACAGTCTTCTGAAGAGGT GGCAAGAGTATGTTGTCAGAACCTCTGTCTCTCCCCTGCCAGGCTTCTTGAAATGAACTCCACATTTAAT TTTCTGGGAATCTGCTTCGATAAAAAGCAGGCGGGGCTAACTACGTGGTCATAAAAGGTACTCCCTAAAT CTAAGACTATATGCCTAAAGGCTGGAGGAAGCTTTATGCAGACAAACACTGACACAAATTGCAAGTTTAT TCTGGGCCCCCTGCTCGCCCACCTGGATTGAGTTAAGTTAAATGAGCCCCCAGAGAAGAGCCAGTGAGCC AGGGCAGAGTACTGTCCCTGTCTACTCTGCTATTTGACAATTCCGTACACACAAATCCCCTATTCTCAGA TTATAAAGCAAGTCGTTTGGGCAAAAATCAAAAGGCTGCTTAGTCATTTCCCAGACATGCAACCTGGATA TGCACACCCTCGTGACATTCCTGGGCATGCATGTCCCGCTGACATCCTGCCACCTAGTTTATGCCCAGAC TTGAACTGCAGGGGAGACATGCATGCCTAACTAACATTCAGGTTTTAGAAGACCACCTAGAAGGAAGGAG CTGGGTAGAACAGGTTTCAAGCCGGTCTGCTTTTCACCCACTCTGCAGCCAGGTCACAACTGTAGCAAAG CAAGTCGAGTGTCTCTAAAGTATGAAAGGACAGGAAAGACCATGGATGAATGGTGCACTAGAAGGTAGCT TGCAGACTTTTCTTAAAAGCATCTGAAGTGGACTTTAAAAGAAATCCCTTGTCTTCCTTTACTTTTAACA CAGTTTCAAACAGCAGTGAAATCACCCGGTCTCTCTGCCTCTTGAACAATACCTAGTGAGAATCATTTAT AAACTGCCTTGTATGTATTGTCTTGTCATTAAAGAGTGGAAGCTTTAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5344921 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5344921 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7039 same embryo
	EMAGE:7041 same embryo
	EMAGE:7038 same embryo
	EMAGE:7040 same embryo
	EMAGE:7037 same embryo
	EurExpress:euxassay_002640 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source